Vittal Mallya Scientific Research Foundation

Bangalore, India

Vittal Mallya Scientific Research Foundation

Bangalore, India
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Manjunath B.N.,Vittal Mallya Scientific Research Foundation | Shenvi S.,Vittal Mallya Scientific Research Foundation | Raja A.,Vittal Mallya Scientific Research Foundation | Reddy G.C.,Vittal Mallya Scientific Research Foundation
Natural Product Research | Year: 2017

Though several glycosides of various triterpenes are known, but surprisingly no boswellic acid glycosides are reported so far. With a view to make water soluble boswellic acids, prepared glycosides of 11-keto boswellic acid for the first time. Naturally occurring boswellic acids which are anti-inflammatory agents are lipophylic in nature and thus, become a limiting factor in terms of their bioavailability. Among boswellic acids, 11-keto-β-boswellic acid is found to exhibit superior biological activity and hence successfully prepared its glucosyl and maltosyl derivatives viz., 11-keto-β-boswellic acid-24-O-β-D-glucopyranoside (9) and 11-keto-β-boswellic acid-24-O-α-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside (15) which are water soluble. Both these compounds are soluble in water to the extent of 10% (w/w) which is very significant. © 2017 Informa UK Limited, trading as Taylor & Francis Group

Rani A.,Institute of Himalayan Bioresource Technology | Rani A.,Vittal Mallya Scientific Research Foundation | Vats S.K.,Institute of Himalayan Bioresource Technology | Sharma M.,Institute of Himalayan Bioresource Technology | Kumar S.,Institute of Himalayan Bioresource Technology
Biologia Plantarum | Year: 2011

Catechin is associated with several functions in animal and plant systems, with little information available regarding its role in plant growth. Low concentrations of catechin (50 and 100 μM) were found to enhance length of primary and lateral roots, number of lateral roots, fresh and dry masses of shoots and roots, leaf area, water potential of leaf and root tissues, the number of vascular bundles in the inflorescence, and leaf thickness in Arabidopsis thaliana ecotype Col-0. A significant increase in net photosynthetic rate, stomatal conductance and concentration of indole-3-acetic acid was also observed in catechin treated plants. © 2011 Springer Science+Business Media B.V.

Krishnan P.,Vittal Mallya Scientific Research Foundation | Bhat R.,Vittal Mallya Scientific Research Foundation | Kush A.,Vittal Mallya Scientific Research Foundation | Ravikumar P.,Vittal Mallya Scientific Research Foundation
Journal of Applied Microbiology | Year: 2012

Aims: To isolate and characterize the endophytes from papaya fruits and to determine the fermentative potential of the strains. Methods and Results: Endophytes provide potential sources for novel natural products for the use in agriculture and nutrition. There is very limited information on isolation and characterization of bacterial endophytes from papaya. We describe isolation and characterization of eighteen endophytes of papaya fruit from four economically important papaya varieties viz 'Red lady', 'Solo', 'Coorg Honey' and 'Bangalore'. The phylogenetic analysis based on the 16S rRNA sequence revealed that isolated endophytes are genetically distinct and cluster as discrete clades in the dendrogram. The Bacillus species is a predominant bacterial endophyte across papaya varieties. The seeds and the endocarp of papaya fruits harbour Kocuria, Acinetobacter and Enterobacter species. The Staphylococcus species were detected in the fruit mesocarp of two papaya varieties used in the study. The endophytes isolated from papaya fruits were capable of producing extracellular enzymes like amylase, cellulase, pectinase and xylanase. Three isolates, Bacillus (PE-LR-1 and PE-LR-3) and Kocuria (PE-LR-2), were selected for fruit fermentation, and antioxidant potential of the fermented product was evaluated. PE-LR-3 fermented product has the free radical scavenging activity of 61·2% and a microbial cocktail of PE-LR-3 with Saccharomyces cerevisiae MTCC 2918 enhances the antioxidant potential to 75·7%. Conclusion: These findings suggest that different parts of papaya fruits harbour an array of bacterial endophytes that could be important agents in attributing the high nutritive status to the fruit and can serve as potent microbial cocktails for developing value-added fermented products of this important fruit. Significance and Impact of study: This study describes isolation of a bacterial endophyte from papaya fruit that is capable of improving the antioxidant potential of raw papaya after fermentation. © 2012 The Society for Applied Microbiology.

Shivprakash S.,Vittal Mallya Scientific Research Foundation | Reddy G.C.,Vittal Mallya Scientific Research Foundation
Synthetic Communications | Year: 2014

A synthetic method of producing (E)- and (Z)-isomers of 1-benzhydryl-4-cinnamylpiperazines in a specific ratio from corresponding benzhydrylpiperazine is described. Of the three compounds synthesized (5a-c), the ratio of E/Z-isomers remained around 15:85. The key intermediates, 1-benzhydryl-4-(2,2-dimethoxyethyl)piperazine derivatives (3a-c), were prepared by nucleophilic substitution reaction of benzhydrylpiperazines (2a-c) with chloroacetaldehyde dimethylacetal in good yield (up to 88%). Hydrolysis of 3a-c gave the corresponding aldehydes 4a-c, which when subjected to the Wittig reaction followed by column purification to afford 1a-c (E-isomers) and 6a-c (Z-isomers) in pure form. The isolated compounds were characterized by NMR and mass spectral analysis. [Supplementary materials are available for this article. Go to the publisher's online edition of Synthetic Communications® for the following free supplemental resource(s): Full experimental and spectral details.] Copyright © 2014 Taylor & Francis Group, LLC.

Srikanta Dani K.G.,Vittal Mallya Scientific Research Foundation | Srikanta Dani K.G.,University of Bristol | Hatti K.S.,Vittal Mallya Scientific Research Foundation | Ravikumar P.,Vittal Mallya Scientific Research Foundation | Kush A.,Vittal Mallya Scientific Research Foundation
Plant Biology | Year: 2011

The distinguishing structural and functional domains of plant acyl-acyl carrier protein (ACP) thioesterases and their complex interaction with the ACP-linked fatty acid substrate complex have remained elusive. E. coli based heterologous expression and characterisation of many plant thioesterases reported so far have not been extended and linked to in silico modelling studies to explain the diversity in plant thioesterase substrate specificities. In this study, a thioesterase cDNA isolated from immature seed tissues of Jatropha curcas was found to be type B and specific to stearoyl acyl ACP when expressed in E. coli K27fadD88, a lipid utilisation mutant. Homology modelling and molecular docking of a selected region of the isolated JcFatB protein predicted that it had high affinity towards both stearate (18:0) and palmitate (16:0). Structural analysis of the sequence confirmed the presence of a transit peptide that is processed in multiple steps. The enzyme is localised in the chloroplasts and has an N-terminal inner chloroplast transmembrane domain characteristic of type B plant thioesterases. Docking of ligands with JcFatB and its comparison with a modelled Jatropha thioesterase type A provided further evidence for native substrate preferences of Jatropha thioesterases. This study provides essential clues to develop future methods for large-scale bacterial production of free fatty acids and for design of strategies to modulate the seed oil composition in this important non-edible, seed oil plant. © 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.

Samaga K.K.L.,Vittal Mallya Scientific Research Foundation | Rao G.V.,Vittal Mallya Scientific Research Foundation | Chandrashekara Reddy G.,Vittal Mallya Scientific Research Foundation | Kush A.K.,Vittal Mallya Scientific Research Foundation | Diwakar L.,Vittal Mallya Scientific Research Foundation
Bioorganic Chemistry | Year: 2014

Abyssinones I and II are prenylated flavanones existing in plant Erythrina abyssinica showing diverse biological activities including anticancer activities. We synthesized racemic mixtures of these flavanones from corresponding chalcones and herein we report for the first time the molecular mechanisms of cell death, anti-proliferative effect and ability to induce apoptosis in human cervical carcinoma (HeLa) cells. Cytotoxicity was assessed by MTT assay to determine LD50 for prenylated chalcones and their corresponding flavones. Abyssinones promoted apoptosis by up regulation of p53 and Bax, along with down regulation of Bcl-2. Apoptosis induction was mediated through mitochondrial pathway releasing cytochrome c and Apaf-1 into cytosol; associated with activation of caspase-3. Further they were able to decrease the expression of cell proliferation markers PCNA and cyclin D1 indicating anti proliferative activity. These observations demonstrate that abyssinones trigger apoptosis via mitochondrial pathway by activation of caspase-3 and disrupts cell cycle. © 2014 Elsevier Ltd. All rights reserved.

Bhat R.,Vittal Mallya Scientific Research Foundation | Suryanarayana L.C.,Vittal Mallya Scientific Research Foundation | Chandrashekara K.A.,Vittal Mallya Scientific Research Foundation | Krishnan P.,Vittal Mallya Scientific Research Foundation | And 2 more authors.
Journal of Bioscience and Bioengineering | Year: 2015

Sixteen hour fermentation of the white flesh raw guava Lucknow 49 cultivar using Lactobacillus plantarum NCIM 2912 was taken up for enhancing the antioxidant potential. The fermented guava product with high antioxidant potential, total phenolic content and short and medium chain fatty acids can be used as functional food. © 2014 The Society for Biotechnology, Japan.

Dani K.G.S.,Vittal Mallya Scientific Research Foundation | Ravikumar P.,Vittal Mallya Scientific Research Foundation | Kumar R.P.,Vittal Mallya Scientific Research Foundation | Kush A.,Vittal Mallya Scientific Research Foundation
Biologia Plantarum | Year: 2011

A combination of directed amplification of minisatellite DNA (DAMD) and random amplification of polymorphic DNA (RAPD) primes were used to assess the genetic variation within and between three isolated populations of Indian sandalwood (Santalum album). Eleven primers used in this study amplified 65.99 % polymorphic bands. Analysis of molecular variance revealed a high genetic variation among these populations (φST = 0.549). There are indications of clonality within the existing Indian sandalwood populations which can be attributed to habitat fragmentation, isolation and vegetative reproduction. © 2011 Springer Science+Business Media B.V.

Rani A.,Vittal Mallya Scientific Research Foundation | Ravikumar P.,Vittal Mallya Scientific Research Foundation | Reddy M.D.,Vittal Mallya Scientific Research Foundation | Kush A.,Vittal Mallya Scientific Research Foundation
Gene | Year: 2013

Santalum album L. commonly known as East-Indian sandal or chandan is a hemiparasitic tree of family santalaceae. Santalol is a bioprospecting molecule present in sandalwood and any effort towards metabolic engineering of this important moiety would require knowledge on gene regulation. Santalol is a sesquiterpene synthesized through mevalonate or non-mevalonate pathways. First step of santalol biosynthesis involves head to tail condensation of isopentenyl pyrophosphate (IPP) with its allylic co-substrate dimethyl allyl pyrophosphate (DMAPP) to produce geranyl pyrophosphate (GPP; C10 - a monoterpene). GPP upon one additional condensation with IPP produces farnesyl pyrophosphate (FPP; C15 - an open chain sesquiterpene). Both the reactions are catalyzed by farnesyl diphosphate synthase (FDS). Santalene synthase (SS), a terpene cyclase catalyzes cyclization of open ring FPP into a mixture of cyclic sesquiterpenes such as α-santalene, epi-β-santalene, β-santalene and exo bergamotene, the main constituents of sandal oil. The objective of the present work was to generate a comprehensive knowledge on the genes involved in santalol production and study their molecular regulation. To achieve this, sequences encoding farnesyl diphosphate synthase and santalene synthase were isolated from sandalwood using suppression subtraction hybridization and 2D gel electrophoresis technology. Functional characterization of both the genes was done through enzyme assays and tissue-specific expression of both the genes was studied. To our knowledge, this is the first report on studies on molecular regulation, and tissue-specific expression of the genes involved in santalol biosynthesis. © 2013 Elsevier B.V.

Venkateswara Rao G.,Vittal Mallya Scientific Research Foundation | Karunakara A.C.,Vittal Mallya Scientific Research Foundation | Santhosh Babu R.R.,Vittal Mallya Scientific Research Foundation | Ranjit D.,Vittal Mallya Scientific Research Foundation | Chandrasekara Reddy G.,Vittal Mallya Scientific Research Foundation
Food Chemistry | Year: 2010

(-) Hydroxycitric acid lactone (HCAL) has been prepared in pure form (>98%) and converted into different salts of Group-IA and IIA metals with definite composition for the first time. The lactone is stable at room temperature but in aqueous solution it exists in equilibrium with its acid counterpart. An HPLC method has been developed to quantify (-) hydroxycitric acid (HCA) and its lactone. In-vivo studies, using male rats of the Wistar albino strain, revealed the interesting finding that HCAL exhibited better appetite suppression than did hydroxycitric acid. The continuous shift in equilibrium between HCA and HCAL due to the depletion of HCA in animal systems (slow release mechanism) could be the possible reason for this improved activity. © 2009 Elsevier Ltd. All rights reserved.

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