Virudhunagar Hindu Nadars Senthikumara Nadar College Autonomous

Virudhunagar, India

Virudhunagar Hindu Nadars Senthikumara Nadar College Autonomous

Virudhunagar, India
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Elavarasi K.,Ayya Nadar Janaki Ammal College Autonomous | Ranjini S.,Ayya Nadar Janaki Ammal College Autonomous | Rajagopal T.,Ayya Nadar Janaki Ammal College Autonomous | Rameshkumar G.,Virudhunagar Hindu Nadars Senthikumara Nadar College Autonomous | Ponmanickam P.,Ayya Nadar Janaki Ammal College Autonomous
Thai Journal of Pharmaceutical Sciences | Year: 2013

Fish skin mucus plays a major role in protection against invading pathogens. The present study was aimed to evaluate the bactericidal activity of skin mucus and skin extracts of two freshwater fishes, Clarias batrachus and Tilapia mossambicus. Fish skin mucus and skin extracts were tested against five Gram-negative organisms (Escherichia coli MTCC 443, Pseudomonas aeruginosa MTCC 3541, Aeromonas hydrophila MTCC 7639, Klebsiella pneumoniae MTCC 618, and Proteus vulgaris MTCC 426) and two Gram-positive organisms (Bacillus coagulans MTCC 492 and Staphylococcus aureus MTCC 96). The results showed that skin extracts of both fishes exhibited bactericidal activity. However, C. batrachus showed more activity against the pathogens than T. mossambicus. Protein profiles of extracts were observed by SDS-PAGE. The present investigation suggests that the mucus and skin extracts of C. batrachus and T. mossambicus are the potential sources of bactericidal proteins for pathogenic microorganisms.


Vijayalakshmi M.,Ayya Nadar Janaki Ammal College Autonomous | Rameshkumar G.,Virudhunagar Hindu Nadars Senthikumara Nadar College Autonomous | Rajagopal T.,Ayya Nadar Janaki Ammal College Autonomous | Rajagopal T.,Thiagarajar College Autonomous | And 2 more authors.
Thai Journal of Pharmaceutical Sciences | Year: 2015

The silver nanoparticles synthesized by phytofabrication method receive bigger attention due to their fascinating properties. This study deals with the green mediated synthesis of silver nanoparticles using seed extract of horse gram (Dolichos biflorus L.) and assessment of their bactericidal and antioxidant activities. The green synthesized silver nanoparticles were characterized by UV–Vis absorption spectroscopy, FTIR, EDAX, XRD and SEM. In UV- vis spectroscopic analysis, a strong peak was observed at 442 nm. The XRD pattern showed that the silver nanoparticles were crystalline nature. The average estimated particle size of the sample was 29.09 nm. Scanning Electron Microscopy (SEM) revealed that the silver nanoparticles were cubic in shape. FTIR analysis showed the possible functional groups involved in the AgNPs formation. The EDAX result exhibited a large peak of silver which confirmed its presence in the suspension. Further, the silver nanoparticles were evaluated for their antibacterial efficacy against different human pathogens by standard well diffusion method. The highest antibacterial activity was observed against Bacillus subtilis followed by Pseudomonas aeruginosa, Klebsiella sp., E. coli, Staphylococcus aureus and no activity was noticed against Proteus sp. The percentage inhibition of free radicals increased with increase in concentration of substrates. The 60 μl concentration of nanoparticles showed 99% of scavenging activity. The present study suggests that plant mediated silver nanoparticle synthesis using horse gram seed extract is quick with potential bactericidal and anti-oxidant activities. © 2015, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All right reserved.

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