Premkumar G.,Virudhunagar Hindu Nadars Senthikumara Nadar College |
Sankaranarayanan R.,Horticulture College and Research Institute |
Jeeva S.,Center for Biodiversity and Biotechnology |
Rajarathinam K.,Virudhunagar Hindu Nadars Senthikumara Nadar College
Asian Pacific Journal of Tropical Biomedicine | Year: 2011
Objective: To develop an improved protocol for micropropagation of ethnomedicinally important Scoparia dulcis (S. dulcis) L. Methods: Explants were inoculated on MS basal medium supplemented with kinetin and 6-benzylaminopurine for shoot bud induction. To enhance the shoot induction, various auxins like 3-indoleacetic acid or 3-indolebutyric acid or α-naphthylacetic acid were tested along with 2.32 M KI and 4.44 μM BAP. The regenerated shoots were rooted in half strength MS medium supplemented with various concentrations of IAA, IBA or NAA. After roots were developed, the plantlets were transplanted to pots filled with vermiculate and sand and kept in growth chamber with 70%-80% humidity under 16 h photoperiod. After acclimatization, the plantlets were transferred to the garden and survival percentage was calculated. Data were statistically analyzed and means were compared using Duncan's multiple range test (P < 0.05). Results: An in vitro method was developed to induce high frequency shoots regeneration from stem, mature leaf and young leaf explants of S. dulcis. Shoot induction on young leaf explants was most successful in MS medium supplemented with combination of two cytokinins (2.32 μM KI and 4.44 μM BAP) 2.85 μM IAA, 10% CM and 1 483.79 μM adenine sulfate. A single young leaf explant was capable of producing 59 shoots after 13 days of culture. Flower was induced in medium supplemented with combination of KI and BAP. Conclusions: Cytokinins are the key factor to induce the direct shoot regeneration and flowering of S. dulcis. © 2011 Asian Pacific Tropical Biomedical Magazine.
Jothi S.,Virudhunagar Hindu Nadars Senthikumara Nadar College |
Pandiarajan J.,Virudhunagar Hindu Nadars Senthikumara Nadar College |
Lakshmipriya V.,Virudhunagar Hindu Nadars Senthikumara Nadar College |
Prithivikumaran N.,Virudhunagar Hindu Nadars Senthikumara Nadar College |
And 2 more authors.
Special Topics and Reviews in Porous Media | Year: 2014
Researchers have shown great interest in porous silicon due to the fact that it can be fabricated with specific material characteristics. The electrical, optical, chemical, and mechanical characteristics of the material can all be modified by changing parameters of the fabrication process. The porous silicon was prepared by electrochemical etching of p-type silicon wafer where the etchant consists of a mixture of hydrofluoric acid and ethanol with various anodization current densities and 10 min etching time. The bulk modulus, Young's modulus, propagation velocities of longitudinal and transverse waves, and elastic constants are investigated. The presently investigated numerical data are found to have, in general, good agreement with the available experiment data and other such theoretical values. © 2014 by Begell House, Inc.