Virchow Biotech Pvt. Ltd
Virchow Biotech Pvt. Ltd
Chaurasia S.,Virchow Biotech Pvt. Ltd. |
Mounika K.,Virchow Biotech Pvt. Ltd. |
Mounika K.,Anurag Group of Institutions |
Bakshi V.,Virchow Biotech Pvt. Ltd. |
Prasad V.,Virchow Biotech Pvt. Ltd.
Materials Science and Engineering C | Year: 2017
The study aims at formulation and characterization of three months parenteral risperidone loaded polymeric microspheres (p-RLPMs) as a sustained delivery system and established their in vitro and in vivo assessments. The p-RLPMs formulations were prepared by solvent extraction and diffusion method. The optimized p-RLPMs (batch RPLGA-1) formulation demonstrated favorable different physicochemical properties such as mean particle size (104±5.34 μm), percent porosity (44.56±3.11%) and percent drug loading (38.42±2.67%). The physical state characterization, Fourier transformed infrared spectroscopy analysis showed no changes in the chemical structure of risperidone (RPD) in the batch RPLGA-1 formulation and differential scanning calorimetry study confirmed, pure RPD retained its crystallinity in the batch RPLGA-1 formulation. The SEM micrographs of the all p-RLPMs formulations revealed the irregular shapes and indentations. The GC/MS results showed that the residual organic solvent content in the batch RPLGA-1 formulation was below the limits. Pharmacokinetic parameters revealed that optimized RPLGA-1 formulation exhibited an initial burst followed by an excellent sustained release as compared to pure RPD as well as other formulations. Furthermore, in vivo studies of the batch, RPLGA-1 formulation showed an antipsychotic effect that was significantly prolonged over that of pure RPD solution for up to 72 h with fewer extrapyramidal side effects. Thus, results of this study prove the suitability of using poly(lactic-co-glycolic acid) copolymer to develop sustained release p-RLPMs formulations that can tailor in vivo behavior and enhance the pharmacological effectiveness of the RPD. © 2017 Elsevier B.V.
Chaurasia S.,Banaras Hindu University |
Chaurasia S.,Virchow Biotech Pvt. Ltd |
Patel R.R.,Banaras Hindu University |
Vure P.,Virchow Biotech Pvt. Ltd |
Mishra B.,Banaras Hindu University
Nanomedicine | Year: 2017
Aim: To enhance oral bioavailability and chemotherapeutic efficacy of naringenin (NG) by fabricating the NG-encapsulated Soluthin-maltodextrin-based nanocarrier (NC) system. Materials & methods: NG-encapsulated nanocarriers (NG/NCs) were developed, and in vitro physicochemically characterized. Furthermore, Wistar rats were used to evaluate the pharmacokinetic profile. Furthermore, in vitro and in vivo colorectal cancer efficacy was evaluated in BALB/c mice-bearing colon-26 cells. Results: The NG/NCs demonstrated favorable mean particle size (176 ± 2.35 nm) and percent entrapment efficiency (70.83 ± 4.55%), respectively. The oral bioavailability was found to be approximately 116-fold higher and in vitro cytotoxicity exhibited approximately 21-fold reduction as compared with pure NG. Moreover, optimized NG/NCs demonstrated significant tumor suppression compared with pure NG in vivo. Conclusion: The NG/NCs would be an efficient formulation for enhancing oral bioavailability and chemotherapeutic efficacy of NG. © 2017 Future Medicine Ltd..
Mishra B.,Banaras Hindu University |
Chaurasia S.,Virchow Biotech Pvt. Ltd.
Therapeutic Delivery | Year: 2017
Oral delivery of cancer chemotherapeutic drugs (CCDs) is subject matter in the 21st century, which changes the dosage regimens of oncotherapy with enhancement in patient's life and deducts the cost of therapy. The present report explored on the nano-oncology such as polymeric nanoparticles (PNPs) as an oral CCDs delivery vehicle, showing great potential for colon cancer treatment. Proof-of-concept in vitro and in vivo results for delivery of CCDs using various oral PNPs are included in this review from the literatures. Subsequently, the gastrointestinal barriers for oral chemotherapy have been highlighted. Furthermore, PNPs achieving better accumulation in the cancer region by desirable quality of their passive- and active-targeting phenomena have also been highlighted. © 2017 Future Science Ltd.
Kanugula A.K.R.,University of Hyderabad |
Repalle E.R.,University of Hyderabad |
Pandey J.P.,University of Hyderabad |
Sripad G.,Virchow Biotech Pvt. Ltd |
And 3 more authors.
Indian Journal of Geo-Marine Sciences | Year: 2011
Bacterial organophosphate hydrolases (OPH) have been shown to hydrolyze structurally diverse group of organophosphate (OP) compounds and nerve agents. Due to broad substrate range and unusual catalytic properties, the OPH has successfully been used to develop eco-friendly strategies for detection and decontamination of OP compounds. However, their usage has failed to gain necessary acceptance, due to short half-life of the enzyme and loss of activity during process development. In the present study, we report a simple procedure for immobilization of OPH on biocompatible gelatin pads. The covalent coupling of OPH using glutaraldehyde spacer has been found to dramatically improve the enzyme stability. There is no apparent loss of OPH activity in OPH-gelatin pads stored at room temperature for more than six months. As revealed by a number of kinetic parameters, the catalytic properties of immobilized enzyme are found to be comparable to the free enzyme. Further, the OPH-gelatin pads effectively eliminate OP insecticide methyl parathion and nerve agent sarin. © 2011, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.
Kalugonda M.K.,Virchow Biotech PVT LTD |
Venkateswarulu T.C.,Vignan University |
Kosana R.R.,Virchow Biotech PVT LTD |
Bajjji C.,Virchow Biotech PVT LTD |
And 5 more authors.
International Journal of Pharma and Bio Sciences | Year: 2013
Tumor Lysis Syndrome and Gout are the conditions in which uric acid levels in the serum will be increased up to 526 mg/dl but in a healthy human being the uric acid concentration is less than 15mg/dl. In such conditions urate oxidase can be administered parentrally which can solublise the uric acid in to allontoin which has 3 to 5 fold more solubility than uric acid and it can be excreted through urine and maintain the uric acid concentration at normal level. In this article urate oxidase gene was isolated Aspergillus flavus and it was cloned in to E. coli (BL-21). Urate oxidase gene was expressed in the cytoplasm as soluble and biologically active form. Bioprocess was optimized for 20lts fermenter scale with modified LB medium and purified up to >96% purity using fractionated ammonium sulphate precipitation, diafiltration, anion exchange chromatography, cation exchange chromatography and gel filtration chromatography. The final yield of purified recombinant urate oxidase from the 20lts fermenter was approximately 5 to 6gm of 96% pure and biologically active enzyme.
Virchow Biotech Pvt. Ltd. and Virchow Biotech Inc | Date: 2016-06-10
The present invention discloses novel devices, methods, and formulations for multiple dose delivery of an appropriate formulation of Heparin or low molecular weight heparin or a heparin-like compound. Multiple dose formulations of fondaparinux sodium, and preparation methods thereof, are also disclosed