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Oikawa A.,Joint BioEnergy Institute | Oikawa A.,Lawrence Berkeley National Laboratory | Lund C.H.,Copenhagen University | Lund C.H.,Villum Kann Rasmussen Research Center Proactive Plants | And 5 more authors.
Trends in Plant Science | Year: 2013

The plant cell wall mostly comprises complex glycans, which are synthesized by numerous enzymes located in the Golgi apparatus and plasma membrane. Protein-protein interactions have been shown to constitute an important organizing principle for glycan biosynthetic enzymes in mammals and yeast. Recent genetic and biochemical data also indicate that such interactions could be common in plant cell wall biosynthesis. In this review, we examine the new findings in protein-protein interactions among plant cell wall biosynthetic enzymes and discuss the possibilities for enzyme complexes in the Golgi apparatus. These new insights in the field may contribute to novel strategies for molecular engineering of the cell wall. © 2012.

Rosgaard L.,Copenhagen University | Rosgaard L.,Villum Kann Rasmussen Research Center Proactive Plants | de Porcellinis A.J.,Copenhagen University | de Porcellinis A.J.,Villum Kann Rasmussen Research Center Proactive Plants | And 4 more authors.
Journal of Biotechnology | Year: 2012

Development of sustainable energy is a pivotal step towards solutions for today's global challenges, including mitigating the progression of climate change and reducing dependence on fossil fuels. Biofuels derived from agricultural crops have already been commercialized. However the impacts on environmental sustainability and food supply have raised ethical questions about the current practices. Cyanobacteria have attracted interest as an alternative means for sustainable energy productions. Being aquatic photoautotrophs they can be cultivated in non-arable lands and do not compete for land for food production. Their rich genetic resources offer means to engineer metabolic pathways for synthesis of valuable bio-based products. Currently the major obstacle in industrial-scale exploitation of cyanobacteria as the economically sustainable production hosts is low yields. Much effort has been made to improve the carbon fixation and manipulating the carbon allocation in cyanobacteria and their evolutionary photosynthetic relatives, algae and plants. This review aims at providing an overview of the recent progress in the bioengineering of carbon fixation and allocation in cyanobacteria; wherever relevant, the progress made in plants and algae is also discussed as an inspiration for future application in cyanobacteria. © 2012 Elsevier B.V.

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