Vila Nova de Gaia, Portugal
Vila Nova de Gaia, Portugal

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Ribeiro H.,University of Porto | Duque L.,University of Porto | Sousa R.,University of Porto | Cruz A.,Vila Nova Of Gaia Hospitalar Center | And 3 more authors.
International Journal of Environmental Health Research | Year: 2014

This study aims to investigate the effects of O3 in protein content and immunoglobulin E (IgE)-binding profiles of Acer negundo, Platanus x acerifolia and Quercus robur pollen. Pollen was exposed to O3 in an environmental chamber, at half, equal and four times the limit value for the human health protection in Europe. Pollen total soluble protein was determined with Coomassie Protein Assay Reagent, and the antigenic and allergenic properties were investigated by SDS-PAGE and immunological techniques using patients sera.O3 exposure affected total soluble protein content and some protein species within the SDS-PAGE protein profiles. Most of the sera revealed increased IgE reactivity to proteins of A. negundo and Q. robur pollen exposed to the pollutant compared with the non-exposed one, while the opposite was observed in P. x acerifolia pollen. So, the modifications seem to be species dependent, but do not necessarily imply that increase allergenicity would occur in atopic individuals. © 2013 Taylor & Francis.


Ferreira B.,University of Porto | Ribeiro H.,University of Porto | Pereira M.S.,University of Porto | Pereira M.S.,Biosystems and Integrative science Institute | And 2 more authors.
Aerobiologia | Year: 2015

Pollen can be modified by the increasing environmental pollution which can lead to a rising trend in pollen-induced respiratory allergies. The aim of this work was to study the fertility, protein profile and allergenicity of Plantago lanceolata and Salix atrocinerea pollen after exposure to ozone. The pollen collected directly from the anthers of both species was exposed to O3 in an environmental chamber, for 6 h at different concentrations. After exposing the pollen, viability tests and in vitro germination were performed. Total protein content was determined by the Bradford method, the polypeptides were separated by SDS-PAGE and the antigenic properties were analyzed by immunological techniques using patients’ sera. The concentrations of O3 tested affected the pollen fertility and the total protein content, but the protein profiles did not show any differences. Furthermore, the immunological tests revealed an increase in IgE binding reactivity when pollen was exposed to the pollutant. © 2015 Springer Science+Business Media Dordrecht


Cuinica L.G.,University of Porto | Cruz A.,Vila Nova Of Gaia Hospitalar Center | Abreu I.,University of Porto | Da Silva J.C.G.E.,University of Porto
International Journal of Environmental Health Research | Year: 2015

Pollen of Betula pendula, Ostrya carpinifolia, and Carpinus betulus was exposed in vitro to relatively low levels of the air pollutants, namely carbon monoxide, ozone, and sulfur dioxide. The allergenicity of the exposed pollen was compared with that of non-exposed pollen samples to assess if air pollution exposition affects the allergenicity potential of pollen. The immunodetection assays indicated higher IgE recognition by all sera of allergic patients to the pollen protein extracts in all exposed samples in comparison to the non-exposed samples. These results show that the pollen exposition to low pollutants levels induces increased allergic reaction to sensitized individuals. © 2014 Taylor and Francis.


Silva M.,University of Porto | Ribeiro H.,University of Porto | Abreu I.,University of Porto | Cruz A.,Vila Nova Of Gaia Hospitalar Center | Esteves da Silva J.C.G.,University of Porto
Environmental Science and Pollution Research | Year: 2015

Atmospheric gaseous pollutants can induce qualitative and quantitative changes in airborne pollen characteristics. In this work, it was investigated the effects of carbon dioxide (CO2) on Acer negundo pollen fertility, protein content, allergenic properties, and carbohydrates. Pollen was collected directly from the anthers and in vitro exposed to three CO2 levels (500, 1000, and 3000 ppm) for 6 and 24 h in an environmental chamber. Pollen fertility was determined using viability and germination assays, total soluble protein was determined with Coomassie Protein Assay Reagent, and the antigenic and allergenic properties were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunological techniques using patients’ sera. Also, pollen fructose, sucrose, and glucose values were determined. Carbon dioxide exposure affected negatively pollen fertility, total soluble protein content, and fructose content. The patient sera revealed increased IgE reactivity to proteins of A. negundo pollen exposed to increasing levels of the pollutant. No changes were detected in the SDS-PAGE protein profiles and in sucrose and glucose levels. Our results indicate that increase in atmospheric CO2 concentrations can have a negative influence of some features of A. negundo airborne pollen that can influence the reproductive processes as well as respiratory pollen allergies in the future. © 2014, Springer-Verlag Berlin Heidelberg.

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