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Raja T.,P.A. College | Lakshmana Rao A.,Vignan Institute of Pharmaceutical Sciences
Rasayan Journal of Chemistry | Year: 2012

A new simple high performance thin layer chromatographic method for simultaneous determination of antidiabetic drugs, metformin hydrochloride and sitagliptin phosphate in bulk and tablet dosage form were investigated. Chromatographic separation of the drugs were performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of acetone:methanol:toluene:formic acid (4:3:2:1 v/v/v/v). Densitometrie evaluation of the separated zones was performed at 220 nm and the method was validated. The Rf values and drug content of metformin hydrochloride and sitagliptin phosphate were 0.36±0.02, 0.63±0.02 and 100.1%, 99.84% respectively. The calibration curves of peak area versus concentration, which were linear from 2000-5000 ng per band for metformin hydrochloride, 200-500 ng per band for sitagliptin phosphate and regression coefficient(r2) was greater than 0.99. LOD for metformin hydrochloride and sitagliptin phosphate was 45 and 27 ng per band respectively, while LOQ was 150 and 87 ng per band respectively. The method was validated for linearity, accuracy, robustness and application for assay as per ICH guidelines. The study shows that the developed method is simple and accurate and it would be suitable for the simultaneous determination of metformin hydrochloride and sitagliptin phosphate in bulk drug and pharmaceutical formulations. © 2012 RASÄYAN. All rights reserved.

Singh Sekhon B.,Vignan Institute of Pharmaceutical Sciences
Nanotechnology, Science and Applications | Year: 2014

Nanotechnology is one of the most important tools in modern agriculture, and agri-food nanotechnology is anticipated to become a driving economic force in the near future. Agri-food themes focus on sustainability and protection of agriculturally produced foods, including crops for human consumption and animal feeding. Nanotechnology provides new agrochemical agents and new delivery mechanisms to improve crop productivity, and it promises to reduce pesticide use. Nanotechnology can boost agricultural production, and its applications include: 1) nanoformulations of agrochemicals for applying pesticides and fertilizers for crop improvement; 2) the application of nanosensors/nanobiosensors in crop protection for the identification of diseases and residues of agrochemicals; 3) nanodevices for the genetic manipulation of plants; 4) plant disease diagnostics; 5) animal health, animal breeding, poultry production; and 6) postharvest management. Precision farming techniques could be used to further improve crop yields but not damage soil and water, reduce nitrogen loss due to leaching and emissions, as well as enhance nutrients long-term incorporation by soil microorganisms. Nanotechnology uses include nanoparticle-mediated gene or DNA transfer in plants for the development of insect-resistant varieties, food processing and storage, nanofeed additives, and increased product shelf life. Nanotechnology promises to accelerate the development of biomass-to-fuels production technologies. Experts feel that the potential benefits of nanotechnology for agriculture, food, fisheries, and aquaculture need to be balanced against concerns for the soil, water, and environment and the occupational health of workers. Raising awareness of nanotechnology in the agri-food sector, including feed and food ingredients, intelligent packaging and quick-detection systems, is one of the keys to influencing consumer acceptance. On the basis of only a handful of toxicological studies, concerns have arisen regarding the safety of nanomaterials, and researchers and companies will need to prove that these nanotechnologies do not have more of a negative impact on the environment. © 2014 Sekhon.

Bedre M.D.,Gulbarga University | Basavaraja S.,Jawaharlal Nehru Centre for Advanced Scientific Research | Sawle B.,Gulbarga University | Manjunath S.Y.,Vignan Institute of Pharmaceutical Sciences | Venkataraman A.,Gulbarga University
Colloids and Surfaces B: Biointerfaces | Year: 2010

In this paper, we stress upon rapid synthesis of irregular shape gold nanoparticles from a biological base. Treatment of macerated extracellular aqueous dried clove buds (Syzygium aromaticum) solution with the aqueous gold salt solution yielded irregular shaped stable gold nanoparticles in the range of 5-100. nm. The synthesis and morphology of these gold nanoparticles are understood by UV (UV-vis spectroscopy), FESEM (field emission scanning electron microscopy), TEM (transmission electron microscopy) and AFM (atomic force microscopy) techniques. The formation of these bio-adsorbed gold nanoparticles is rapid as the reaction process completes within few minutes. The XRD (X-ray diffraction studies) and EDAX (energy dispersive X-ray analysis) show that the particles are crystalline in nature. This clean-green method of synthesis is performed under ambient conditions. Probable biochemical pathway of the synthesis is studied using FTIR (Fourier transformed infrared spectroscopy). It is observed that the freely water soluble flavonoids of clove buds are responsible for bioreduction. The possible applications viz., catalysis, sensor, diagnostics, biomedical imaging and photo thermal therapy of these functionalized noble metal nanoparticles are envisaged. © 2010 Elsevier B.V.

Srinivasa Murthy M.,Vignan Institute of Pharmaceutical Sciences
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Objective: To develop and validate RP-HPLC method for estimation of Pimozide in bulk and its tablet dosage form. Method- Chromatographic separation was carried out on Grace Smart RP-18 column (250 mm X 4.6 mm inner diameter; 5μm particle size) using a mobile phase consisted of Acetonitrile: 50 mM Disodium hydrogen phosphate buffer (pH 6.2, adjusted by 1 % ortho phosphoric acid) in the ratio of 60: 40 %v/v. The flow rate was maintained at 1 ml/min and UV detection was measured at 280 nm. Propyphenazone was used as an internal standard. Results: The calibration curve of Pimozide was linear in range of 5- 100 μg/ml. The mean % assay of marketed formulation was found to be 101.02 % and % recovery was observed in the range of 99.23- 101.91 %. Relative standard deviation for precision study was found less than 2 %. The LOD and LOQ values were found to be 0.553 μg/ml and 1.678 μg/ml respectively. Conclusion: The developed method is simple rapid and easy to apply, making it suitable for routine analysis of Pimozide in bulk and tablet dosage form.

Bharti S.K.,Vignan Institute of Pharmaceutical Sciences | Kesavan K.,Vignan Institute of Pharmaceutical Sciences
Ocular Immunology and Inflammation | Year: 2016

Purpose: Moxifloxacin (MXN) is widely prescribed for the treatment of bacterial keratitis. The conventional MXN solution has several limitations, including short precorneal residence time and poor intrastromal bioavailability, requiring frequent instillation of the drug to achieve the desired therapeutic effect. To circumvent this problem, the W/O (water-in-oil) microemulsion (ME) system was utilized for sustained release and improved precorneal retention. Methods: The pseudo-ternary phase diagrams were developed and various MEs were prepared using two non-ionic surfactants, Tween 80 and Span 20, with isopropyl myristate and acetate buffer. Physicochemical parameters, in vitro drug release and in vitro antibacterial activity were studied. The in vivo antimicrobial efficacy of optimized microemulsion (ME 10) was studied in an experiment on bacterial keratitis in rabbit eyes and compared with that of the marketed eye drops. Results: The optimized microemulsion (ME 10) displays as an average globule size of <40 nm. The developed MEs showed acceptable physico-chemical behaviour, good stability for 3 months and exhibited sustained drug release. Greater efficacy in experimental bacterial keratitis in rabbit eyes was also observed in comparison with marketed drug solution. Conclusions: The developed MEs are a viable alternative to conventional eye drops, because of its ability to enhance bioavailability through its longer precorneal residence time and its ability to sustain the release of the drug. © 2016, Informa Healthcare. All rights reserved.

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