Vignan Institute of Pharmaceutical Sciences
Vignan Institute of Pharmaceutical Sciences
Kesavan K.,Banaras Hindu University |
Kesavan K.,Vignan Institute of Pharmaceutical Sciences |
Pandit J.K.,Banaras Hindu University |
Kant S.,Banaras Hindu University |
Muthu M.S.,Banaras Hindu University
Therapeutic Delivery | Year: 2013
Background: The objective of this investigation was to evaluate the potential of mucoadhesive chitosan-coated positively charged microemulsions (CH-MEs) of dexamethasone with respect to the change in nonionic cosurfactants. Methods: CH-MEs were prepared with different concentrations of surfactant and cosurfactant using the water titration method and coated with low-molecular-weight chitosan. Results: All formulations displayed an average globule size between 85 and 187 nm and a positive surface charge. The optimized CH-MEs showed greater penetration of dexamethasone in the anterior segment of the eye, resulting in twofold and fourfold higher dexamethasone concentration than uncoated ME and drug suspension, respectively. Conclusion: The developed CH-MEs shows increases in ocular penetration and bioavailability. © 2013 Future Science Ltd.
Raja T.,P.A. College |
Lakshmana Rao A.,Vignan Institute of Pharmaceutical Sciences
International Journal of PharmTech Research | Year: 2011
A method has been developed and validated for the estimation of abacavir, lamivudine and zidovudine by high performance liquid chromatography (HPLC) on a C 18 column with UV detection at 270 nm. The mobile phase composition that provides an optimal resolution of components in an acceptable elution time in water: methanol (70: 30 v/v) with 0.1% potassium dihydrogen phosphate pH 3.2 (adjusted with ortho phosphoric acid). The powdered tablet were extracted with methanol: water (50:50 v/v) mixture and after addition of stavudine, an internal standard subjected to HPLC analysis and assayed by comparison of analyte to internal standard peak areas to concentration ratios. The method was successfully applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found. The method retained its accuracy and precision when the standard addition technique was applied.
Mahesh B.D.,Gulbarga University |
Basavaraja S.,Jawaharlal Nehru Centre for Advanced Scientific Research |
Balaji S.D.,Gulbarga University |
Manjunath S.Y.,Vignan Institute of Pharmaceutical Sciences |
Venkataraman A.,Gulbarga University
Journal of Nanoparticle Research | Year: 2011
Our research interest centers on microwave- assisted rapid extracellular synthesis of biofunctionalized silver nanoparticles of 26 ± 5 nm from guava (Psidium guajava) leaf extract with control over dimension and composition. The reaction occurs very rapidly as the formation of spherical nanoparticles almost completed within 90 s. The probable pathway of the biosynthesis is suggested. Appearance, crystalline nature, size and shape of nanoparticles are understood by UV-vis (UV-vis spectroscopy), FTIR (fourier transform infrared spectroscopy), XRD (X-ray diffraction), FESEM (field emission scanning electron microscopy) and TEM (transmission electron microscopy) techniques. Microwave-assisted route is selected for the synthesis of silver nanoparticles to carry out the reaction fast, suppress the enzymatic action and to keep the process environmentally clean and green. © Springer Science+Business Media B.V. 2010.
Mishra A.,Vignan Institute of Pharmaceutical Sciences |
Gupta P.,Vignan Institute of Pharmaceutical Sciences
International Journal of Drug Development and Research | Year: 2012
In recent years several advancement has been made in research and development of Oral Drug Delivery System. Concept of Novel Drug Delivery System arose to overcome the certain aspect related to physicochemical properties of drug molecule and the related formulations. Purpose of this review is to compile the recent literature with special focus on various gastro retentive approaches that have recently become leading methodologies in the field of site-specific orally administered controlled release drug delivery. Technological attempts have been made in the research and development of ratecontrolled oral drug delivery systems to overcome physiological adversities, such as short gastric residence times (GRT) and unpredictable gastric emptying times (GET). Therefore, gastro retentive drug delivery systems (GRDDS) have been developed, which prolong the gastric emptying time. Several techniques such as floating drug delivery system, low density systems, raft systems, mucoadhesive systems, high density systems, super porous hydro gels and magnetic systems, have been employed. This review on GRDDS attempts to compile the available information with all the possible mechanism used to achieve gastric retention. © 2012 IJDDR, Amul Mishra & Pinky Gupta et al.
Debnath M.,Vignan Institute of Pharmaceutical Sciences
Indian Drugs | Year: 2016
A new RP-HPLC method for the quantitative determination of aceclofenac in albino rat plasma was developed and validated as per US-FDA guidelines. The drug was spiked in the plasma and extracted with mobile phase by precipitation method. The extracted analyte was injected into XTerra C18 (4.6 × 250 mm; 5 μrn) or equivalent, maintained at 25°C temperature and effluent was monitored at 274 nm. The mobile phase consisted of potassium dihydrogen phosphate [pH 4.5]: methanol: acetonitrile [HPLC grade] (55:25:20 (V/V). The flow rate was maintained at 1.0 mL/min. The developed method showed high specificity for aceclofenac. The calibration curve for aceclofenac was linear from 25.0 to 125.0 ng/0.5ml-plasma(r2= 0.999). The inter-day and intra-day precision was found to be within limits. The average % recovery for aceclofenac was found to be 99.74-99.77 % and the reproducibility was found to be satisfactory.The proposed method was adequate, sensitivity, reproducibility, and specificity for the determination ofaceclofenac in albino ratplasma.
Agarwal S.,Vignan Institute of Pharmaceutical Sciences |
Kamala Kumari P.V.,Vignan Institute of Pharmaceutical Sciences |
Srinivasa Rao Y.,Vignan Institute of Pharmaceutical Sciences
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013
Objective: The objective of the present study was to formulate, evaluate and compare the dissolution profiles of Eslicarbazepine acetate (ESL) immediate release tablets using natural binders like tapioca starch, maize starch and galbanum, against the synthetic binder like Polyvinyl Pyrrolidone (PVP). Methods: These natural binders were used as novel excipients which formed weak attractive bonds with the drug by complexation during wet granulation and increased the solubility of ESL. Each of these natural and synthetic binders was used separately in different concentrations to prepare immediate release tablets of ESL. Dissolution profiles of these tablets were compared. Results: The in-vitro drug release of F4 (with galbanum 1%) was highest 98.78%, whereas the least drug release of 61.66% was shown by F12 (with PVP 3%). Conclusion: It was seen that the tablets with natural binders showed better release characteristics in comparison to the tablets with synthetic binder PVP. Formulation with galbanum 1% showed best release profile. Galbanum also has antiepileptic property which can act synergistically with Eslicarbazepine acetate to treat seizures. It was also seen that among the various concentrations of binders used formulations with 1% binder showed better release characteristics when compared to 2% and 3%.
Damodar R.,Vignan Institute of Pharmaceutical Sciences |
Movva B.,Vignan Institute of Pharmaceutical Sciences
Journal of Bioequivalence and Bioavailability | Year: 2014
The purpose of present investigation was to develop the dosage form containing metformin for both immediate and sustained release. The SR release tablets of metformin were not useful to control the fasting glucose levels whereas conventional metformin tablets cannot acts for prolonged time, But the tablets prepared by present method useful for control both fasting glucose levels and maintenance dose. Even though many combination therapies available in market as metformin for sustain release and other sulfonylureas for immediate release, The primary concern for considering metformin hydrochloride as monotherapy was its efficient activity, less cost and negligible cardiac risk factors. The immediate release dose was developed by direct compression method and sustained release beads were prepared by inotropic gelation method using sodium alginate and sodium CMC, CaCl2. The various batches of directly compressed tablets with different percentages of sustained release beads were prepared and evaluated for various physical properties and dissolution profile. Hardness (kg/cm2) of tablets was decreased and percentage loss in friability is increased as concentration of beads in tablet increased. All the parameters are within range for tablets containing micro beads up to 35% thereafter loss in friability and Hardness (kg/cm2) are not within range. © 2014 Damodar R, et al.
Debnath M.,Vignan Institute of Pharmaceutical Sciences
Indian Drugs | Year: 2017
A new RP-HPLC method for the quantitative determination of tinidazole in albino rat plasma was developed and validated as per US-FDA guidelines. The drug was spiked in the albino rat plasma and extracted with mobile phase by precipitation method. The extracted analyte was injected into Hypersil ODS C18 (4.6 x 150 mm; 5 μm) or equivalent, maintained at 25°C temperature and effluent monitored at 310 nm. The mobile phase consisted of potassium dihydrogen phosphate [pH 3.0]: acetonitrile [HPLC Grade] (40:60 v/v). The flow rate was maintained at 1.0 mL/min. The developed method shows high specificity for tinidazole. The calibration curve for tinidazole was linear from 1.0 to 40.0 ng/0.5mL plasma (r2= 1). The inter-day and intra-day precision was found to be within limits. The average % recovery for the drug tinidazole was found to be 99.53-100.21 % and the reproducibility was found to be satisfactory. The proposed method was adequate, sensitivity, reproducibility, and specificity for the determination of tinidazole in albino rat plasma.
Bharti S.K.,Vignan Institute of Pharmaceutical Sciences |
Kesavan K.,Vignan Institute of Pharmaceutical Sciences
Ocular Immunology and Inflammation | Year: 2016
Purpose: Moxifloxacin (MXN) is widely prescribed for the treatment of bacterial keratitis. The conventional MXN solution has several limitations, including short precorneal residence time and poor intrastromal bioavailability, requiring frequent instillation of the drug to achieve the desired therapeutic effect. To circumvent this problem, the W/O (water-in-oil) microemulsion (ME) system was utilized for sustained release and improved precorneal retention. Methods: The pseudo-ternary phase diagrams were developed and various MEs were prepared using two non-ionic surfactants, Tween 80 and Span 20, with isopropyl myristate and acetate buffer. Physicochemical parameters, in vitro drug release and in vitro antibacterial activity were studied. The in vivo antimicrobial efficacy of optimized microemulsion (ME 10) was studied in an experiment on bacterial keratitis in rabbit eyes and compared with that of the marketed eye drops. Results: The optimized microemulsion (ME 10) displays as an average globule size of <40 nm. The developed MEs showed acceptable physico-chemical behaviour, good stability for 3 months and exhibited sustained drug release. Greater efficacy in experimental bacterial keratitis in rabbit eyes was also observed in comparison with marketed drug solution. Conclusions: The developed MEs are a viable alternative to conventional eye drops, because of its ability to enhance bioavailability through its longer precorneal residence time and its ability to sustain the release of the drug. © 2016, Informa Healthcare. All rights reserved.
Singh Sekhon B.,Vignan Institute of Pharmaceutical Sciences
Nanotechnology, Science and Applications | Year: 2014
Nanotechnology is one of the most important tools in modern agriculture, and agri-food nanotechnology is anticipated to become a driving economic force in the near future. Agri-food themes focus on sustainability and protection of agriculturally produced foods, including crops for human consumption and animal feeding. Nanotechnology provides new agrochemical agents and new delivery mechanisms to improve crop productivity, and it promises to reduce pesticide use. Nanotechnology can boost agricultural production, and its applications include: 1) nanoformulations of agrochemicals for applying pesticides and fertilizers for crop improvement; 2) the application of nanosensors/nanobiosensors in crop protection for the identification of diseases and residues of agrochemicals; 3) nanodevices for the genetic manipulation of plants; 4) plant disease diagnostics; 5) animal health, animal breeding, poultry production; and 6) postharvest management. Precision farming techniques could be used to further improve crop yields but not damage soil and water, reduce nitrogen loss due to leaching and emissions, as well as enhance nutrients long-term incorporation by soil microorganisms. Nanotechnology uses include nanoparticle-mediated gene or DNA transfer in plants for the development of insect-resistant varieties, food processing and storage, nanofeed additives, and increased product shelf life. Nanotechnology promises to accelerate the development of biomass-to-fuels production technologies. Experts feel that the potential benefits of nanotechnology for agriculture, food, fisheries, and aquaculture need to be balanced against concerns for the soil, water, and environment and the occupational health of workers. Raising awareness of nanotechnology in the agri-food sector, including feed and food ingredients, intelligent packaging and quick-detection systems, is one of the keys to influencing consumer acceptance. On the basis of only a handful of toxicological studies, concerns have arisen regarding the safety of nanomaterials, and researchers and companies will need to prove that these nanotechnologies do not have more of a negative impact on the environment. © 2014 Sekhon.