Svidova S.,University of Vienna |
Sponder G.,University of Vienna |
Schweyen R.J.,University of Vienna |
Djinovic-Carugo K.,University of Vienna |
And 2 more authors.
Biochimica et Biophysica Acta - Biomembranes | Year: 2011
The Leu294 residue in the cytoplasmic neck of Thermotoga maritima CorA is considered to be the main gate for Mg2+ transport. We created three site-directed mutants at this position: in the Leu294Asp and Leu294Gly mutants we observed a defect in closing of the pore, while in the Leu294Arg mutant not only gating, but also the regulation of Mg2+ uptake was affected. Our results confirmed the importance of the Leu294 for gating of Mg2+ transport and in addition revealed the influence of the charge and structural features of the amino acid residues on the gating mechanism. © 2010 Elsevier B.V. All rights reserved.