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Roubalova K.,Vidia Ltd | Strunecky O.,National Institute of Public Health | Vitek A.,Institute of Haematology and Blood Transfusion | Zufanova S.,National Institute of Public Health | Prochazka B.,National Institute of Public Health
Transplant Infectious Disease | Year: 2011

Genetic variation of cytomegalovirus (CMV) strains can correlate with their pathogenicity for immunocompromised patients. Glycoprotein O (gO), together with glycoprotein L and glycoprotein H, mediate the fusion of the viral envelope with the cell membrane and promotes virus penetration, envelopment, and release. The variability of gO might play a role in CMV cell tropism. The goal was a retrospective analysis of gO variability in a cohort of hematopoietic stem cell transplant (HSCT) recipients to determine the distribution of gO genotypes and to investigate their impact on clinical outcome and manifestation of CMV infection. Methods. In archived blood samples from 51 adult allogeneic HSCT recipients with active CMV infection, gO was analyzed by sequencing the N-terminal domain of the UL74 gene using the dye deoxy termination method. Results. The gO1 and gO2 clades were most common (39% and 20%, respectively, and gO3 was associated with higher risk of symptomatic infection (P=0.026 in multivariant analysis). Despite being associated with higher antigenemia levels (P=0.02), gO4 had the best survival and lower rate of CMV recurrence. No significant differences were found in clinical manifestation and outcome of CMV disease between patients with various gO clades. Because CMV strains sharing an identical gO sequence differed in glycoprotein B genotypes, sequencing the N-terminal part of the gO gene does not seem to be optimal for the identification of strains. Conclusions. gO genotyping may contribute to the biological characterization of CMV strains in HSCT recipients. © 2011 John Wiley & Sons A/S.


Pauk N.,Charles University | Klimesova S.,Charles University | Kara J.,Charles University | Topinka J.,Academy of Sciences of the Czech Republic | Labaj J.,Vidia Ltd
Neoplasma | Year: 2013

Certain substances from the polycyclic aromatic hydrocarbons (PAHs) group are major inducers of respiratory tract carcinogenesis. The presented are the results of a serological epidemiological study aimed at monitoring the levels of anti-PAH antibodies and antibodies to PAH-DNA adducts in serum. The patients studied belonged both to the group of those with known lung disease (COPD and lung cancer), as well as to the healthy population of people who due to the work conditions or those at the place of residence can expect increased exposure to PAHs. In addition to the results proper that confirm increase of the genotoxic exposure risk to PAH in smoke-polluted places of residence and other PAH polluted environments. There has also been proved the relevance of still commonly used markers (DNA adducts), as well as the suitability of new markers, more favourable from the economic and practical viewpoints (anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA [anti-BPDE-DNA], anti-Benzo(a)pyrene antibodies of the IgA class).


Cernohorska H.,Vidia Ltd. | Cernohorska H.,University of Pardubice | Klimesova S.,University Hospital Bulovka | Lepsa L.,Vidia Ltd. | And 5 more authors.
Mutation Research - Genetic Toxicology and Environmental Mutagenesis | Year: 2012

Polycyclic aromatic hydrocarbons (PAH) are recognized as common environmental pollutants released into the environment from many natural as well as man-made sources, and some have been classified as potent carcinogens. The main representative of the carcinogenic PAH is benzo(a)pyrene (B(a)P) which is known to induce genotoxic effects in vitro and in vivo, detected as PAH-DNA adducts. Long-term PAH exposure may be accompanied by an immunological response with the formation of antibodies against PAH as well as against PAH-DNA adducts. This paper describes the use of four PAH-keyhole-limpet haemocyanin (KLH) conjugates for the induction of specific and cross-reactive anti-PAH antibodies and focuses on the potential protective effects of anti-PAH antibodies produced after immunization of mice. In the in vitro experiments with HepG-2 cells, the genotoxicity of the PAH-KLH conjugates and the neutralizing effect of induced anti-PAH antibodies were evaluated. The titer of specific anti-PAH antibodies in sera and the amounts of DNA adducts in liver homogenates from immunized mice were investigated in vivo. The results show that anti-PAH antibodies of class IgG were induced during immunization. All the PAH-KLH conjugates tested were non-genotoxic and did not induce detectable DNA adducts in HepG2 cells or in the liver of immunized mice. The results show that only B(a)P-specific and B(a)P cross-reactive antibodies are able to neutralize B(a)P or its activated metabolites, which was revealed by a sudden decrease in the titer of anti-B(a)P antibodies in mouse sera after exposure to B(a)P. Furthermore, the anti-B(a)P antibodies produced by immunization were effective in reducing the amount of DNA adducts in mouse livers after intraperitoneal (i.p.) exposure to B(a)P. The results suggest that immunization with PAH-KLH conjugates can protect organisms against the adverse effects of carcinogenic PAH. © 2011 Elsevier B.V.


Kosikova B.,Chemicky ustav SAV | Labaj J.,VIDIA Ltd
Acta Facultatis Xylologiae | Year: 2011

Lignin belongs to the main components of plant biomass. It is obtained as a by-product in the paper production and biomass-conversion technologies. Potential use of lignin preparations as antioxidants results from the presence of unique hindered phenolic hydroxyl groups which can act as radical scavenger. The cytotoxicity of lignin preparations isolated from spent liquors was examined by Plating efficiency as well as by Trypan blue exclusion technique on mammalian cells (hamster V79, human CaCo-2 and LEP cells). The cytotoxicity of lignins vary with wood species, methods of isolation and fractionation. Their genotoxicity was determined by comet assay. The in vitro activity lignin preparations was tested in mice lymphocytes and testicular cells against DNA damage induced by hydrogen peroxide. In the cells preincubated with lignin a significant decrease of DNA lesions induced by H2O2 can be observed in comparison with control sample. The ability of the lignin antioxidants to protect organisms against the development of cancer was examined on lignin fed rats in ex vivo experiments. The obtained results show that the used lignin sample decreased the level of strand breaks significantly in rat lymphocytes and testicular cells, i.e. their resistance to oxidative stress induced by H2O2 was increased. The revealed antigenotoxic effect of the isolated lignins in vitro and ex vivo shows new way of valorization and utilization of lignin waste products from pulp and paper industry for antitumor use. The results obtained by determination of cytotoxicity of lignins and by the experiments with laboratory animals indicate that non-toxic lignin preparations have potential to protect living organisms against damaging effects of different genotoxicants instead of synthetic antioxidants.


Trademark
VIDIA Inc | Date: 2015-02-09

Publications, namely, periodicals, magazines, newspapers, books, newsletters, journals, photographs, brochures, flyers, guides in the fields of general interests, news, leisure, lifestyles, fashion, music, politics, art, television, film, commentary, culture, business, sports, entertainment, events, history, travel, humor, satire. Publishing of electronic publications, articles and blogs; Entertainment services in the nature of live visual and audio performances by actors and performers; Entertainment, namely, production of shows, news and commentaries, photographic and prose presentations, radio and television programs, video, films, television shows and movies, music videos and recordings distributed via various platforms across multiple forms of media; Providing a website featuring blogs and non-downloadable publications in the nature of articles and stories in the field(s) of general interests, news, leisure, lifestyles; Entertainment services in the nature of musical group, television ethnic series, ethnic festival.


PubMed | Vidia Ltd
Type: Journal Article | Journal: Transplant infectious disease : an official journal of the Transplantation Society | Year: 2011

Genetic variation of cytomegalovirus (CMV) strains can correlate with their pathogenicity for immunocompromised patients. Glycoprotein O (gO), together with glycoprotein L and glycoprotein H, mediate the fusion of the viral envelope with the cell membrane and promotes virus penetration, envelopment, and release. The variability of gO might play a role in CMV cell tropism. The goal was a retrospective analysis of gO variability in a cohort of hematopoietic stem cell transplant (HSCT) recipients to determine the distribution of gO genotypes and to investigate their impact on clinical outcome and manifestation of CMV infection.In archived blood samples from 51 adult allogeneic HSCT recipients with active CMV infection, gO was analyzed by sequencing the N-terminal domain of the UL74 gene using the dye deoxy termination method.The gO1 and gO2 clades were most common (39% and 20%, respectively, and gO3 was associated with higher risk of symptomatic infection (P = 0.026 in multivariant analysis). Despite being associated with higher antigenemia levels (P = 0.02), gO4 had the best survival and lower rate of CMV recurrence. No significant differences were found in clinical manifestation and outcome of CMV disease between patients with various gO clades. Because CMV strains sharing an identical gO sequence differed in glycoprotein B genotypes, sequencing the N-terminal part of the gO gene does not seem to be optimal for the identification of strains.gO genotyping may contribute to the biological characterization of CMV strains in HSCT recipients.

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