Wu V.W.-K.,National Kaohsiung University of Applied Sciences |
Wu V.W.-K.,Victor Basic Research Laboratory |
Kure F.,Victor Basic Research Laboratory
Chinese Journal of Chemistry | Year: 2010
Solutions of suspended particles of nanodiamond and nanosilica in deionized water 25 mg/10 mL were prepared for the purpose of achieving saturation adsorption. The respective saturation thresholds and adsorption reaction constants of lysozyme of extremely diluted concentrations 0-1000 nmol/L in 7 mmol/L (potassium phosphate buffer solution) PPBS on the surfaces of nanodiamond and nanosilica of 100 nm diameter have been measured. The adsorbed quantities of lysozyme on a unit surface area of both nanoparticles can be derived. The possible influences by the adsorption surface profile and adsorption surface area, adsorption ability of the nanoparticles, strength and activity of lysozyme have been discussed. Lysozyme molecules lay on the surfaces of nanoparticles and can keep their helicities as well as activities of 90% if they are optimally loaded on the surface of nanodiamond (ND) at 150 or around threshold 190 nmol/L, and on the surface of nanosilica at 700 nmol/L of lysozyme concentrations. The rough surface of ND causes that the adsorbed lysozyme molecule can better keep its helicity, even at extremely diluted concentration, and shows higher adsorption capability. © 2010 SIOC, CAS, Shanghai & WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.