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Socoteanu R.,Ilie Murgulescu Institute of Physical Chemistry | Manda G.,Victor Babes National Institute for Pathology and Biomedical science | Boscencu R.,Carol Davila University of Medicine and Pharmacy | Vasiliu G.,Carol Davila University of Medicine and Pharmacy | And 2 more authors.
Molecules | Year: 2015

In this paper, two tetrapyrrolic complexes, Zn(II)-5-(3-hydroxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl)porphyrin and Cu(II)-5-(3-hydroxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl)porphyrin were synthesized, and characterized from a spectral and biological point of view. The study provided data concerning the behavior of identical external substituents vs. two different core insertions. Some of the properties of the proposed tetrapyrrolic structures were highlighted, having photodynamic therapy of cancer as a targeted biomedical application. Elemental analysis, NMR, FTIR and UV-Vis data in various solvents were provided. A preliminary in vitro study on normal and cancer cultured cells was carried out for biocompatibility assessment in dark conditions. The preliminary in vitro study performed on human peripheral mononuclear cells exposed to tetrapyrrolic compounds (2 μM) showed that the proposed compounds had a convenient cytotoxic profile on human normal peripheral blood mononuclear cells under dark conditions. Meanwhile, the investigated compounds reduced the number of metabolically active breast tumor MCF-7 cells, with the exception of Zn(II) complex-containing a symmetrical ligand. Accordingly, preliminary in vitro data suggest that the proposed tetrapyrrolic compounds are good candidates for PDT, as they limit tumor expansion even under dark conditions, whilst sparing normal cells. © 2015 by the authors; licensee MDPI, Basel, Switzerland.

Cojocaru I.,Stefan Cel Mare University of Suceava | Cojocaru M.,Center for Rheumatic Diseases | Silosi I.,University of Medicine and Pharmacy of Craiova | Vrabie C.,Victor Babes National Institute for Pathology and Biomedical science
Journal of Medical Biochemistry | Year: 2011

Patients with multi-system rheumatic conditions may have a disease affecting the central nervous system (CNS). Central nervous system manifestations vary according to the location of the lesion and range from focal findings (e.g., stroke-like presentations), although serious neurological complications in rheumatic disease appear to be rare. The most prominent features of neurological involvement in rheumatic diseases include cerebral ischaemia and psychiatric symptoms. Little information is available on the prevalence of neurological disease in patients with a rheumatological diagnosis. Involvement of the CNS may be a striking early or presenting feature with a wide variety of manifestations. There is more clarity about the CNS syndromes attributable to systemic lupus erythematosus and new insights into the central mechanisms involved in the manifestations of Sjögren's syndrome and rheumatoid arthritis. Severe CNS involvement is associated with poor prognosis, and high mortality rate. We review the spectrum of neurological diseases in patients with a rheumatological diagnosis.

Socoteanu R.,Ilie Murgulescu Institute of Physical Chemistry | Anastasescu M.,Ilie Murgulescu Institute of Physical Chemistry | Oliveira A.,Polytechnic Institute of Coimbra | Oliveira A.,University of Lisbon | And 3 more authors.
International Journal of Photoenergy | Year: 2015

Fractal analysis of free bases porphyrins was computed on atomic force microscopy (AFM) micrographs using two different methods: the correlation function method and the variable length scale method. The correlation function method provides fractal dimension only for short scale range; results indicate that only few images have fractal properties for short ranges; for the rest of them, no fractal dimension was found using the correlation function method. The variable length scale method occur information for long range scaling. All samples have fractal properties at higher scaling range. For three samples the correlation function method leads to the same fractal dimension as the variable length scale method and scaling ranges for both methods overlap. Results show the necessity to use both methods to describe the fractal properties of AB3 meso-porphyrins that may be used to predict their relative cell localization. In order to emphasize the influence of fractal and textural properties the results regarding their self-similarity and texture/morphology were further compared with their behavior in biological assessment, that is, functionality of some Jurkat cell lines. © 2015 Radu Socoteanu et al.

Vieira Ferreira L.F.,University of Lisbon | Ferreira D.P.,University of Lisbon | Oliveira A.S.,University of Lisbon | Oliveira A.S.,Polytechnic Institute of Portalegre | And 5 more authors.
Dyes and Pigments | Year: 2012

A series of some A 3B type mesoporphyrinic compounds were synthesized with superior yields using the microwave irradiation. The prepared compounds were characterized by elemental analysis, FT-IR, UV-Vis, NMR and EPR spectroscopy, which fully confirmed their structures. The spectral molecular absorption properties of the porphyrinic compounds were studied in different solvents and the influence of the solvent polarity on the absorbance maxima was described. Fluorescence emission and singlet oxygen formation quantum yields were evaluated for A 3B type mesoporphyrinic compounds and compared with the corresponding symmetrical compounds, revealing high yields for the metal free compounds, followed by the zinc derivatives. The copper mesoporphyrinic compounds are not emissive and only evidence residual capacity for singlet oxygen formation. In order to establish their future potential in biomedical application preliminary toxicological studies, consisting of viability and proliferation of living cells in the presence of the unsymmetrical mesoporphyrinic compounds, were performed in the dark, on a standard cell line of human Caucasian histiocytic lymphoma (U937). The obtained results indicate a very low or no cytotoxicity at all for all compounds under study. Therefore further testing with light activation protocols is recommended in future work. © 2012 Elsevier Ltd. All rights reserved.

Constantin C.,Victor Babes National Institute for Pathology and Biomedical science | Neagu M.,Victor Babes National Institute for Pathology and Biomedical science | Ion R.-M.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Ion R.-M.,University Valahia of Targoviste | And 2 more authors.
Nanomedicine | Year: 2010

Photodynamic therapy represents an alternative treatment with great potential in some types of cancer and premalignant conditions. In the quest to improve this therapy, potential new nontetrapyrrole photosensitizers are currently under research. Hence, in the last few years fullerenes attracted an increased interest because they prove characteristics for nanotechnologys biomedical applications. Fullerenes derivatization for biology application in general and in particular for photodynamic therapy, led to the idea of their association with porphyrins. Porphyrins, well-known players in this domain, could form in association with fullerenes, new compounds with unique properties, namely new photosensitizers with enhanced efficiency in terms of singlet oxygen generation and tumor cell penetration. This article is an attempt to underscore the enormous effort currently dedicated to an emerging field represented by these new nanostructures for biomedicine and in particular for photodynamic therapy. © 2010 Future Medicine Ltd.

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