Time filter

Source Type

Ion R.M.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Ion R.M.,University Valahia of Targoviste | Fierascu R.C.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Fierascu R.C.,University Valahia of Targoviste | And 3 more authors.
Science of Advanced Materials | Year: 2010

The unique shape of the fullerene C60 make them good candidates for preparation of supramolecular assemblies with different molecules, as porphyrins. The purpose of our study is the in vitro analysis of the triad tetraphenyl-porhyrin (TPP)-poly(vinylpyrrolidone) (PVP)-fullerene (C60)-(TPPPVP -C60). The cell viabilities before and after irradiations with TPP-PVP-C60 triad has been tested on K562 (leukemia cell line). The uptake of sensitizers into K562 (leukemia cell line) was studied using 1 microM of sensitizer and different times of incubation. In biological medium, no dark cytotoxicity was observed using sensitizer concentrations 0.05-50 microM and 1-18 h of incubation. The cell survival after irradiation of the cells with visible light was dependent upon light-exposure level. A higher photocytotoxic effect was observed for TPP-PVP-C60 triad, which inactivates 80% of cells after 15 min of irradiation. These results show that molecular triad, are a promising model for phototherapeutic agents, with potential applications in cell inactivation by PDT. © 2010 American Scientific Publishers.


Fierascu R.C.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Fierascu R.C.,University Valahia of Targoviste | Dumitriu I.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Dumitriu I.,University Valahia of Targoviste | And 5 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2010

The nanoengineering of various materials for biomedical application has became in the last decade one of the most important research areas, due to the continuous struggle to find new and more efficient instruments for the therapy of cancer and other diseases. Attempts to obtain functionalized derivatives of fullerene seek the synthesis of water-soluble materials, in order to investigate their effects in physiological conditions. International studies on the biological properties of fullerenes and their derivatives, are now targeted towards anti-tumor effects, pharmacology and their involvement in oxidative stress. Their toxicity, demonstrated both in vitro and in vivo is important for characterization and selection of applications. Phototoxicity of some molecules of fullerenes has been identified as future therapeutic tool. The present paper describes the synthesis, characterization and some biomedical applications of some nanomaterials based on fullerenes. © 2010 SPIE.


Constantin C.,Victor Babes National Institute for Pathology and Biomedical science | Neagu M.,Victor Babes National Institute for Pathology and Biomedical science | Ion R.-M.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Ion R.-M.,University Valahia of Targoviste | And 2 more authors.
Nanomedicine | Year: 2010

Photodynamic therapy represents an alternative treatment with great potential in some types of cancer and premalignant conditions. In the quest to improve this therapy, potential new nontetrapyrrole photosensitizers are currently under research. Hence, in the last few years fullerenes attracted an increased interest because they prove characteristics for nanotechnologys biomedical applications. Fullerenes derivatization for biology application in general and in particular for photodynamic therapy, led to the idea of their association with porphyrins. Porphyrins, well-known players in this domain, could form in association with fullerenes, new compounds with unique properties, namely new photosensitizers with enhanced efficiency in terms of singlet oxygen generation and tumor cell penetration. This article is an attempt to underscore the enormous effort currently dedicated to an emerging field represented by these new nanostructures for biomedicine and in particular for photodynamic therapy. © 2010 Future Medicine Ltd.


Cojocaru I.,Stefan Cel Mare University of Suceava | Cojocaru M.,Center for Rheumatic Diseases | Silosi I.,University of Medicine and Pharmacy of Craiova | Vrabie C.,Victor Babes National Institute for Pathology and Biomedical science
Journal of Medical Biochemistry | Year: 2011

Patients with multi-system rheumatic conditions may have a disease affecting the central nervous system (CNS). Central nervous system manifestations vary according to the location of the lesion and range from focal findings (e.g., stroke-like presentations), although serious neurological complications in rheumatic disease appear to be rare. The most prominent features of neurological involvement in rheumatic diseases include cerebral ischaemia and psychiatric symptoms. Little information is available on the prevalence of neurological disease in patients with a rheumatological diagnosis. Involvement of the CNS may be a striking early or presenting feature with a wide variety of manifestations. There is more clarity about the CNS syndromes attributable to systemic lupus erythematosus and new insights into the central mechanisms involved in the manifestations of Sjögren's syndrome and rheumatoid arthritis. Severe CNS involvement is associated with poor prognosis, and high mortality rate. We review the spectrum of neurological diseases in patients with a rheumatological diagnosis.


Socoteanu R.,Ilie Murgulescu Institute of Physical Chemistry | Manda G.,Victor Babes National Institute for Pathology and Biomedical science | Boscencu R.,Carol Davila University of Medicine and Pharmacy | Vasiliu G.,Carol Davila University of Medicine and Pharmacy | And 2 more authors.
Molecules | Year: 2015

In this paper, two tetrapyrrolic complexes, Zn(II)-5-(3-hydroxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl)porphyrin and Cu(II)-5-(3-hydroxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl)porphyrin were synthesized, and characterized from a spectral and biological point of view. The study provided data concerning the behavior of identical external substituents vs. two different core insertions. Some of the properties of the proposed tetrapyrrolic structures were highlighted, having photodynamic therapy of cancer as a targeted biomedical application. Elemental analysis, NMR, FTIR and UV-Vis data in various solvents were provided. A preliminary in vitro study on normal and cancer cultured cells was carried out for biocompatibility assessment in dark conditions. The preliminary in vitro study performed on human peripheral mononuclear cells exposed to tetrapyrrolic compounds (2 μM) showed that the proposed compounds had a convenient cytotoxic profile on human normal peripheral blood mononuclear cells under dark conditions. Meanwhile, the investigated compounds reduced the number of metabolically active breast tumor MCF-7 cells, with the exception of Zn(II) complex-containing a symmetrical ligand. Accordingly, preliminary in vitro data suggest that the proposed tetrapyrrolic compounds are good candidates for PDT, as they limit tumor expansion even under dark conditions, whilst sparing normal cells. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Vieira Ferreira L.F.,University of Lisbon | Ferreira D.P.,University of Lisbon | Oliveira A.S.,University of Lisbon | Oliveira A.S.,Polytechnic Institute of Portalegre | And 5 more authors.
Dyes and Pigments | Year: 2012

A series of some A 3B type mesoporphyrinic compounds were synthesized with superior yields using the microwave irradiation. The prepared compounds were characterized by elemental analysis, FT-IR, UV-Vis, NMR and EPR spectroscopy, which fully confirmed their structures. The spectral molecular absorption properties of the porphyrinic compounds were studied in different solvents and the influence of the solvent polarity on the absorbance maxima was described. Fluorescence emission and singlet oxygen formation quantum yields were evaluated for A 3B type mesoporphyrinic compounds and compared with the corresponding symmetrical compounds, revealing high yields for the metal free compounds, followed by the zinc derivatives. The copper mesoporphyrinic compounds are not emissive and only evidence residual capacity for singlet oxygen formation. In order to establish their future potential in biomedical application preliminary toxicological studies, consisting of viability and proliferation of living cells in the presence of the unsymmetrical mesoporphyrinic compounds, were performed in the dark, on a standard cell line of human Caucasian histiocytic lymphoma (U937). The obtained results indicate a very low or no cytotoxicity at all for all compounds under study. Therefore further testing with light activation protocols is recommended in future work. © 2012 Elsevier Ltd. All rights reserved.


Socoteanu R.,Ilie Murgulescu Institute of Physical Chemistry | Anastasescu M.,Ilie Murgulescu Institute of Physical Chemistry | Oliveira A.,Polytechnic Institute of Coimbra | Oliveira A.,University of Lisbon | And 3 more authors.
International Journal of Photoenergy | Year: 2015

Fractal analysis of free bases porphyrins was computed on atomic force microscopy (AFM) micrographs using two different methods: the correlation function method and the variable length scale method. The correlation function method provides fractal dimension only for short scale range; results indicate that only few images have fractal properties for short ranges; for the rest of them, no fractal dimension was found using the correlation function method. The variable length scale method occur information for long range scaling. All samples have fractal properties at higher scaling range. For three samples the correlation function method leads to the same fractal dimension as the variable length scale method and scaling ranges for both methods overlap. Results show the necessity to use both methods to describe the fractal properties of AB3 meso-porphyrins that may be used to predict their relative cell localization. In order to emphasize the influence of fractal and textural properties the results regarding their self-similarity and texture/morphology were further compared with their behavior in biological assessment, that is, functionality of some Jurkat cell lines. © 2015 Radu Socoteanu et al.


PubMed | Victor Babes National Institute for Pathology and Biomedical science, Carol Davila University of Medicine and Pharmacy, University of Lisbon and Ilie Murgulescu Institute of Physical Chemistry
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2015

In this paper, two tetrapyrrolic complexes, Zn(II)-5-(3-hydroxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl)porphyrin and Cu(II)-5-(3-hydroxyphenyl)-10,15,20-tris-(4-acetoxy-3-methoxyphenyl)porphyrin were synthesized, and characterized from a spectral and biological point of view. The study provided data concerning the behavior of identical external substituents vs. two different core insertions. Some of the properties of the proposed tetrapyrrolic structures were highlighted, having photodynamic therapy of cancer as a targeted biomedical application. Elemental analysis, NMR, FTIR and UV-Vis data in various solvents were provided. A preliminary in vitro study on normal and cancer cultured cells was carried out for biocompatibility assessment in dark conditions. The preliminary in vitro study performed on human peripheral mononuclear cells exposed to tetrapyrrolic compounds (2 M) showed that the proposed compounds had a convenient cytotoxic profile on human normal peripheral blood mononuclear cells under dark conditions. Meanwhile, the investigated compounds reduced the number of metabolically active breast tumor MCF-7 cells, with the exception of Zn(II) complex-containing a symmetrical ligand. Accordingly, preliminary in vitro data suggest that the proposed tetrapyrrolic compounds are good candidates for PDT, as they limit tumor expansion even under dark conditions, whilst sparing normal cells.


Margina D.,Carol Davila University of Medicine and Pharmacy | Ilie M.,Carol Davila University of Medicine and Pharmacy | Manda G.,Victor Babes National Institute for Pathology and Biomedical science | Neagoe I.,Victor Babes National Institute for Pathology and Biomedical science | And 4 more authors.
General Physiology and Biophysics | Year: 2012

Quercetin and epigallocatechin gallate are two of the most abundant polyphenols in dietary plants, including apples, onions, red wine and green tea. The bioactivity of polyphenols is linked to their ability to interact with cell membranes without being internalized. The aim of the present study was to assess the short-time effect of these polyphenols on membrane anisotropy and transmembrane potential of U937 monocytes and Jurkat T lymphoblasts. Results showed that quercetin and epigallocatechin gallate induced, after 20 minutes cell exposure, a dose-dependent increase of membrane anisotropy and polarization. Anisotropy increase was correlated with the reduction of lipid peroxidation. Our results could indicate that the antioxidant capacity of the tested polyphenols is due to their stabilizing effect on the cell membranes, thus contributing to cell protection in various pathologies and as adjuvant therapy in highly toxic treatment regimens.


PubMed | Victor Babes National Institute for Pathology and Biomedical science
Type: Journal Article | Journal: Romanian journal of internal medicine = Revue roumaine de medecine interne | Year: 2010

No other medical field but nephrology showed so important achievements as a result of concerted efforts of doctors, biologists and technicians. Considering that in renal insufficiency, regardless of its aetiology, the common path is represented by the transitory or definite damage of the renal filter, many attempts have been made in order to reproduce the process of blood cleaning by the kidney.The high prevalence of cardiovascular diseases in hemodialysed population suggested that the disease could begin before or during the stage of chronic renal insufficiency. We investigated the vascular lesions, especially the immunologic features of patients involved in hemodialysis programs. Our study reflects a general picture of the immunologic status of hemodialysed patients, helping to understand the special profile ofa hemodialysed patient.Two groups have been selected and analyzed: one of 15 patients from the Hemodialysis Department in Sfntul Ioan Hospital, and another one composed of 30 patients with other diseases (the control group), from the Medical Department. The detection of specific antibodies against some HCV proteins corresponding to the most conserved regions of the viral genome has been done using the immune test LiaTEK HCV III. In the hemodialysed group, a blood sample has been drawn before and after the hemodialysis session, at 15-20 minutes, while in the other group the blood sample has been drawn together with the other tests. A flow cytometry examination was made at the Center of Immunology, in order to determine simultaneously several physical and chemical parameters. We analyzed the two groups of patients (HD/n=15; the reference group/n=30) regarding immunophenotyping, all types of lymphocytes and interleukin 2 (IL-2).The results have classified the HD patients into three subgroups, depending on the mean of the results from flow cytometry exam, referred to normal values. The assessment of the patients with or without HD to each group was made on the basis of the similar behavior of the markers investigated. The most affected age group in patients with HD was 31-40 years, followed by the age group 41-50 years (26%). The majority patients of the control group were of the same age 31-40 years old (40%), while 33% of them were between 41 and 50 years old. One subgroup (A) of HD patients showed the improvement of the total number of T lymphocytes (CD3+/CD19-) after the session, while the total number of B lymphocytes was stable. The number ofT lymphocytes with receptors for interleukin 2 (CD25) improved after hemodialysis. The second group (B) presented from the beginning a low number of total T lymphocytes (CD3+/CD19-). We found that the value of B lymphocytes (CD19+/CD3-) decreased after hemodialysis. Activated T lymphocytes (CD25), with receptors for interleukin 2, achieved greater values (3.66%), which cannot be found in the other groups. The third group (C) showed normal values for total T lymphocytes (CD3+/CD19-) before HD, which did not modify significantly after the session. The patients had the same decreased values for B lymphocytes, which have continued to decrease after the HD (7.98%).Post dialytic immunologic changes of the mononuclear cells represent the hallmark of the complexity of the immune response generally and especially too, in hemodialysed patients. We have noticed patients that presented an increase of the total number of T lymphocytes after the dialysis, but only T and NK lymphocytes and not B lymphocytes as well, suggesting the susceptibility to infections. The evaluation of the immune response using flow cytometry has confirmed the presence of high variations of the immune profile in hemodialysed patients, the decrease of T-cells activation but, it does not support the data regarding intrinsic functions of T and B cells. The high diversity of the immune response in hemodialysed patients is a consequence of the genetic individuality of each patient and also of the associated pathology or equipment used (viral infection, membrane type).

Loading Victor Babes National Institute for Pathology and Biomedical science collaborators
Loading Victor Babes National Institute for Pathology and Biomedical science collaborators