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Bucharest, Romania

Tanase C.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Cocu F.G.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Caproiu M.T.,Romanian Academy of Sciences | Draghici C.,Romanian Academy of Sciences | And 3 more authors.
Revista de Chimie | Year: 2010

Novel O2 -pyrimidine nucleosides with a functionalizedbicyclo[2. 2.1]heptan sugar moiety were synthesized startingfrom a pure optically active alcohol (4). This alcohol was coupled by Mitsunobu reaction to pyrimidine bases: N4-benzoylcytosine, 5-fluorouracil and N3-benzoyl- thymine, then the ester protected nucleosides were debenzoylated to the coresponding carbocyclic nucleosides, which looks to be Winked compounds. The compounds were characterized by IR, MS, 1H-NMR and 13C-NMR spectra. The preliminary in vitro preclinical results show that ent-27-5-FU (13) and ent-27-T (19) present cytotoxic or cytototxic activity in Jurkat lymphoblasts and/or U937 monocytic blasts.

Dobrea C.,Victor Babes National Institute | Al Salamat H.,University of Bucharest | Al Salamat H.,Alsamed Medical Center | Lupu A.R.,University of Bucharest
Revista Romana de Medicina de Laborator | Year: 2012

Diffuse large B-cell lymphoma (DLBCL) accounts for about 30% of non-Hodgkin's lymphoma (NHL) cases. The 2008 WHO Classification of Lymphoid Neoplasms recognizes several clinicopathological variants, subtypes and distinct disease entities of DLBCL. We present a review of clinical, pathological and molecular factors with implication in DLBCL behavior. Even if the golden standard therapy for CD20-positive DLBCL is still represented by R-CHOP, prognostic factor assessment could open new therapeutic perspectives.

Nicolae I.,Dermatovenereological Research Center | Nicolae C.D.,Carol Davila University of Medicine and Pharmacy | Coman O.A.,Dermatovenereological Research Center | Coman O.A.,Carol Davila University of Medicine and Pharmacy | And 3 more authors.
Romanian Journal of Morphology and Embryology | Year: 2011

Purpose: The gangliosides overexpression contributes to the development of skin melanoma. The purpose of this study was to determine if the total gangliosides serum levels might predict the tumor growth in patients with melanoma or if the transfer of shed cell gangliosides reflects the implication in the clinical prognostic of these patients. Patients and Methods: Total gangliosides serum levels were measured in the cryopreserved serum by estimating lipid-associated sialic acid in 761 patients before surgical resection of melanoma, in 406 patients with precancerous pigmentary lesions, and in 410 healthy individuals. This study was performed at the Dermatovenereological Research Center, Bucharest, Romania, during 1991-2010. All sera obtained after surgical resection of melanocytic tumors were analyzed to see if adjuvant therapy (chemo-, immuno-, immunochemo-therapy) induced gangliosides changes in melanoma patients and if the responses were correlated with survival. Results: Total gangliosides serum levels were higher in melanoma patients than in precancerous melanocytic lesions patients or in healthy individuals. Larger tumors in Breslow index and more advanced stage of disease were correlated with higher total gangliosides serum values. Augmented total gangliosides serum levels after melanoma adjuvant treatment were predictive for decreased overall survival, whereas decreased total gangliosides serum levels were predictable for improved overall survival. Conclusions: A marker for early melanoma complications and survival may be the total gangliosides serum level.

Ghita C.,Dr. Alexandru Gafencu Emergency Military Hospital | Vilcea I.D.,University of Medicine and Pharmacy of Craiova | Dumitrescu M.,University of Bucharest | Vilcea A.M.,University of Medicine and Pharmacy of Craiova | And 3 more authors.
Romanian Journal of Morphology and Embryology | Year: 2012

This study aim was to identify the relations between tumor suppressor genes (p53, bcl-2, PTEN), nuclear proliferative antigen Ki-67 and epidemiologic, morphologic and histologic patient related factors, in colorectal cancer. Materials and Methods: Twenty-two prospectively collected colorectal cancer resection specimens were histologically prepared, using standard paraffin-embedded and Hematoxylin-Eosin staining method; for immunohistochemical study, the Streptavidin-Biotin (sABC)/Horseradish Peroxidase (HRP) method was used. Results: P53 was positive in 86.36% of cases, more intense (>50%) in rectal cancer and in women 59.16±9.49-year-old; the G1/2 adenocarcinoma was dominant. Bcl-2 was positive in 18.18% of the cases, in distal colorectal cancer, only in men, 63.5±13.2-year-old. PTEN was positive in 95.45% of the cases; intense positivity was recorded in 12 men and six women, 61.47±11.67-year-old, in rectal topography. Ki-67 was positive in 86.36% of the cases, more intense in four men and four women, 63.45±12.22-year-old, in proximal and advanced colorectal cancers (pT3N1/2 - 62.5%). Conclusions: Tumor suppressor genes mutations are often present in colorectal cancer; the intensity of the expression of these mutations varies, which could explain the different prognosis for these patients.

Surlin V.,University of Medicine and Pharmacy of Craiova | Georgescu E.,University of Medicine and Pharmacy of Craiova | Dumitrescu C.,Medical Center | Ramboiu S.,University of Medicine and Pharmacy of Craiova | And 2 more authors.
Romanian Journal of Morphology and Embryology | Year: 2011

Cystic lymphangiomas are benign tumors developing as an anomaly of the lymphatic channels. Most of them are discovered in children and are located mostly in the head and neck with no differences between sexes. Retroperitoneal locations are rare, clinical symptoms not specific and are discovered incidentally. We report the case of a 32-year-old female sex patient with a cystic tumor situated in the retroperitoneum, behind the pancreas, discovered by abdominal ultrasound during a diagnostic work-out for urinary infection and hypertension. Anamnesis, physical examination and CT-scan could not indicate the nature of the cyst. The cystic tumor had a shape of a butterfly, crossing the midline in front of the aorta and vena cava. A laparotomy was indicated and established the diagnosis of a cystic lymphagioma based on its macroscopic appearance. The tumor was approached from its right side by a generous Kocher maneuver and then from the left by disinsertion of the Treitz ligament and elevating the pancreatic body and splenic vessels. It was completely removed. Postoperative course was uneventful. Histopathologic examination confirmed the diagnosis of benign cystic lymphangioma. At 2 years of follow-up the patient was free of recidive. Conclusions: Retropancreatic cystic lymphangioma are rarely seen in adults. Precise diagnosis is made at laparotomy, imagistic test give details about walls, content, relationship with the surrounding structures and major blood vessels. Complete surgical resection represents the treatment of choice avoiding recurrence.

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