Victor Babes National Institute

Bucharest, Romania

Victor Babes National Institute

Bucharest, Romania

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Pop O.T.,Research Center for Microscopic Morphology and Immunology | Cotoi C.G.,University of Medicine and Pharmacy of Craiova | Plesea I.E.,University of Medicine and Pharmacy of Craiova | Gherghiceanu M.,Victor Babes National Institute | And 4 more authors.
Romanian Journal of Morphology and Embryology | Year: 2011

The authors evaluated possible morphological changes of basement membrane (BM) and lamina propria (LP) of seminferous tubule wall (ST) related to ageing. Surgical samples of testicular tissue from 28 cases with orchiectomy for prostate adenocarcinoma were processed for light microscopy and transmission electron microscopy (TEM) examination. Seven age groups (AgGr) between 50 and 80 years were designed. Tissue samples were immunomarked for collagen IV and smooth muscle actin. Images were acquired and measured with a specialized software. Thirty ST were randomly selected, with ×40-objective, for each case. Five random determinations for each ST and each parameter were performed. Mean values/tubule, case and AgGr were calculated for each parameter. Regression line (RL), slope and significance test for slope were determined for each parameter correlation with ageing. BM mean value was around 0.5 μm, with narrow limits of ranging in AgGr but more extended individual limits. RL showed discrete decreasing trend with ageing but without an obvious statistical correlation. LP mean value was around 6 μm, also with narrow limits of ranging in AgGr and more extended individual limits. RL decreased discretely with ageing but without an obvious statistical correlation. TEM showed more prominent BM material and more collagen fibers and less fibroblasts in LP of older AgGr and higher fibroblasts density in LP of younger AgGr. Our results showed that BM thickness is apparently decreasing with ageing whereas LP presents extremely variable degenerative changes, with a "mosaic", focal distribution and no tendency to advance with ageing.


Ghita C.,Dr Alexandru Gafencu Emergency Military Hospital | Vilcea I.D.,University of Medicine and Pharmacy of Craiova | Dumitrescu M.,University of Bucharest | Vilcea A.M.,University of Medicine and Pharmacy of Craiova | And 3 more authors.
Romanian Journal of Morphology and Embryology | Year: 2012

This study aim was to identify the relations between tumor suppressor genes (p53, bcl-2, PTEN), nuclear proliferative antigen Ki-67 and epidemiologic, morphologic and histologic patient related factors, in colorectal cancer. Materials and Methods: Twenty-two prospectively collected colorectal cancer resection specimens were histologically prepared, using standard paraffin-embedded and Hematoxylin-Eosin staining method; for immunohistochemical study, the Streptavidin-Biotin (sABC)/Horseradish Peroxidase (HRP) method was used. Results: P53 was positive in 86.36% of cases, more intense (>50%) in rectal cancer and in women 59.16±9.49-year-old; the G1/2 adenocarcinoma was dominant. Bcl-2 was positive in 18.18% of the cases, in distal colorectal cancer, only in men, 63.5±13.2-year-old. PTEN was positive in 95.45% of the cases; intense positivity was recorded in 12 men and six women, 61.47±11.67-year-old, in rectal topography. Ki-67 was positive in 86.36% of the cases, more intense in four men and four women, 63.45±12.22-year-old, in proximal and advanced colorectal cancers (pT3N1/2 - 62.5%). Conclusions: Tumor suppressor genes mutations are often present in colorectal cancer; the intensity of the expression of these mutations varies, which could explain the different prognosis for these patients.


Dobrea C.,Victor Babes National Institute | Al Salamat H.,University of Bucharest | Al Salamat H.,Alsamed Medical Center | Lupu A.R.,University of Bucharest | Lupu A.R.,Colea Hospital
Revista Romana de Medicina de Laborator | Year: 2012

Diffuse large B-cell lymphoma (DLBCL) accounts for about 30% of non-Hodgkin's lymphoma (NHL) cases. The 2008 WHO Classification of Lymphoid Neoplasms recognizes several clinicopathological variants, subtypes and distinct disease entities of DLBCL. We present a review of clinical, pathological and molecular factors with implication in DLBCL behavior. Even if the golden standard therapy for CD20-positive DLBCL is still represented by R-CHOP, prognostic factor assessment could open new therapeutic perspectives.


Tampa M.,Carol Davila University of Medicine and Pharmacy | Matei C.,Carol Davila University of Medicine and Pharmacy | Popescu S.,Carol Davila University of Medicine and Pharmacy | Georgescu S.-R.,Carol Davila University of Medicine and Pharmacy | And 3 more authors.
Revista de Chimie | Year: 2013

Photodynamic therapy is an alternative antitumoral procedure that requires the use of a photosensitiser and a light source emitting radiation with an adequate wavelength, suitable for activating the photosensitiser; the most common photosensitisers are porphyrins and porphyrin-related substances. Phthalocyanines are macrocyclic chemical compounds structurally related to the porphyrins, as they both feature four linked pyrrole-like subunits, therefore possessing the potential of acting as good photosensitisers; metallo-substituted sulphonated phthalocyanines are novel chemical compounds that revealed promising results when used in PDT. Activated photosensitisers can lead to the destruction of the tumoral cells through production of reactive oxygen species and the induction of apoptosis. The present study aims to assess, via protein microarray analysis, the main molecular pathways involved in the apoptosis of dysplastic oral keratinocytes following photodynamic therapy using zinc trisulphonated phthalocyanine.


Tanase C.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Caproiu M.T.,Romanian Academy of Sciences | Draghici C.,Romanian Academy of Sciences | Cocu F.G.,Romanian National Institute for Chemical Pharmaceutical Research and Development | And 2 more authors.
Revista de Chimie | Year: 2011

By Mitsunobu reaction of N3-benzoyl 5-fluorouracil with 2-chloro-5-hydroxy-bicyclo[2.2.IJhept-7-ylmethyl benzoate no nucleoside was obtained. Instead nucleoside we observed a benzoate group migration with the formation of bis-benzoylated compound (5). The structure of this compound was proved by MS, IR, 1Hand 13C-NMR (with complementary COSY and HETCOR spectra), and also by synthesis of the compound by benzoylation of the starting intermediate (1).


Tanase C.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Cocu F.G.,Romanian National Institute for Chemical Pharmaceutical Research and Development | Caproiu M.T.,Romanian Academy of Sciences | Draghici C.,Romanian Academy of Sciences | And 3 more authors.
Revista de Chimie | Year: 2010

Novel O2 -pyrimidine nucleosides with a functionalizedbicyclo[2. 2.1]heptan sugar moiety were synthesized startingfrom a pure optically active alcohol (4). This alcohol was coupled by Mitsunobu reaction to pyrimidine bases: N4-benzoylcytosine, 5-fluorouracil and N3-benzoyl- thymine, then the ester protected nucleosides were debenzoylated to the coresponding carbocyclic nucleosides, which looks to be Winked compounds. The compounds were characterized by IR, MS, 1H-NMR and 13C-NMR spectra. The preliminary in vitro preclinical results show that ent-27-5-FU (13) and ent-27-T (19) present cytotoxic or cytototxic activity in Jurkat lymphoblasts and/or U937 monocytic blasts.


Laky D.,Victor Babes National Institute | Laky D.,Cardiovascular Institute | Laky D.,Semmelweis University | Parascan L.,Cardiovascular Institute | Candea V.,Cardiovascular Institute
Romanian Journal of Morphology and Embryology | Year: 2011

Arrhythmia's atrium fibrillation (AF) is the most often met in clinical setting and it is associated with an increased in mortality risk. For profound the structural changes in chronic AF, we are studied the morphological changes of atrium biopsies to be effected at 175 patients. With sustained AF malformative and valvular acquired cardiac diseases operated under extracorporeal circulation. Similar studies we are effected to 11 dogs with partial coronary obstructions to a made periodical EKG investigations. The morphological changes mainly concern accommodation (dedifferentiation) of cardiomyocytes (particularly at experimental model) and mal-accommodation (degeneration of cells with fibrosis replacement features) particularly in acquired valvular diseases. These changes were often interfered. Over study, maintain the hypothesis that the structural changes to be an accommodation more than degenerative response to AF.


Nicolae I.,Scarlat Longhin Hospital | Nicolae C.D.,Carol Davila University of Medicine and Pharmacy | Coman O.A.,Scarlat Longhin Hospital | Coman O.A.,Carol Davila University of Medicine and Pharmacy | And 3 more authors.
Romanian Journal of Morphology and Embryology | Year: 2011

Purpose: The gangliosides overexpression contributes to the development of skin melanoma. The purpose of this study was to determine if the total gangliosides serum levels might predict the tumor growth in patients with melanoma or if the transfer of shed cell gangliosides reflects the implication in the clinical prognostic of these patients. Patients and Methods: Total gangliosides serum levels were measured in the cryopreserved serum by estimating lipid-associated sialic acid in 761 patients before surgical resection of melanoma, in 406 patients with precancerous pigmentary lesions, and in 410 healthy individuals. This study was performed at the Dermatovenereological Research Center, Bucharest, Romania, during 1991-2010. All sera obtained after surgical resection of melanocytic tumors were analyzed to see if adjuvant therapy (chemo-, immuno-, immunochemo-therapy) induced gangliosides changes in melanoma patients and if the responses were correlated with survival. Results: Total gangliosides serum levels were higher in melanoma patients than in precancerous melanocytic lesions patients or in healthy individuals. Larger tumors in Breslow index and more advanced stage of disease were correlated with higher total gangliosides serum values. Augmented total gangliosides serum levels after melanoma adjuvant treatment were predictive for decreased overall survival, whereas decreased total gangliosides serum levels were predictable for improved overall survival. Conclusions: A marker for early melanoma complications and survival may be the total gangliosides serum level.


PubMed | Victor Babes National Institute
Type: Journal Article | Journal: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie | Year: 2012

Gastrointestinal stromal tumors (GISTs) are the most common primary mesenchymal neoplasias of the gastrointestinal tract, typically expressing c-kit (CD117) and CD34. Recently, it was reported that nestin and caveolin-1 are also expressed in some human sarcomas, GISTs included. We performed a retrospective study on formalin fixed, paraffin embedded samples from 81 cases of confirmed GISTs, aiming to characterize their immunohistochemical profile, including nestin and caveolin-1 expressions. Tissue samples were evaluated immunohistochemically for CD117, CD34, nestin and caveolin-1. The patients (M:F 36:45), aged 46 to 84 years, had spindle cell type GISTs in 56.7% of cases, epithelioid in 30.8% and mixed pattern in 12.3%. Immunohistochemically, CD117 was positive in 88.9% of GISTs, CD34 in 85.1%, nestin in 77.7% and caveolin-1 in 71.6% of the tumors. Of nine c-kit negative GISTs, 66.7% expressed nestin, the same as caveolin-1 and 44.5% expressed both nestin and caveolin-1. Statistical analysis using Kendalls and Spearmans tests revealed significant correlations between nestin and caveolin-1 expressions (p=0.024). Our results suggest that nestin and caveolin-1 could be considered sensitive markers in GISTs, together with CD117 and CD34, for diagnostic purposes. Their significant expression in CD117 negative GISTs could represent an immunohistochemical alternative in establishing the diagnosis of these tumors.


PubMed | Victor Babes National Institute
Type: Case Reports | Journal: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie | Year: 2012

We report a rare case of renal tumor--mucinous tubular and spindle cell carcinoma in a 65-year-old man. The tumor, located in the right kidney, was well circumscribed. Microscopically, the tumoral proliferation was composed of cuboidal cells arranged in tubules, with abrupt transition to spindle cell morphology in a myxoid stroma. Because of the favorable prognosis with this type of tumor, mucinous tubular and spindle cell carcinoma must be differentiated from papillary renal cell carcinoma, especially the variant with sarcomatoid dedifferentiation.

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