Pereira D.A.A.,Faculdades Sudamerica |
Vieira S.A.,Federal University of Vicosa |
Fogal A.S.,Vicosa |
Ribeiro A.Q.,Federal University of Vicosa |
Franceschini S.C.C.,Federal University of Vicosa
Revista de Nutricao | Year: 2014
This text aims to foster the reflection and criticism in the process of developing research projects in clinical nutrition. We present aspects regarding the evidence, validity, and reliability of results of studies in this field. Appropriate study planning is critical, from defining the design and type of experiment, going through the ethical aspects, population choice, and calculation of sample size, to the assessment of the feasibility of the risks involved in study execution. Once the information is collected, the next stages correspond to the description of the results, statistical analyses, verification of the consistency of these results, and ultimately their correct interpretation.
Characteristics of thawed goat semen after addition of ringer lactate, sodium citrate and tris solution [Características do sêmen caprino descongelado após a adição de ringer lactato, citrato de sódio e solução tris]
Dias J.C.O.,Vicosa |
Santos M.C.R.,Vicosa |
Filho J.M.P.,Vicosa |
Oliveira G.D.,Vicosa |
And 2 more authors.
Ciencia Animal Brasileira | Year: 2015
Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology.