Characteristics of thawed goat semen after addition of ringer lactate, sodium citrate and tris solution [Características do sêmen caprino descongelado após a adição de ringer lactato, citrato de sódio e solução tris]
Dias J.C.O.,Vicosa |
Santos M.C.R.,Vicosa |
Filho J.M.P.,Vicosa |
Oliveira G.D.,Vicosa |
And 2 more authors.
Ciencia Animal Brasileira | Year: 2015
Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology.
Pereira D.A.A.,Faculdades Sudamerica |
Vieira S.A.,Federal University of Viçosa |
Fogal A.S.,Vicosa |
Ribeiro A.Q.,Federal University of Viçosa |
Franceschini S.C.C.,Federal University of Viçosa
Revista de Nutricao | Year: 2014
This text aims to foster the reflection and criticism in the process of developing research projects in clinical nutrition. We present aspects regarding the evidence, validity, and reliability of results of studies in this field. Appropriate study planning is critical, from defining the design and type of experiment, going through the ethical aspects, population choice, and calculation of sample size, to the assessment of the feasibility of the risks involved in study execution. Once the information is collected, the next stages correspond to the description of the results, statistical analyses, verification of the consistency of these results, and ultimately their correct interpretation.
PubMed | Vicosa
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015
The objective of this study was to select genitors based on F1 and F2 generations, evaluated in different environments, to obtain segregating populations for the identification of strains showing improved earliness, yield, and carioca-type grains. Nine bean strains were crossed in a partial diallel scheme (4 x 5), in which group 1 included 4 strains with early cycles and group 2 included 5 elite strains. The F1 and F2 generations and the genitors were assessed in Coimbra and Viosa in randomized blocks with 3 replications. The following characteristics were evaluated: days between sowing and emergence, and grain yield. We observed an interaction between the effects of general combining ability and specific combining ability with the environments (crop, location, and generation) for both grain earliness and yield. Genetic analysis of earliness revealed a predominance of additive effects and grain yield dominance effects. The strain Goiano Precoce may be used as a genitor in breeding programs to improve earliness, while strains RP1 and VC33 can be used to increase grain yield. We observed genetic complementation between strains Goiano Precoce and RP1, BRSMG Madreprola and BRS Estilo for earliness and between RP1 and Rosinha Precoce for grain yield.