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Popovača, Croatia

Zobel R.,Vetmed doo | Tkalcic S.,Western University of Health Sciences | Stokovic I.,University of Zagreb | Pipal I.,Vetmed doo | Buic V.,Vetmed doo
Reproduction in Domestic Animals

Contents: Urovagina is detrimental to the health and fertility of cows worldwide. Surgery, the only known treatment, is not economically pragmatic to dairy practice, and it has some medical drawbacks. The objective of this study was to determine whether reproductive problems associated with urovagina could be alleviated through more practical and less-invasive treatment options to allow successful conception and term-pregnancy. Cows diagnosed with urovagina (n=1219) were divided into three treatment groups, each containing an equal number of cows with mild, moderate and severe urovagina. The groups received one of three treatment options: saline (group A, n=400), streptomycin (group B, n=400) or ozone (group C, n=419) flush, regardless of the severity of the urovagina condition within the group. The ozone treatment was found to be the most effective treatment modality, resulting in the shortest period of days open (95, 89 and 79days in groups A, B and C, respectively; p<0.05), the fewest number of inseminations until pregnancy (2.38, 1.84 and 1.63 in groups A, B and C, respectively; p<0.05) and the smallest number of culled cows (20, 23 and 12 in groups A, B and C, respectively; p<0.05). The ozone flush coupled with intracornual insemination presents an effective treatment option for urovagina that can lead to successful conceptions and pregnancies in dairy cows. © 2011 Blackwell Verlag GmbH. Source

Zobel R.,Vetmed doo | Geres D.,University of Zagreb | Pipal I.,Vetmed doo | Buic V.,Vetmed doo | And 2 more authors.
Reproduction in Domestic Animals

Contents: It has been suggested that the time of insemination has effect on the calves' sex ratio because of the differences in timing of capacitation, motility and survival time of the X and Y spermatozoa in the female reproductive tract. We have conducted a field trial to study the effects of different semen deposition sites on the sex ratio and fertility in cattle. Two groups of 450 cows were inseminated via artificial insemination: group A was inseminated into the uterine body and group B was inseminated deep into the uterine horn ipsilateral to the ovary with dominant follicle. After applying several exclusion criteria, a total of 607 pregnant cows were considered for data analysis (group A=318 and group B=289 cows). The conception rate was 7% higher (p<0.05) in the group A, with 23% more of the male calf pregnancies (p<0.001). At the same time, 18% more of the female calves were calved in the group B (p<0.005). The difference in male calves between the two groups was 21% and in female calves was 20% (p<0.001). We conclude that semen deposition site plays a significant role in differences in gender ratio observed after calving. Intracornual semen deposition resulted in a higher ratio of female calves, whereas uterine body deposition site resulted in higher male calves ratio, probably contributing physiologically to the differences in motility, capacitation time, the lifespan of X vs Y spermatozoa and to the pronounced shift of X spermatozoa in the female genital tract. © 2010 Blackwell Verlag GmbH. Source

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