Kirovski D.,University of Belgrade |
Blond B.,Veterinary Station |
Katic M.,Veterinary Station |
Markovic R.,University of Belgrade |
Sefer D.,University of Belgrade
Applied Adhesion Science | Year: 2015
Background: An addition of rumen-protected fat to the diet of cows may limit negative energy balance and/or shorten its duration, leading to increased milk production with reduced risk of metabolic disorders in dairy cows. The aim of the study was to test the effect of rumen-inert fat supplement of palm oil on milk production, milk composition, rumen characteristics, and metabolic variables of early lactating dairy cows. For this purpose, 24 Holstein-Friesian cows were divided into two equal groups and fed a corn silage-based diet, without palm oil supplementation (control) or with 300 g palm oil (Palm Fat 99, Noack & Co. GmbH, Vienna, Austria) per cow for 8 weeks starting from day 30 after parturition. Milk, rumen, and blood samples were taken three times during experiment at days 30, 58, and 86 of lactation. Body condition scores of cows were determined in the same time periods. Milk yields were measured at the morning and evening milking (600 and 1800). Milk samples were analyzed for milk fat and milk protein content. Rumen content was tested for electrochemical reaction. A native slide was prepared for microscopical examination of the rumen protozoa motility that was numerically estimated. Protozoa were counted in whole rumen contents by light microscopy. Blood samples were tested for total protein, albumin, urea, tryglicerides, cholesterol, total bilirubin, beta-hydroxybutyrate, Ca, and P. Results: Compared with the control, palm oil supplementation resulted in an increase of the average milk yield and milk fat content. The loss in body condition was significantly lower in the group fed palm oil than in the control group. Rumen pH, total number, and motility of protozoa in the group fed palm oil were significantly higher than those in the control group. Palm oil supplementation did not influence blood metabolite concentrations except for urea and glucose which were significantly lower and Ca and cholesterol which were significantly higher in the palm oil-supplemented group. Conclusions: Our results indicate that supplementation with palm oil in weeks 4 to 12 postpartum spared postpartum body weight loss, increased milk yield and milk fat content, and had positive effects on rumen characteristics. © 2015, Kirovski et al.; licensee Springer. All Right Reserved.
Samanc H.,University of Belgrade |
Danijela K.,University of Belgrade |
Stojic V.,University of Belgrade |
Dragica S.,Scientific Veterinary Institute Novi Sad |
And 3 more authors.
Acta Veterinaria | Year: 2011
The aim of this study was to examine the possibility of using results of metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows. Forty dry cows, 5 to 7 days before calving, were chosen from the commercial dairy herd and included in the study Four blood samples were taken by jugular venipuncture from each animal: 5 to 7 days before expected calving (dry period), as well as on day 12, 30 and 60 of lactation. Concentrations of glucose, β-hydroxybutyrate (BHBA), total protein, albumin, urea, total bilirubin, calcium and phosphorus concentrations were measured in all blood samples. On day 12 after calving, liver percutaneous biopsies were obtained using a biopsy instrument. Liver tissue lipid content was determined by pathohistological determination. Cows were divided into two groups of equal size based on the degree of lipid accumulation in the liver: healthy cows (0.00 fat, n = 20) and cows with fatty liver syndrome (>20% fat, n = 20). Milking was measured each day from day 7 to day 60 of lactation. Results showed that 5 to 7 days before calving, as well as 12 days after calving, BHBA concentrations were significantly higher (p<0.001, respectively) in diseased cows than in healthy cows. Additionally, the concentration of glucose was significantly lower (p<0.01) and the concentration of total bilirubin significantly higher (p<0.001) in diseased compared to healthy cows, at day 12 of lactation. Thirty days after calving, concentrations of albumin, glucose, BHBA and Ca were significantly lower in diseased compared to healthy cows (p<0.05, p<0.01, p<0.01 and p<0.001, respectively). At day 60 after calving, concentrations of biochemical parameters did not differ between diseased and healthy cows. The lactation curve differed in diseased compared to healthy cows, starting from day 26 of lactation. Starting form that day until day 60 of lactation average daily milk production was significantly higher in healthy than in diseased cows, except on day 32 of lactation when the difference was not significant. Based on these results it can be concluded that the metabolic profile test may be a reliable tool for the prediction and diagnosis of fatty liver in Holstein cows.
Samanc H.,University of Belgrade |
Danijela K.,University of Belgrade |
Jovanovic M.,University of Belgrade |
Vujanac I.,University of Belgrade |
And 4 more authors.
Acta Veterinaria | Year: 2010
The aim of this study was to estimate the association between body condition and fatty liver in high-yielding dairy cows. One hundred dry Holstein cows were selected. Cows were scored once for body condition during the dry period, Puerperium and month 2 of lactation, according to the system provided by Elanco Animal Health Buletin Al 8478. Body condition loss was determined as marked if loss was over 0.7 points between two consecutive phases of cycles and over 1.5 points between Puerperium and month 2 of lactation. Liver tissue samples were taken 12 d after calving and tested for lipid content. 50% of cows had mild (3.92 ± 3.33% fat), 33% moderate (19.28 ± 5.18% fat), and 17% severe fatty liver (36.21 ± 4.55% fat). The mean body condition scores were 3.79 ± 0.55 (dry period), 3.18 ± 0.34 (Puerperium) and 2.90 ± 0.29 (month 2 of lactation). Marked body condition loss from the dry period to Puerperium had 38% of cows, 20% from dry period to month 2 of lactation and 8% from Puerperium to month 2 of lactation. Body condition scores out of the physiological range had 39% dry cows, 49 % cows in puerperium and 9% cows at month 2 of lactation. After dividing cows into groups according to fatty liver degree, it was shown that only cows with severe fatty liver were obese during the dry period and that 76.47% of cows from this group had marked condition loss from the dry period to puerperium, 47.06% from dry period to month 2 of lactation and 23.53% from puerperium to month 2 of lactation. These indicate that in cows with severe fatty liver lipomobilisation is intensive and starts around calving, before milk production enhacement. Our results indicate a strong association between body condition and fatty liver in cows, which can be estimated only when body condition is analyzed in details. Besides, our results clearly indicate that body condition is not the only etiological factor that leads to fatty liver in dairy cows.
Shi M.,Chinese National Institute for Communicable Disease Control and Prevention |
Shi M.,University of Sydney |
Lin X.-D.,U.S. Center for Disease Control and Prevention |
Vasilakis N.,University of Texas Medical Branch |
And 15 more authors.
Journal of Virology | Year: 2016
Viruses of the family Flaviviridae are important pathogens of humans and other animals and are currently classified into four genera. To better understand their diversity, evolutionary history, and genomic flexibility, we used transcriptome sequencing (RNA-seq) to search for the viruses related to the Flaviviridae in a range of potential invertebrate and vertebrate hosts. Accordingly, we recovered the full genomes of five segmented jingmenviruses and 12 distant relatives of the known Flaviviridae ("flavilike" viruses) from a range of arthropod species. Although these viruses are highly divergent, they share a similar genomic plan and common ancestry with the Flaviviridae in the NS3 and NS5 regions. Remarkably, although these viruses fill in major gaps in the phylogenetic diversity of the Flaviviridae, genomic comparisons reveal important changes in genome structure, genome size, and replication/gene regulation strategy during evolutionary history. In addition, the wide diversity of flavi-like viruses found in invertebrates, as well as their deep phylogenetic positions, suggests that they may represent the ancestral forms from which the vertebrate-infecting viruses evolved. For the vertebrate viruses, we expanded the previously mammal-only pegivirus-hepacivirus group to include a virus from the graceful catshark (Proscyllium habereri), which in turn implies that these viruses possess a larger host range than is currently known. In sum, our data show that the Flaviviridae infect a far wider range of hosts and exhibit greater diversity in genome structure than previously anticipated. © 2015, American Society for Microbiology.
Kang Y.-J.,Chinese National Institute for Communicable Disease Control and Prevention |
Kang Y.-J.,Center for Diagnosis and Treatment of Infectious Diseases |
Diao X.-N.,Veterinary Station |
Zhao G.-Y.,Chinese National Institute for Communicable Disease Control and Prevention |
And 16 more authors.
BMC Evolutionary Biology | Year: 2014
Background: Bacteria of the order Rickettsiales (Alphaproteobacteria) are obligate intracellular parasites that infect species from virtually every major eukaryotic lineage. Several rickettsial genera harbor species that are significant emerging and re-emerging pathogens of humans. As species of Rickettsiales are associated with an extremely diverse host range, a better understanding of the historical associations between these bacteria and their hosts will provide important information on their evolutionary trajectories and, particularly, their potential emergence as pathogens. Results: Nine species of Rickettsiales (two in the genus Rickettsia, three in the genus Anaplasma, and four in the genus Ehrlichia) were identified in two species of hard ticks (Dermacentor nuttalli and Hyalomma asiaticum) from two geographic regions in Xinjiang through genetic analyses of 16S rRNA, gltA, and groEL gene sequences. Notably, two lineages of Ehrlichia and one lineage of Anaplasma were distinct from any known Rickettsiales, suggesting the presence of potentially novel species in ticks in Xinjiang. Our phylogenetic analyses revealed some topological differences between the phylogenies of the bacteria and their vectors, which led us to marginally reject a model of exclusive bacteria-vector co-divergence. Conclusions: Ticks are an important natural reservoir of many diverse species of Rickettsiales. In this work, we identified a single tick species that harbors multiple species of Rickettsiales, and uncovered extensive genetic diversity of these bacteria in two tick species from Xinjiang. Both bacteria-vector co-divergence and cross-species transmission appear to have played important roles in Rickettsiales evolution. © 2014 Kang et al.; licensee BioMed Central Ltd.
Mehlhorn H.,Heinrich Heine University Düsseldorf |
Schumacher B.,Veterinary Station |
Jatzlau A.,Veterinary Station |
Abdel-Ghaffar F.,Cairo University |
And 3 more authors.
Parasitology Research | Year: 2011
Ticks are known to be able to transmit a broad spectrum of agents of diseases in cattle or sheep. Therefore, measurements are needed to keep ticks away from the body of any ruminant belonging to the agricultural life stock. The present study dealt with investigations to measure the efficacy of the insecticide deltamethrin (Butox® 7.5 pour on) against specimens of two important species (Ixodes ricinus and Rhipicephalus sanguineus). Four sheep and four young cattle were treated lege arte along the vertebral column with 10 ml Butox® (deltamethrin) per sheep or 30 ml Butox® per cattle. Day 7, 14, 21, and 28 after the treatment, hair was shaved off from the head, ears, the back, belly, and the feet being collected in separate, suitable plastic bags, and transported to the institute, where these hair were brought into close contact with either adult and/or nymph stages of I. ricinus and R. sanguineus. As results, strong, acaricidal effects were seen, which varied according to the parasite species, the origin of the hair (e.g., head, leg, etc.) and according to the period after the treatment. In sheep, the acaricidal effect was noted for the whole period of 28 days along the whole body with respect to adults and nymphs of I. ricinus, while the acaricidal effects of deltamethrin were reduced for R. sanguineus stages beginning at day 21 after treatment. In cattle, the full acaricidal effect was seen for 21 days in I. ricinus stages and for 14 days in R. sanguineus, while the acaricidal efficacy became reduced after these periods of full action - beginning at the hair taken from the legs. Only R. sanguineus adults did not show any reaction on day 28 after treatment. Besides these acaricidal effects, repellent effects were also noted. Full repellency for both species was seen during the first 14 days in sheep and cattle against Ixodes and Rhipicephalus, while the repellency was later reduced, especially in contact with hair from the legs. As conclusion, deltamethrin, besides its very good effects against biting insects, brings acaricidal as well as repellent effects against ticks, thus protecting the sheep and cattle from transmission of agents of diseases. © Springer-Verlag 2010.
PubMed | Guangxi Mangrove Research Center, Centers for Disease Control and Prevention, University of Texas Medical Branch, Chinese National Institute for Communicable Disease Control and Prevention and Veterinary Station
Type: Journal Article | Journal: Journal of virology | Year: 2015
Viruses of the family Flaviviridae are important pathogens of humans and other animals and are currently classified into four genera. To better understand their diversity, evolutionary history, and genomic flexibility, we used transcriptome sequencing (RNA-seq) to search for the viruses related to the Flaviviridae in a range of potential invertebrate and vertebrate hosts. Accordingly, we recovered the full genomes of five segmented jingmenviruses and 12 distant relatives of the known Flaviviridae (flavi-like viruses) from a range of arthropod species. Although these viruses are highly divergent, they share a similar genomic plan and common ancestry with the Flaviviridae in the NS3 and NS5 regions. Remarkably, although these viruses fill in major gaps in the phylogenetic diversity of the Flaviviridae, genomic comparisons reveal important changes in genome structure, genome size, and replication/gene regulation strategy during evolutionary history. In addition, the wide diversity of flavi-like viruses found in invertebrates, as well as their deep phylogenetic positions, suggests that they may represent the ancestral forms from which the vertebrate-infecting viruses evolved. For the vertebrate viruses, we expanded the previously mammal-only pegivirus-hepacivirus group to include a virus from the graceful catshark (Proscyllium habereri), which in turn implies that these viruses possess a larger host range than is currently known. In sum, our data show that the Flaviviridae infect a far wider range of hosts and exhibit greater diversity in genome structure than previously anticipated.The family Flaviviridae of RNA viruses contains several notorious human pathogens, including dengue virus, West Nile virus, and hepatitis C virus. To date, however, our understanding of the biodiversity and evolution of the Flaviviridae has largely been directed toward vertebrate hosts and their blood-feeding arthropod vectors. Therefore, we investigated an expanded group of potential arthropod and vertebrate host species that have generally been ignored by surveillance programs. Remarkably, these species contained diverse flaviviruses and related viruses that are characterized by major changes in genome size and genome structure, such that these traits are more flexible than previously thought. More generally, these data suggest that arthropods may be the ultimate reservoir of the Flaviviridae and related viruses, harboring considerable genetic and phenotypic diversity. In sum, this study revises the traditional view on the evolutionary history, host range, and genomic structures of a major group of RNA viruses.