Veterinary Science University of Madhya Pradesh

www.mppcvv.org
Surendranagar, India

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Qadir S.,Veterinary Science University of Madhya Pradesh | Dixit A.K.,Veterinary Science University of Madhya Pradesh | Dixit P.,Veterinary Science University of Madhya Pradesh
Journal of Helminthology | Year: 2011

The effect of canine intestinal helminths on the haematological profile of 200 dogs, of both sexes and variable age, visiting university veterinary clinics for routine examination was investigated. The dogs were assigned to parasitized (n=39) and non-parasitized (n=161) groups of animals. Coprological examination revealed a 19.5% prevalence of different species of the helminths. Of these animals, 10.25% had mixed infections with Ancylostoma caninum, Toxascaris spp. and Dipylidium caninum. The intensity of A. caninum infection was the highest, with mean egg counts of 951.43 (standard error 88.66), followed by Toxascaris 283.33 (standard error 116.81) and D. caninum. The parasitized animals had significantly lower levels of haemoglobin, packed cell volume and total erythrocyte counts than non-parasitized animals (P<0.01). Values of other parameters, except for lymphocytes and eosinophils, were not different between the two groups. Analyses of the haematological profile revealed normocytic hypochromic anaemia in the parasitized group of animals. © 2010 Cambridge University Press.


Gokhale M.,St Aloysius College Autonomous | Jain S.,Veterinary Science University of Madhya Pradesh
Toxicology International | Year: 2014

Context: To explore genotoxicity in bidi rollers occupationally exposed to bidi tobacco dust. Aims: To assess the extent of genotoxicity of tobacco dust to bidi rollers of Jabalpur, Madhya Pradesh, India and cytotoxicity of bidi tobacco extract. Settings and Design: Blood samples from 31 bidi rollers and 30 controls taken after written informed consent were analyzed for chromosome aberrations (CA) and comet assay. Materials and Methods: G enotoxicity was studied by CA in cultured peripheral blood lymphocytes of bidi rollers and the deoxyribonucleic acid (DNA) damage studies were done by comet assay of their blood. The toxicity of bidi tobacco extract to normal human lymphocytes was studied by MMT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay as drop in viability. Statistical Analysis Used: Student′s t-test and DMRT. Results: There is a general trend of increase in CA% of both in exposed and control groups with age, but in every group the bidi rollers have a significantly higher CA% than the controls. The CA % is also directly related to exposure. The comet assay findings reveal that the mean comet length and tail length increases with exposure time. The toxicity of bidi tobacco extract (TE) to normal human lymphocytes was tested in vitro by 3-[4,5-dimethylthiazol-2-yl]- 2,5 diphenyl tetrazolium bromide (MTT) assay at 2 h of incubation. The trend of drop in viability with increasing concentrations of TE was clearly evident from the data from four donors in spite of their individual differences in viability. Conclusions: The results obtained in this investigation indicate that bidi rollers seem to be facing the occupational hazard of genotoxicity due to handling bidi tobacco and inhalation of tobacco dust. They should be advised to work under well-ventilated conditions.


Chandra N.,Veterinary Science University of Madhya Pradesh | Jain N.K.,Veterinary Science University of Madhya Pradesh | Sondhia S.,Directorate of Weed Science Research | Srivastava A.B.,Veterinary Science University of Madhya Pradesh
Bulletin of Environmental Contamination and Toxicology | Year: 2013

Deltamethrin (DM) is a broad-spectrum insecticide mainly used to protect crops, fruit and vegetables from pests such as mites, ants, weevils and beetles. Birds, animals and human beings living in same ecosystem are directly or indirectly at the risk of exposure to this insecticide leading to substantial decrease in growth. Thus we studied DM induced toxicity and ameliorative effects of alpha-tocopherol in broiler birds. DM was estimated in liver, breast and leg muscles of chickens feeding with only DM or DM with alpha-tocopherol daily for 42 days. Birds exposed to DM showed a dose dependent decrease in body weight on 5th, 6th and 7th weeks as compared to controls, and alpha-tocopherol partially restored the reduction in body weight. DM residue was found higher in liver as compared to breast and leg muscles. © 2013 Springer Science+Business Media New York.


Kumar A.,Up Pt Deen Dayal Upadhyaya Veterinary Science University Duvasu | Roy B.,Veterinary Science University of Madhya Pradesh | Lakhani G.P.,Veterinary Science University of Madhya Pradesh | Jain A.,Veterinary Science University of Madhya Pradesh
Veterinary World | Year: 2014

Aim: The aim was to investigate the effect of bread waste feeding on feed intake, utilization and growth performance of crossbred pigs and to evaluate the economics of bread waste feeding. Materials and Methods: The study was conducted for a period of 3 months (December, 2012-March, 2013). Large White Yorkshire crosses (LWY X Desi) were included in the study. A total of 24 weaned crossbred growing pigs were randomly assigned to four different groups, with six animals in each group. The experimental animals were fed as per their body weight following Indian Council of Agricultural Research requirements. They were fed twice a day according to schedule at 9:00 am and 3:00 pm. The water was kept available to animals round the clock. The cost of the experimental diets was calculated based on the existing price of the ingredients during the experimental period. Samples of feed were collected for analysis of proximate principles as per Association of Official Analytical Chemist. Body weight of all animals was recorded fortnightly basis in the morning before feeding with the use of platform type electronic weighing balance. Feed conversion ratio (FCR) and protein conversion ration was calculated by using a standard formula. Data were analyzed, using one-way analysis of variance (ANOVA). Means showing significant differences in the ANOVA table were compared using the Duncan multiple range test. Results: Crude protein content (%) of the diets was 16.01±0.02, 16.13±0.02, 16.14±0.02, 16.04±0.02, 16.08±0.02 and 12.72±0.03, respectively for the G1, G2, G3 and G4, groups. Proximate compositions (%) of diets used in the experiment were not significantly different. Digestibility coefficients of all the nutrients were significantly varied among the experimental groups. Daily feed intake was significantly (p<0.05) varied among the groups. Average daily gain (kg/pig/day) were significantly (p<0.01) higher in group G2 (0.377). FCR was significantly (p<0.01) higher in the G2 group (4.35). Conclusion: Bread waste can economically supplement up to 50% to the diet of crossbred growing pigs. © The authors.


Ghosh S.,Veterinary Science University of Madhya Pradesh | Mehla R.K.,National Dairy Research Institute
Tropical Animal Health and Production | Year: 2012

Thirty-six Holstein cross calves 5 days of age in their preruminant stage were used to study the effect of feeding prebiotic (mannanoligosaccharide) on their performance up to the age of 2 months. Treatment and control groups consisted of 18 calves each. Treatment group was supplemented with 4 g prebiotic (mannanoligosaccharide)/calf/day. Performance was evaluated by measuring average body weight (BW) gain, feed intake [dry matter (DM), total digestible nutrient (TDN) and crude protein(CP)], feed conversion efficiency (DM, TDN, and CP), fecal score, fecal coliform count and feeding cost. Body weight measured weekly, feed intake measured twice daily, proximate analysis of feeds and fodders analyzed weekly, fecal score monitored daily and fecal coliform count done weekly. There was a significant increase in average body weight gain, feed intake and feed conversion efficiency; and a significant decrease in severity of scours as measured by fecal score and fecal coliform count in the treatment group compared with control group (P < 0.01). Feed cost/kg BW gain was significantly lower in the treatment group compared to control group (P < 0.01). The results suggest that prebiotic (mannanoligosaccharide) can be supplemented to the calves for better performance. © 2011 Springer Science+Business Media B.V.


Kumar D.,Veterinary Science University of Madhya Pradesh | Gopalakrishna R.,Veterinary Science University of Madhya Pradesh | Singh A.P.,Veterinary Science University of Madhya Pradesh | Ranjan R.,Veterinary Science University of Madhya Pradesh | And 2 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2014

The developmental potency of pre-implant parthenogentic goat embryos were compared under two chemical activation protocols in three different culture media groups. The in vitro matured oocytes were chemically activated by two protocols viz. P1 (CB-CHX-6DMAP) and P2 (Ca-CHX-6DMAP). The activated oocytes under both the protocols were developed in three culture media, viz. modified synthetic oviductal fluid (mSOF), research vitro cleave medium (RVCL), and RVCL-Blast. While comparing the developmental potential of activated oocytes, it was observed that the oocytes activated under P2 protocol pooled over three culture media group producing significantly higher mean cleavage rate (43.2 ± 0.9 vs 40.6 ± 1.5), blastocyst development (16.4 ± 1.1 vs 12.6 ± 0.8), and blastomere count (120.7 ± 4.7 vs 113.2 ± 4.1) as compared to P1 protocol. The comparison of effect of culture media pooled over protocol groups revealed that the mean cleavage rate observed under RVCL-Blast (44.8 ± 1.3) and RVCL (45.3 ± 0.5) were significantly higher (P ≤ 0.01) than mSOF (35.8 ± 1.2). However, the mean blastocyst development observed under RVCL-Blast group (18.8 ± 3.2) was significantly higher than RVCL (14.0 ± 0.8) and mSOF (10.8 ± 0.4). Similarly, the mean blastomere count under RVCL-Blast group (136.0 ± 3.7) was significantly higher (P ≤ 0.01) than RVCL (114.7 ± 1.0) and mSOF (100.2 ± 0.5) groups. The semiquantitative RT PCR analysis showed the expression of pro-apoptotic caspase 3 gene in P1 and anti-apoptotic Mcl-1 gene in P2. This study concludes that the activation protocol P2 and embryo cultured under RVCL-Blast group were optimum for chemical activation and culture medium, respectively. © 2013 The Society for In Vitro Biology.


Ghosh S.,Veterinary Science University of Madhya Pradesh | Mehla R.K.,National Dairy Research Institute | Sirohi S.K.,National Dairy Research Institute | Tomar S.K.,National Dairy Research Institute
Journal of Animal Physiology and Animal Nutrition | Year: 2011

Twelve crossbred calves (Holstein cross) in their pre-ruminant stage were used to study the effect of garlic extract feeding on their performance and they were randomly allotted into treatment and control groups in equal number. Performance was evaluated by measuring average body weight (BW) gain, feed intake [dry matter (DM); total digestible nutrient (TDN) and crude protein (CP)], feed conversion efficiency (DM, TDN and CP), fecal score and fecal coliform count. Diets were same for both groups. In addition, treatment group received garlic extract supplementation at 250mg/kg BW/day/calf. BW measured weekly, feed intake measured twice daily, proximate analysis of feeds and fodders analysed weekly, fecal scores monitored daily and fecal coliform count done weekly. There was a significant (p<0.01) increase in mean BW gain and feed intake and a significant (p<0.01) decrease in severity of scours as measured by fecal score in the treatment group compared to the control group. The results suggest that garlic extract can be supplemented to the calves for better performance. © 2010 Blackwell Verlag GmbH.


Singh N.K.,Veterinary Science University of Madhya Pradesh | Singh S.,Veterinary Science University of Madhya Pradesh | Jain S.K.,Veterinary Science University of Madhya Pradesh | Sarkhel B.C.,Veterinary Science University of Madhya Pradesh
Animal Biotechnology | Year: 2012

RNAi is an evolutionary conserved, highly efficient, and cost effective technique of gene silencing. It holds considerable promise and success has been achieved both in vitro and in vivo experiments. However, it is not devoid of undesirable side effects as dsRNA can trigger the immune response and can also cause non-specific off-target gene silencing. In the present study, silencing of myostatin gene, a negative regulator of myogenesis, was evaluated in caprine fetal fibroblasts using three different shRNA constructs. Out of these three constructs, two constructs sh1 and sh2 showed, 72% and 50% reduction (p<0.05) of myostatin mRNA, respectively. Efficient suppression (42-86%) of MSTN gene (p<0.05) was achieved even by reducing the concentration of shRNA constructs. The induction of classical interferon stimulated gene (Oligoandenylate Synthetase-1, OAS-1) was studied to analyze the immune response against shRNAs. Notably, a reduction in the potency of shRNAs to induce interferon response was observed at lower concentration for OAS1 gene. The results obtained in the study would be helpful in the abrogation of the bystander effects of RNAi for long term stable expression of anti-MSTN expression constructs in the muscle. © 2012 Taylor and Francis Group, LLC.


Kumar D.,Veterinary Science University of Madhya Pradesh | Sarkhel B.C.,Veterinary Science University of Madhya Pradesh
Livestock Science | Year: 2016

The present study demonstrated a new approach for production of zona free cloned embryos in goat wherein, instead of hand guided bisection applied under Hand-made cloning technique, we have adopted micromanipulator based enucleation using innovative microtools viz., Bisection blade and Aspiration pipette. Using bisection blade, oocyte was bisected into two halves and one half having polar body-MII chromosome was instantly discarded by aspiration pipette, resulted into 100% enucleation efficiency. This process did not require Hoechst staining for confirmation of enucleation. The electrofusion process was carried out using indigenously prepared electrodes for individual electrofusion of demicytoplasts-somatic cell triplet, which revealed significantly higher (P≤0.01) fusion rate (92.78±0.5%) as compared to conventional technique (64.61±0.93%) for zona intact embryos. The developmental rates of zona-free embryos were observed as significantly higher (P≤0.01) 2-4 cells (79.13±0.82 vs 29.42±0.55), 8-16 cells (66.68±01.02 vs 23.83±0.9), morula (31.93±1.12 vs 17.93±1.07), blastocysts (24.0±1.56 vs 7.79±0.98) and mean blastomere counts (210.3±2.3 vs 154.0±1.60) as compared to zona-intact embryos. © 2016 Elsevier B.V.


PubMed | Veterinary Science University of Madhya Pradesh
Type: Journal Article | Journal: Journal of parasitic diseases : official organ of the Indian Society for Parasitology | Year: 2016

The coproculture study on Strongyle infection of goats was carried out in small holder farmers kept under semi-intensive management system in Balaghat, Narsinghpur and Chhindwara district, Madhya Pradesh, during the period from July 2011 to February 2012. Copro-culture of the samples positive for Strongyle infection revealed Haemonchus sp., Trichostrongylus sp., Oesophagostomum sp., Strongyloides sp., and Bunostomum sp. in a decreasing order in goats. The larvae of Haemonchus sp. (61.63%) and Strongyloides sp. (7.50%) were highest in Balaghat, Trichostrongylus (18.13%) in Narsinghpur, while Oesophagostomum sp. (10.50%) and Bunostomum sp. (5.75%) were in Chhindwara district. The finding of this study indicates that, even though subclinical in nature, Strongyle infection are one of the major problems that could hamper health and productivity and there is need for design a programme to minimize and control Strongyle infection in goats in the study area.

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