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Ben Salem R.,Veterinary Research Institute of Tunisia | Ben Salem R.,University of Carthage | Abbassi M.S.,Veterinary Research Institute of Tunisia | Garcia V.,University of Oviedo | And 3 more authors.
Zoonoses and Public Health | Year: 2016

Salmonella enterica subsp. enterica serovar Eppendorf, with antigenic formula 1,4,12,[27]:d:1,5, is an infrequent serovar. However, 14% (20 of 142) of the isolates recovered during June-July 2012 in chicken farms in Tunisia belonged to S. Eppendorf. These isolates were analysed for resistance and virulence profiles. None of them were susceptible to all antimicrobials tested, while 70%, 60%, 50%, 50%, 20% and 5% were resistant to sulphonamides (sul1, sul2 and sul3), streptomycin (aadA1-like), trimethoprim (dfrA1-like), nalidixic acid (GyrA Asp87→Asn and not identified), gentamicin (not identified) and ampicillin (blaTEM-1-like). About 30% of the isolates showed decreased susceptibility to ciprofloxacin and carried the qnrB gene 65% of the isolates were multidrug resistant and contained class 1 integrons with sul1 or sul3 in the 3' conserved segment. The orgA, ssaQ, mgtC, siiD and sopB virulence genes located on SPI1 to SPI5 and the fimbrial bcfC gene were present in all isolates; the sopE1 and sodC1 carried by prophages were variably detected; however, the prophage gipA gene and the spvC gene of serovar-specific virulence plasmids were absent. Altogether, ten resistance and three virulence profiles were identified. Typing of the isolates with XbaI- and BlnI-PFGE supports a close relationship, although they appear to be evolving under selective pressure probably caused by antimicrobial use in chicken husbandry. As far as we know, this is the first study investigating the molecular bases of antimicrobial drug resistance, the virulence gene content and the PFGE profiles of S. Eppendorf. The epidemiological surveillance of this serovar would be necessary to evaluate its possible impact on human health, particularly in Tunisia and other African countries where it was already reported. © 2016 Blackwell Verlag GmbH. Source

Ben Kahla I.,Veterinary Research Institute of Tunisia | Ben Kahla I.,Microbiology and Immunology Laboratory | Boschiroli M.L.,Bacterial Zoonoses Unit Animal Health Laboratory French Agency for Food | Souissi F.,Veterinary Research Institute of Tunisia | And 4 more authors.
African Health Sciences | Year: 2011

Background: Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk. Methods: Three hundred and six milk samples collected from 102 infected cows in different Tunisian regions were analysed. M. bovis isolates were further characterized by spoligotyping and variable number tandem repeat typing. Results: A total of five (4.9%) M. bovis strains exhibiting three different genotypes were isolated. Conclusion: This study demonstrates that consumers of raw milk or derivatives in Tunisia are at high risk of zoonotic infection with M. bovis. Source

Haddad-Boubaker S.,Veterinary Research Institute of Tunisia | Boughdir W.,National Agronomic Institute of Tunisia | Sghaier S.,Veterinary Research Institute of Tunisia | Souissi J.B.,National Agronomic Institute of Tunisia | And 5 more authors.
Fish Pathology | Year: 2014

In this study, we report outbreak of viral nervous necrosis (VNN) in wild Epinephelus species, which are of an endangered fish group, in different Tunisian coastal areas in 2012. Seven fish of E. marginatus and E. costae caught at dead or moribund condition were investigated. Betanodavirus was detected in the brain and retinal tissues of all fish by RT-PCR and at high infective titers (106.0-8.8 TCID50/g) in five of seven fish. Sequence and phylogenic analyses of the viral genes revealed that the viruses belonged to RGNNV genotype and were closely related to some previously reported Mediterranean betanodavirus strains, suggesting virus exchanges among different fish populations in the Mediterranean Sea. Source

Haddad-Boubaker S.,Veterinary Research Institute of Tunisia | Fakhfakh E.,Veterinary Research Institute of Tunisia | Megdich A.,Veterinary Research Institute of Tunisia | Ben Chehida N.,Veterinary Research Institute of Tunisia
Fish Pathology | Year: 2013

Lymphocystis disease virus (LCDV) causes a self-limiting disease in fish. However, recently, LCD outbreaks have increased, causing significant mortalities. In this study, we report the occurrence of two disease outbreaks in reared gilt-head sea bream Sparus aurata in the Tunisian coast. Presence of LCDV in diseased fish was confirmed by virus isolation using BF-2 cells and PCR amplification targeting the polymerase gene. Amino acid sequence analysis of the major capsid protein suggested that the LCDV strain was identical to nine strains previously isolated from the Mediterranean, Red Sea and South Atlantic coasts of Europe. These strains might have been disseminated through recent international trade. Source

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