Sarajevo, Bosnia and Herzegovina
Sarajevo, Bosnia and Herzegovina

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Bilandzic N.,Croatian Veterinary Institute | Varenina I.,Croatian Veterinary Institute | Kolanovic B.S.,Croatian Veterinary Institute | Luburic .B.,Croatian Veterinary Institute | And 4 more authors.
Mljekarstvo | Year: 2016

A total of 548 raw milk samples were collected in the western, central and eastern regions of Croatia during February and March 2015. Aflatoxin M1 (AFM1) concentrations were quantified by the enzyme immunoassay method. The method limits of detection (LOD) and quantification (LOQ) were 22.2 and 34.2 ng/kg, respectively. The mean AFM1 levels measured in the three regions were (ng/kg) as follows: western 3.69, central 3.11 and eastern 4.14. In total, the 548 samples analysed concentrations were below the LOD value and accordingly below the European Union maximum residue level (EU MRL) of 50 ng/kg. The results suggest an absence of use of contaminated with afla-toxin B1 supplementary feedstuff for lactating cows in winter 2015. Such results might be related to the improved storage conditions for feed as well as to the enhanced and more stringent feed control system for mycotoxins in Croatia. © 2016, Hrvatska Mljekarska Udruga. All rights reserved.


Bilandzic N.,Croatian Veterinary Institute | Bozic T.,Croatian Veterinary Institute | Dokic M.,Croatian Veterinary Institute | Sedak M.,Croatian Veterinary Institute | And 4 more authors.
Food Control | Year: 2014

Aflatoxin M1 contamination was examined in raw milk (3716) and UHT milk (706) samples collected from farms of eastern Croatia and markets of central Croatia from February to July 2013. A maximal mean AFM1 of 1135.0ng/L was measured in raw milk in March. The AFM1 levels exceeded the European Union maximum residue permitted amount (EU MRL) in 45.9% raw and 36.2% UHT milk samples in February. In total, AFM1 levels exceeded the EU MRL values in 27.8% of raw and 9.64% of UHT milk samples. A slight decrease in the number of samples exceeding the EU MRLs was recorded in the period March to June. The results showed significant statistical differences between the mean AFM1 concentrations of raw and UHT milk samples collected during February, March, May and June (P<0.05, all). Also, statistical differences in AFM1 concentrations were found between months for raw and UHT milk (P<0.001, both). In conclusion, the frequency of control of feed and milk samples should increase and should strive to educate breeders and those involved in milk production about the harmful effect of aflatoxins to animal feed. © 2013 Elsevier Ltd.


Bilandzic N.,Croatian Veterinary Institute | Tankovic S.,Veterinary Office of Bosnia and Herzegovina | Varenina I.,Croatian Veterinary Institute | Kolanovic B.S.,Croatian Veterinary Institute | Smajlovic M.,University of Sarajevo
Bulletin of Environmental Contamination and Toxicology | Year: 2012

Depletion of chloramphenicol (CAP) in muscle of rainbow trout was evaluated following 4 days of oral administration with two dosages (42 and 84 mg/kg/day). Sampling was conducted during treatment and for 35 days following the end of treatment. Analysis was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Concentrations observed during treatment were more than 300 μg/kg. A significant elimination occurred within 9 days after the cessation of treatment in both groups. Higher CAP levels were measured in the group treated with higher dose. CAP was not detectable after 13 and 15 after the end of treatment in both groups. © Springer Science+Business Media, LLC 2012.


Bilandzic N.,Croatian Veterinary Institute | Varenina I.,Croatian Veterinary Institute | Tankovic S.,Veterinary Office of Bosnia and Herzegovina | Kolanovic B.,Croatian Veterinary Institute
Arhiv za Higijenu Rada i Toksikologiju | Year: 2011

Chloramphenicol muscle residue levels in rainbow trout were determined after oral administration of 84 μg kg -1d -1 of chloramphenicol for four days. Samples were taken one day before treatment and for 43 days after the treatment was over. Chloramphenicol was analysed using an in-house enzyme linked immunoassay (ELISA) validated against the criteria of the Commission Decision 2002/657/EC. Validation parameters confirmed that the method was appropriate for the detection of chloramphenicol at levels below the minimum required performance limit (MRPL) of 0.3 μg kg -1. The highest chloramphenicol levels were observed on the first day after the treatment had ended (144.3 μg kg -1). Elimination was significant over the first seven days; significant differences were detected between days 1 and 3 (p<0.001), 3 and 5 (p<0.001), and 5 and 7 (p<0.05). Chloramphenicol levels dropped below MRPL to 0.17 μg kg -1 on day 9 after the end of treatment. From day 11 to 43, chloramphenicol residues were detectable in a range from 0.091 μg kg -1 (highest) to 0.011 μg kg -1 (lowest). Our results indicate that trout muscle tissue could be compliant with health requirements for consumption 10 days after withdrawal from chloramphenicol treatment.


Bilandzic N.,Croatian Veterinary Institute | Tankovic S.,Veterinary Office of Bosnia and Herzegovina | Jelusic V.,Veterinary Office of Bosnia and Herzegovina | Varenina I.,Croatian Veterinary Institute | And 3 more authors.
Food Control | Year: 2016

Aflatoxin M1 (AFM1) concentrations were determined in raw and UHT cow milk samples collected in different regions of Bosnia and Herzegovina and Croatia during the autumn months of 2014. The mean AFM1 levels in the raw milk samples were (ng/kg): 6.22 in Bosnia and Herzegovina, 5.65 in Croatia. In all except one milk sample, AMF1 levels were below the LOQ value of 34.2 ng/kg (ELISA method). In four milk samples, AFM1 concentrations exceeded the EU MRL of 50 ng/kg. Samples were subjected to LC-MS/MS analysis which confirmed elevated values determined by ELISA. Elevated levels were in the range 56.6-132.6 ng/kg. Two positive milk samples from Bosnia and Herzegovina originated from Una Sana Canton, two from Croatia from eastern Croatia. The highest AFM1 levels of 132.6 ng/kg was measured in milk from eastern Croatia. In 214 samples of processed UHT milk from Bosnia and Herzegovina and Croatia, AFM1 ranged from 2.29 ng/kg to 21.4 ng/kg, all below the LOQ value. AFM1 exceeded the EU MRL value in only 0.62% of milk samples, indicating the sporadic use of contaminated feedstuff at farms in both countries. © 2016 Elsevier Ltd.


Bilandzic N.,Croatian Veterinary Institute | Varenina I.,Croatian Veterinary Institute | Kolanovic B.S.,Croatian Veterinary Institute | Bozic T.,Croatian Veterinary Institute | And 5 more authors.
Food Control | Year: 2015

A total of 3543 raw cow milk samples were collected in three regions of Croatia: western, eastern and other regions during four seasons. Samples were measured for aflatoxin M1 (AFM1) concentrations using the enzyme immunoassay method. Elevated levels (>50ng/kg) of AFM1 were analysed by validated liquid chromatography with triple quadruple mass spectrometry (LC-MS/MS). The limits of detection (LOD) and quantification (LOQ) of the LC-MS/MS method were 7.3 and 28ng/kg, respectively. The mean AFM1 levels measured in the three regions over four seasons were in the ranges (ng/kg): eastern Croatia 7.25-26.6; western Croatia 5.91-9.26; other regions of Croatia 7.17-13.6. The highest incidence of samples exceeding the EU MRL (50ng/kg) of 9.32% was measured in autumn (October-December) in the eastern region. Only eight samples were found to exceed the EU MRL in winter. The highest AFM1 levels were measured in December (764.4ng/kg) and January (383.3ng/kg). Elevated AFM1 levels were found in summer in only four samples from the western and other regions, and two samples in the eastern region. This can be attributed to localized and random usage of contaminated feed for dairy cows in those regions. The much lower incidence of elevated AFM1 in comparison to a previous study showed that the outbreak of the crisis due to elevated AFM1 levels in 2013 resulted in a more careful approach to the control of supplementary feedstuff for lactating cows. © 2015 Elsevier Ltd.

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