Veterinary Institute Krizevci

Križevci, Croatia

Veterinary Institute Krizevci

Križevci, Croatia
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Bilandzic N.,Croatian Veterinary Institute | Sedak M.,Croatian Veterinary Institute | Dokic M.,Croatian Veterinary Institute | Varenina I.,Croatian Veterinary Institute | And 3 more authors.
Slovenian Veterinary Research | Year: 2014

The concentrations of macro- and microelements in the milk of Croatian Coldblood mares were determined throughout the lactation phases by inductively coupled plasma-optical emission spectrometry. Element contents during days 10-180 of lactation were in the ranges (mg/kg): Ca 585-772, K 547-687, Na 131-165, Mg 56.8-71.0, Cu 0.085-0.14, Fe 0.013-0.41, Zn 1.86-2.15, Se 0.003-0.029. Variation trends for elements were found throughout the lactation stages. However, significantly higher Fe concentrations were found on days 10 and 40 of lactation than on day 60 (p < 0.05, both). There were no statistically significant differences between concentrations of other elements during lactation. Moderate correlations were found between the elements: Ca: K, Mg, Se; Mg: K, Cu; Se: Cu, Fe. The element concentrations in horse milk, with the exception of Ca and Cu, fell within the ranges previously reported for different breeds of dairy horses. Cu and Fe contents were lower, while the Zn content was similar to those obtained in different dairy horse breeds. Concentrations of elements obtained in horse milk were lower in comparison to cow and goat milk. Generally, levels of Ca and Mg were higher, Na and K were similar, but Cu, Fe and Zn were lower than those in human milk.


Bilandzic N.,Croatian Veterinary Institute | Sedak M.,Croatian Veterinary Institute | Dokic M.,Croatian Veterinary Institute | Kolanovic B.S.,Croatian Veterinary Institute | And 3 more authors.
Mljekarstvo | Year: 2014

The concentrations of essential elements in the milk of Croatian Coldblood mares and Littoral-Dinaric donkey were measured. The mean element contents in horse milk were (mg/kg): Ca 687.1, Mg 72.1, K 637.7, Na 143.5, Cu 0.126, Fe 0.235, Se 0.01, Zn 2.06, Mn<0.01. In donkey milk, the mean element contents were (mg/kg): Ca 815.4, Mg 80.8, K 887.7, Na 217.6, Cu 0.027, Fe 0.196, Zn 2.36, Mn 0.016. In horse milk, moderate correlations were found between the elements: Ca-K, Mg-Se, Mg-K, Mg-Cu, Se-Cu, Se-Fe, Mn-K. Strong correlations were found among elements in donkey milk: Ca-Na, Ca-K, Ca-Cu, K-Na, K-Cu, Cu-Mn. Significant differences between Na, K and Cu concentrations were obtained between the two species. Other elements concentrations were similar in both species.


Bilandzic N.,Croatian Veterinary Institute | Gacic M.,University of Zagreb | Dokic M.,Croatian Veterinary Institute | Sedak M.,Croatian Veterinary Institute | And 3 more authors.
Journal of Food Composition and Analysis | Year: 2014

Different honey types were collected in Croatia during 2010 and 2011: 7 multifloral orchard honeys, 7 multifloral meadow honeys, 19 black locust, 9 chestnut, 11 lime and 6 sage honeys. Elements were measured using graphite (As, Cu, Cd, Pb, Se) and flame atomic absorption spectrometer (Ca, Fe, K, Mg, Na, Zn) and by mercury analyser (Hg). Significant differences in Ca, Fe, K, Mg, Zn, As and Hg levels were observed between honey types. In chestnut honey were determined (K, Ca, Mg: mgkg-1; Hg, Ad, Cd: μgkg-1): the highest concentrations of K 2824.4, Ca 486.7, Mg 59.1 and Hg 2.52; the lowest of As 24.1 and Cd 2.52. Lime honey has been shown the highest content of Cu (20.6mgkg-1), Zn (6.78mgkg-1), Cd (2.14μgkg-1) and Pb (810.3μgkg-1). The lowest levels of following elements were determined in black locust honey (Fe, K, Mg: mgkg-1; Hg: μgkg-1): Fe 2.77, K 304.7, Mg 8.02 and Hg 0.82. Sage honey had the lowest Ca and Na content (173.9 and 31.8mgkg-1). Among the multifloral honeys, the following was determined (Fe, Na, Cu: mgkg-1; As, Pb: μgkg-1): orchard honey - highest of Fe 5.17 and As 276.1, lowest Pb 301; meadow honey - highest Na 36.1, lowest Cu 4.38. The average Ca, Cu and Pb levels found in multifloral honey types were much higher than those reported in other European countries. Calcium levels in lime, chestnut and black locust were higher than those from other countries. © 2014 Elsevier Inc.


Bilandzic N.,Croatian Veterinary Institute | Dokic M.,Croatian Veterinary Institute | Sedak M.,Croatian Veterinary Institute | Kolanovic B.S.,Croatian Veterinary Institute | And 3 more authors.
Food Chemistry | Year: 2011

The concentrations of As, Cd, Cu, Hg and Pb were determined in 54 multi-floral honey samples collected from five regions of Croatia during 2009 and 2010. Element contents decreased in the following order: Cu > Pb > As > Hg > Cd. Significant differences in lead and copper levels were observed between regions. Mean levels of elements (μg kg-1) in all honey samples measured were: 19.7 for As, 1.51 for Cd, 1074 for Cu, 2.72 for Hg and 65.2 for Pb. Copper and lead were the most abundant elements in the Centre region, with range and mean contents of 108-41,271 and 3232 μg kg -1 and 22.0-440 and 131 μg kg-1, respectively. The highest element contents were: As 23.8 μg kg-1 in the South region, Cd 2.11 μg kg-1 in the Southwest region and Hg 2.63 μg kg-1 in the Northeast region. The finding that lead contents in Croatian honey were higher than most reported lead levels in honey from other European countries is of particular concern. These indicate that attention must be focused on setting positions for honey production hives in areas distant from highways and railways. © 2011 Elsevier Ltd. All rights reserved.


Bilandzic N.,Croatian Veterinary Institute | Sedak M.,Croatian Veterinary Institute | Dokic M.,Croatian Veterinary Institute | Kolanovic B.S.,Croatian Veterinary Institute | And 3 more authors.
Mljekarstvo | Year: 2013

Microelements concentrations of Al, Co, Cr, Li, Mo, Ni, Sb and Sr were determined in the milk of Croatian Coldblood mares by inductively coupled plasma-optical emission spectrometry. The total mean element contents in mares milk were (μig/kg): Al<10, Co<10, Cr 26.7, Li 4.35, Mo 11.0, Ni<10, Sb 70.1 and Sr 467. Variation of Li, Sb and Sr concentrations were found throughout the lactation stages (days 10-180). Correlations were found among elements in mares milk. In general, very limited data are available on the microelements content of Al, Cr, Co, Li, Mo, Ni, Sb and Sr in mares milk. To our knowledge this is the first report of the content of these microelements in milk of mares in Croatia.

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