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Oliveira do Hospital, Portugal

Lopes A.P.,University of Tras os Montes e Alto Douro | Lopes A.P.,Animal and Veterinary Research Center | Sousa S.,University of Porto | Dubey J.,Beltsville Agricultural Research Center | And 8 more authors.
Parasites and Vectors | Year: 2013

Background: Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal. Findings. Antibodies to L. infantum were detected by the direct agglutination test (DAT); seven (4.0%) horses were seropositive with DAT titres of 200 (n = 5), 800 (n = 1) and ≥ 1600 (n = 1). Antibodies to T. gondii were assayed by the modified agglutination test (MAT); 23 (13.3%) horses were seropositive with MAT titres of 20 (n = 13), 40 (n = 5), 80 (n = 3) and ≥ 160 (n = 2). No statistical differences were found among equine categories of gender (female, male and gelding), age (1.5-6, 7-12 and 13-30 years), type of housing (indoors and mixed/outdoors), ability (recreation, farming and sports) and clinical status (apparently healthy and sick) for both agents. Conclusions: Horses are exposed to and may be infected with L. infantum and T. gondii in the north of Portugal. © 2013 Lopes et al.; licensee BioMed Central Ltd.

Jattennavar P.S.,Veterinary Hospital | Kalmath G.P.,Veterinary College
Indian Journal of Animal Research | Year: 2012

One and half year old male Pomeranian dog was presented to the veterinary hospital, Gadag, with the complaint of tenesmus, uneasiness, passing scanty urine, severe straining during urination and passing of blood mixed urine at the end of the urination for the past 5 days and anuria for the past 24 hours. Upon clinical examination, animal was dull, showing symptoms of abdominal pain and dehydration. Temperature was sub normal and respiratory and pulse rate were elevated. Ventral abdominal palpation along the course of penis revealed, a hard granular mass just posterior to the os penis. When the attempts to dislodge calculus in to the urinary bladder failed, the animal was operated under atropine suplhate and thiopentone sodium to remove a round 6 mm calculus. Post surgical treatment with antibiotics and daily dressing of the surgical for a week ensured recovery of animal and re-establishment of urine flow.

Al-Mayah A.A.S.,University of Basrah | Tabeekh M.A.S.A.,Veterinary Hospital
International Journal of Poultry Science | Year: 2010

Forty five Hubbard broiler chicks and Forty five local chicks of one day old were reared on litter floor for 35 days. The chicks were divided into six groups A, B and C for broiler chicks, D, E and F for local chicks. The chicks of group A and D were vaccinated with an intermediate vaccine (Bursine®-2) whereas, the chicks of group B and E were vaccinated with an intermediate-plus type of vaccine (Bursine® Plus). Vaccination has been performed at 14t h day. The chicks of group C and F were acted as control. Five chicks were sacrificed by decapitation from the experimental groups at 21s t, 28t h and 35t h day and the bursae were removed, bursa weight (gram) and body weight (gram) were recorded for each individual bird. Significant decrease of bursa weight (p<0.05) was found in group B compared with that of group C at 2 1 s t day. The bursa weights in group D and E of local chicks were significantly differed at (p<0.05) from that of control group (F). The body weights of group D and E of local chicks were significantly decreased from those of group F at (p<0.05). The present study revealed that the more pathogenic intermediate vaccine (intermediate-plus) caused severe bursa of Fabricius injury in vaccinated local chicks. This could be explained by the lower degree of attenuation of this kind of vaccine and may be also related to the lack of maternally-derived antibody in these chicks. © Asian Network for Scientific Information, 2010.

Gao G.,University of Massachusetts Medical School | Bish L.T.,University of Pennsylvania | Sleeper M.M.,Veterinary Hospital | Mu X.,University of Massachusetts Medical School | And 6 more authors.
Human Gene Therapy | Year: 2011

Heart disease is the leading cause of morbidity and mortality, and cardiac gene transfer has potential as a novel therapeutic approach. We previously demonstrated safe and efficient gene transfer to the canine heart using a percutaneous transendocardial injection procedure to deliver self-complementary (sc) adeno-associated virus 6 (AAV6) vector. In the present study, we proceed with our vertical translation study to evaluate cardiac gene transfer in nonhuman primates (NHPs). We screened approximately 30 adult male rhesus macaques for the presence of neutralizing antibodies against AAV6, AAV8, and AAV9, and then selected seven monkeys whose antibody titers against these three serotypes were lower than 1/5. The animals were then randomized to receive either scAAV6 (n=3), scAAV8 (n=1), or scAAV9 (n=3) vector expressing the enhanced green fluorescent protein (EGFP) reporter gene at a dose of 5.4×10 12 genome copies/kg, which was administered according to a modified version of our previously developed transendocardial injection procedure. One animal treated with scAAV6 died secondary to esophageal intubation. The remaining animals were euthanized 7 days after gene transfer, at which time tissue was collected for analysis of EGFP expression, histopathology, and biodistribution of the vector genome. We found that (i) transendocardial delivery of AAV is safe in the NHP, (ii) AAV6 and AAV8 provide efficient cardiac gene transfer at similar levels and are superior to AAV9, and (iii) AAV6 is more cardiac-specific than AAV8 and AAV9. The results of this NHP study may help guide the development AAV vectors for the treatment of cardiovascular disease in humans. © 2011 Mary Ann Liebert, Inc.

Ochube G.E.,Veterinary Hospital | Kaltungo B.Y.,Veterinary Teaching Hospital | Abubakar U.B.,Ahmadu Bello University
Journal of Animal and Veterinary Advances | Year: 2014

Congenital anomaly by autosomal recessive genes has been implicated as the main etiology of contracted flexor tendons in musculoskeletal deformities of new borne calves. Surgical intervention of this case revealed that ankylosis was caused by wrong origination and insertion of malformed ulnaris lateralis muscle. A stay in the form of splints was placed throughout the limb. Plasters of Paris were then applied to the whole length of the limb to enhance stability. The surgical site was fenestrated to allow aeration and dressing, the overall result of the procedure was encouraging. After 7 weeks, a second surgeiy was performed to correct the bilateral contracted flexor tendon of the carpal joint. This was achieved by deep digital flexor tendon tenotomy of the carpal joint and placing a fiber glass cast on both joints. © Medwell Journals, 2014.

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