Badiei A.,Islamic Azad University at Karaj |
Moosakhani F.,Islamic Azad University at Karaj |
Hamidi A.,Mabna Veterinary Diagnostic Laboratory |
Sami M.,Ferdowsi University of Mashhad
Journal of Dairy Science | Year: 2013
The objective of this study was to evaluate the effect of Protexin (Probiotics International Ltd., South Petherton, UK) in the prevention of ileocecal infection by Mycobacterium avium ssp. paratuberculosis (MAP) in dairy calves in the field situation. Forty Holstein bull calves whose dams were paratuberculosis negative (confirmed by serum ELISA test and fecal nested PCR) were randomly selected in 2 groups. All calves were fed raw milk collected from the bulk tank in a paratuberculosis-infected dairy farm, which was confirmed by PCR. The treatment group (20 calves) was given 2. g of Protexin from birth until weaning (90. d). The control group (20 calves) did not consume Protexin. The calves were culled at 12. mo of age and the ileocecal lymph nodes were sampled. The lymph nodes were tested by nested PCR to evaluate MAP infection. In the treatment group, 2 out of 20 calf (10%) ileoceca were infected by MAP, whereas in the control group, 8 out of 20 calf (40%) ileoceca were infected by MAP. A significant difference existed between ileocecal infection by MAP in treatment and control groups. Thus, Protexin showed a significant effect in decreasing the ileocecal infection by MAP. © 2013 American Dairy Science Association.