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Sahin K.,Firat University | Orhan C.,Firat University | Tuzcu M.,Firat University | Sahin N.,Firat University | And 6 more authors.
Nutrition and Cancer | Year: 2014

Hepatocarcinogenesis is one of the most prevalent and lethal cancers. We studied the mechanisms underlying the inhibition of diethylnitrosamine (DEN)-induced hepatocarcinogenesis by lycopene in rats. Hepatocarcinogenesis was induced by an intraperitoneal injection of DEN followed by promotion with phenobarbital for 24 successive wk. The rats were given lycopene (20 mg/kg body weight) 3 times a week orally for 4 wk prior to initiation, and the treatment was continued for 24 consecutive wk. Lycopene reduced incidence, number, size, and volume of hepatic nodules. Serum alanine transaminase, aspartate aminotransferase, total bilirubin, and malondialdehyde (MDA) considerably increased and hepatic antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase) and glutathione decreased in DEN-treated rats when compared with the control group. Lycopene significantly reversed these biochemical changes and increased the expression of NF-E-2-related factor-2)/heme oxygenase-1, and it decreased NF-B/cyclooxygenase-2, inhibiting the inflammatory cascade and activating antioxidant signaling (P < 0.05). Lycopene also decreased DEN-induced increases in phosphorylated mammalian target of rapamycin (p-mTOR), phosphorylated p70 ribosomal protein S6 kinase 1, phosphorylated 4E-binding protein 1, and protein kinase B (P < 0.05). Lycopene is an active chemopreventive agent that offers protection against DEN-induced hepatocarcinogenesis by inhibiting NF-B and mTOR pathways. © 2014 Copyright Taylor & Francis Group, LLC.

Erol N.,Adnan Menderes University | Gurcay M.,Institute of Veterinary Control | Krdar S.,Adnan Menderes University | Ertugrul B.,Adnan Menderes University | And 3 more authors.
Israel Journal of Veterinary Medicine | Year: 2016

West Nile Virus (WNV) infection, transmitted mainly through mosquito bites, is generally asymptomatic but may also result in mild fever, meningitis, encephalitis and death in various animals and humans. The presence of the WNV in Turkey has been reported previously. However, comparative epizootiological data on the recent prevalence of the WNV infection in various animal species and humans in western Turkey are limited. The objective of this study was to investigate the seroprevalence of WNV infection in animals and humans in western Turkey. Four hundred and forty serum samples were collected from 40 cats, 60 cattle, 90 humans and 50 sheep, goats, camels, horses, and broiler breeder chickens between the years 2009 and 2012. The serum samples were tested for antibodies against the envelope protein (E) of the virus using a commercial competitive Enzyme-Linked Immunosorbent Assay (cELISA). The human samples were retested using commercial Indirect Fluorescence Antibody Test (IFAT). No antibodies were detected in cats, sheep, goats, and chickens. Seropositivity rates of 44% (22/50) in camels, 32% (16/50) in horses, 20% (12/60) in cattle and 41.1% (37/90) in humans were found. The results of the seropositivity in humans obtained using cELISA and IFAT were identical. These data suggest that the WNV infection may be present subclinically in horses, camels, cattle and humans in western Turkey and may pose a threat to animal and human health in the region and surrounding areas. © 2016, Israel Veterinary Medical Association. All rights reserved.

Sahin K.,Firat University | Orhan C.,Firat University | Tuzcu M.,Firat University | Muqbil I.,Aligarh Muslim University | And 6 more authors.
Biological Trace Element Research | Year: 2014

Curcumin, a polyphenol, has pharmacological effects including antioxidant, anti-inflammatory and anti-cancer features. In this study, we have performed comparative in vivo evaluations of CDF (curcumin difluorinated) and curcumin in cisplatin-induced nephrotoxicity in rats. Male Wistar rats were divided into four groups: (1) Control; (2) Cisplatin (7 mg/kg body wt, intraperitoneal as a single dose); (3) Cisplatin and CDF (50 mg/rat/day; for 12 days); (4) Cisplatin and curcumin (50 mg/rat/day), for 12 days). Cisplatin treated rats exhibited kidney injury manifested by increased serum N-urea and creatinine (P < 0.001). Kidney from cisplatin treated rats also exhibited significant increase in malondialdehyde (MDA) and 8-isoprostane levels (P < 0.001). Treatment with CDF and curcumin prevented the rise in serum N-urea, creatinine, MDA and 8-isoprostane as compared to experimental control group in kidney (P < 0.05). Compared to curcumin, CDF had greater potential in suppressing cisplatin-induced pro-inflammatory factors NF-κB and COX-2 as well as downstream markers Nrf2 and HO-1 (P < 0.05) in kidney. The analysis on anion transport markers (OAT1 and OAT3) showed a similar trend (CDF > curcumin). CDF could reduce the expression of multi-drug resistance markers OCT1, OCT2, MRP2 and MRP4 to a much greater extent than curcumin (P < 0.05). We also demonstrate that CDF influenced the expression of p-mTOR, p-p70S6K1, p-4E-BP1 and p-Akt. These data suggest that CDF can potentially be used to reduce the chemotherapy induced nephrotoxicity thereby enhancing the therapeutic window of cisplatin. The results also proved that compared to curcumin, CDF has superior protective effect in nephrotoxicity. © 2014 Springer Science+Business Media New York.

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