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Kevenk T.O.,Veterinary Control Central Research Institute | Terzi Gulel G.,Ondokuz Mayis University
Journal of Food Safety | Year: 2016

The objectives of study were to assess presence of Listeria monocytogenes, perform serotyping and investigate antibiotic resistance in raw milk and dairy products. A total of 210 milk and dairy products including white (n=20) and kashar cheese (n=20), ice cream (n=20), butter (n=20), cokelek (n=10), kuymak (n=10) and farm cheese (n=10) were obtained from Samsun, Turkey. All samples were analyzed using an immunomagnetic separation-based culture technique and strains of L.monocytogenes were confirmed by presence of hlyA and iap genes by polymerase chain reaction (PCR). L.monocytogenes was identified in 5 of 100 (5%) milk samples, serotyped as 4b and 1/2b, and in 9 of 110 (8.2%) dairy products, serotyped as 1/2a, 1/2b and 1/2c. However, L.monocytogenes was not identified from butter, kashar and ice cream samples. The antibiotic susceptibility against ampicillin, amoxicillin/clavulanic acid, erythromycin, chloramphenicol, penicillin G, oxytetracycline, tetracycline and vancomycin was assessed by disc diffusion method. It was found that 15.3% of isolates were resistant to at least one drug and 36.5% were multidrug resistant. Among isolates, resistance to tetracycline was most commonly encountered (34.6%), followed by resistance to chloramphenicol (25%) and penicillin G (23%). In conclusion, our data also indicate that consuming raw and unpasteurised milk and dairy products could pose a risk of listeriosis in humans. Practical Applications: Although there are plenty of studies about the incidence of L.monocytogenes in raw milk and dairy products in Turkey, our study is the first one in Samsun (Middle Black Sea) Region about Listeria in milk and dairy products. Also in our study, we characterized some virulence genes and serotype distribution of L.monocytogenes by PCR. Finally, we performed antibiotic resistance tests of isolates to see possible public health threats because of using overabundant antibiotics. If we analyze all these work to see the potential risk assessments in this region, our study could be a leading study in near future. © 2016 Wiley Periodicals, Inc.


Balkaya I.,Ataturk University | Utuk A.E.,Cukurova University | Babur C.,Refik Saydam National Hygiene Center | Beyhan Y.E.,Yuzuncu Yil University | And 2 more authors.
Israel Journal of Veterinary Medicine | Year: 2015

The aim of this study was to examine for the presence of anti-Toxoplasma gondii and Neospora caninum antibodies in wild boars and to study the impact of infection between the sylvatic and domestic life cycles of these apicomplexan parasites. For this purpose, sera were collected from hunter-killed wild boars (Sus scrofa) during the winter period of 2011 from Erzurum province of Turkey. Collected sera were examined for antibodies against T. gondii and N. caninum by Sabin Feldman dye test (SFDT) and competitive-enzymelinked immunosorbent assay (c-ELISA), respectively. Out of 12 collected samples, 4 (33.3%) of sera were found to be seropositive at the dilution of 1:16 for T. gondii however no seropositivity in any of the samples was detected against N. caninum. To the best knowledge of the authors, this is the first serologic study to detect anti-T. gondii antibodies in wild boars in Turkey. © 2015, Israel Veterinary Medical Association All rights reserved.


Karatas Yeni D.,Veterinary Control Central Research Institute | Izgur M.,Ankara University
Ankara Universitesi Veteriner Fakultesi Dergisi | Year: 2015

Tularemia is a zoonotic infection caused by Francisella tularensis and has gained renewed importance since there has been a recent increase in the number of human cases in several countries across the world. In this study the existence of F. tularensis in rodents and in water was investigated by culture and PCR techniques. Also, F. tularensis specific antibodies were investigated by serological methods in sheep. At the end of the study, F. tularensis was isolated from one water sample by culture and PCR techniques, on the other hand, 27.6% seropositivity was detected in the blood samples of sheep. © 2015, Chartered Inst. of Building Services Engineers. All rights reserved.


Us M.F.,Gazi University | Us M.F.,Veterinary Control Central Research Institute | Alshana U.,Gazi University | Lubbad I.,Gazi University | And 2 more authors.
Electrophoresis | Year: 2013

Dispersive liquid-liquid microextraction based on solidification of floating organic drop (DLLME-SFO) was for the first time combined with field-amplified sample injection (FASI) in CE to determine four β2-agonists (cimbuterol, clenbuterol, mabuterol, and mapenterol) in bovine urine. Optimum BGE consisted of 20 mM borate buffer and 0.1 mM SDS. Using salting-out extraction, β2-agonists were extracted into ACN that was then used as the disperser solvent in DLLME-SFO. Optimum DLLME-SFO conditions were: 1.0 mL ACN, 50 μL 1-undecanol (extraction solvent), total extraction time 1.5 min, no salt addition. Back extraction into an aqueous solution (pH 2.0) facilitated direct injection of β2-agonists into CE. Compared to conventional CZE, DLLME-SFO-FASI-CE achieved sensitivity enhancement factors of 41-1046 resulting in LODs in the range of 1.80-37.0 μg L-1. Linear dynamic ranges of 0.15-10.0 mg L-1 for cimbuterol and 15-1000 μg L-1 for the other analytes were obtained with coefficients of determination (R2) ≥ 0.9901 and RSD% ≤5.5 (n = 5). Finally, the applicability of the proposed method was successfully confirmed by determination of the four β2-agonists in spiked bovine urine samples and accuracy higher than 96.0% was obtained. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Utuk A.E.,Veterinary Control Central Research Institute | Piskin F.C.,Veterinary Control Central Research Institute
The Scientific World Journal | Year: 2012

The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey. © 2012 Armagan Erdem Utuk and Fatma Cigdem Piskin.

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