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Balkaya I.,Atatürk University | Utuk A.E.,Cukurova University | Babur C.,Refik Saydam National Hygiene Center | Beyhan Y.E.,Yuzuncu Yil University | And 2 more authors.
Israel Journal of Veterinary Medicine | Year: 2015

The aim of this study was to examine for the presence of anti-Toxoplasma gondii and Neospora caninum antibodies in wild boars and to study the impact of infection between the sylvatic and domestic life cycles of these apicomplexan parasites. For this purpose, sera were collected from hunter-killed wild boars (Sus scrofa) during the winter period of 2011 from Erzurum province of Turkey. Collected sera were examined for antibodies against T. gondii and N. caninum by Sabin Feldman dye test (SFDT) and competitive-enzymelinked immunosorbent assay (c-ELISA), respectively. Out of 12 collected samples, 4 (33.3%) of sera were found to be seropositive at the dilution of 1:16 for T. gondii however no seropositivity in any of the samples was detected against N. caninum. To the best knowledge of the authors, this is the first serologic study to detect anti-T. gondii antibodies in wild boars in Turkey. © 2015, Israel Veterinary Medical Association All rights reserved.


Gunaydin E.,Veterinary Control Central Research Institute | Pekkaya S.,Veterinary Control Central Research Institute | Mustak H.K.,Diskapi | Dalkilic B.,University of Gaziantep
Ankara Universitesi Veteriner Fakultesi Dergisi | Year: 2014

This study was carried out on Kilis and Shamil goats to determine the different stages of Q fever-chronic and acute- in serum samples by indirect ELISA and in blood samples by touchdown-PCR, respectively. A total of 92 serum samples comprising 46 Kilis and 46 Shamil goats was examined and 7.60%, 4.34% and 10.8% seropositivity were determined for total serum samples, Kilis and Shamil goat serum samples, respectively. Of the examined 92 blood samples belonging to 46 Kilis and 46 Shamil goats, none of them yielded 687 bp PCR products by touchdown-PCR. The positive ELISA titers were thought to be the evidence of previous infection. The province of Kilis localized in South-East Anatolian Region of Turkey was decided to be a risky region for Q fever.


Karatas Yeni D.,Veterinary Control Central Research Institute | Izgur M.,Ankara University
Ankara Universitesi Veteriner Fakultesi Dergisi | Year: 2015

Tularemia is a zoonotic infection caused by Francisella tularensis and has gained renewed importance since there has been a recent increase in the number of human cases in several countries across the world. In this study the existence of F. tularensis in rodents and in water was investigated by culture and PCR techniques. Also, F. tularensis specific antibodies were investigated by serological methods in sheep. At the end of the study, F. tularensis was isolated from one water sample by culture and PCR techniques, on the other hand, 27.6% seropositivity was detected in the blood samples of sheep. © 2015, Chartered Inst. of Building Services Engineers. All rights reserved.


Kuzukiran O.,Veterinary Control Central Research Institute | Yurdakok-Dikmen B.,Ankara University | Totan F.E.,Ankara University | Celik C.,Ankara University | And 4 more authors.
International Journal of Environmental Research | Year: 2016

Many of the persistent organic pollutants (POPs) with endocrine disrupting properties are monitored regularly by risk assessors with limited resources, where analytical procedures are usually laborious, expensive, and not ecofriendly. Moreover, these analyses were frequently advanced aiming one class of pollutants, consequently inefficient to correspond the demand of monitoring a quickly rising number of pollutants in the environment. The objective of this study was to develop a single sample extraction procedure and multiple gas chromatography-mass spectrometry runs for the detection of various groups of semi volatile organics; Polychlorinated Biphenyls (28, 52, 101, 118, 138, 153, 180), Polybrominated Diphenyl Ethers (17, 47, 66, 100, 153, 183) and Organochlorine Pesticides (α-HCH, HCB, γ-HCH, Heptachlor, p,p-DDD, p,p-DDE, p,p-DDT) in sediment and water samples simultaneously. Extraction for water involved solid phase extraction using C18 and for sediment using homemade column with florisil, primary secondary amine and magnesium sulfate with ultrasonication step by acetone. This procedure was validated and applied to water samples from tap, river and lake; and sediment samples from river and lake. For both matrices and all analytes, high linearity, recovery (88-106%) with all relative standart deviation values <20% and limit of quantification levels below the tested limits were achieved. This reliable and cost effective procedure for monitoring selected multiple POP levels in water and sediment; do not require complicated device nor intensive manual efforts, which would also minimize the depletion of the organic solvents, could be used for routine detection of selected POPs. © 2016, University of Tehran. All rights reserved.


Us M.F.,Gazi University | Us M.F.,Veterinary Control Central Research Institute | Alshana U.,Gazi University | Lubbad I.,Gazi University | And 2 more authors.
Electrophoresis | Year: 2013

Dispersive liquid-liquid microextraction based on solidification of floating organic drop (DLLME-SFO) was for the first time combined with field-amplified sample injection (FASI) in CE to determine four β2-agonists (cimbuterol, clenbuterol, mabuterol, and mapenterol) in bovine urine. Optimum BGE consisted of 20 mM borate buffer and 0.1 mM SDS. Using salting-out extraction, β2-agonists were extracted into ACN that was then used as the disperser solvent in DLLME-SFO. Optimum DLLME-SFO conditions were: 1.0 mL ACN, 50 μL 1-undecanol (extraction solvent), total extraction time 1.5 min, no salt addition. Back extraction into an aqueous solution (pH 2.0) facilitated direct injection of β2-agonists into CE. Compared to conventional CZE, DLLME-SFO-FASI-CE achieved sensitivity enhancement factors of 41-1046 resulting in LODs in the range of 1.80-37.0 μg L-1. Linear dynamic ranges of 0.15-10.0 mg L-1 for cimbuterol and 15-1000 μg L-1 for the other analytes were obtained with coefficients of determination (R2) ≥ 0.9901 and RSD% ≤5.5 (n = 5). Finally, the applicability of the proposed method was successfully confirmed by determination of the four β2-agonists in spiked bovine urine samples and accuracy higher than 96.0% was obtained. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Kuzukiran O.,Veterinary Control Central Research Institute | Yurdakok-Dikmen B.,Ankara University | Filazi A.,Ankara University | Sevin S.,Ankara University | And 2 more authors.
Analytical Letters | Year: 2016

A new method is reported to determine polychlorinated biphenyls (PCBs) in marine sediments using ultrasound-assisted extraction and dispersive liquid–liquid microextraction with gas chromatography–mass spectrometry. To optimize the method, acetone, acetonitrile, and methanol were characterized for extraction from the sediments. Acetone provided the highest yield. A lower-density solvent system was utilized with isooctane and acetone, and the upper solvent was characterized by gas chromatography–mass spectrometry. The optimized procedure was validated. Sediments were fortified with PCBs 28, 52, 101, 118, 138, 153, and 180 at six concentrations from 0.25 to 8.0 ng g−1 and used to prepare matrix-matched calibration curves. Samples were analyzed using this optimized procedure. The linearity was satisfactory in all cases, with correlation coefficients from 0.9989 to 0.9995. The limits of detection and quantification were from 0.021 to 0.057 ng g−1 and 0.069 to 0.190 ng g−1, respectively. The recovery values at three fortified concentrations were 90.07–100.4% and the relative standard deviations were less than 7.6%. The reported extraction method uses a low-density, low-toxicity solvent; a sample syringe as the extraction device; and does not require additional purification after extraction from sediment. The protocol is sensitive, convenient, and ecofriendly and was used to determine PCBs in marines with satisfactory results. © 2016, Copyright © Taylor & Francis Group, LLC.


Kuzukiran O.,Veterinary Control Central Research Institute | Filazi A.,Ankara University
Food Analytical Methods | Year: 2015

Polychlorinated biphenyl (PCB) residues in food are an important food safety concern. Simple and sensitive analytical methods are needed to monitor PCB residues and ensure that food is safe for consumption. The aim of this study was to adapt a selective, sensitive, quick, and easy sample treatment for purification of animal fat matrices and to measure the level of PCB28, PCB52, PCB101, PCB118, PCB138, PCB153, and PCB180 in samples of meat products (salami, soudjouk, and sausage) produced in Turkey. The extraction and purification of meat products were performed via the modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and PCB levels determined by gas chromatography–mass spectrometry. The linearity was satisfactory for all compounds studied, with correlation coefficients ≥0.99. The limits of determination and the limits of quantification were in the range of 0.144–0.382 and 0.479–1.274 ng g−1, respectively. Recovery at 3 different spiking concentrations was 95.7–101 % and the relative standard deviations were <3.5 %. This validated method was observed to be more economic and eco-friendly, as it uses a smaller volume of extraction solvents that are also less toxic. The validated method was successfully applied to the analysis of selected PCBs in meat products with satisfactory results. The method’s results indicated the presence of PCBs in some of the meat product samples, although the levels were below the maximum residue limit for food products of animal origin in Turkey (40 ng g−1 of fat), which is in accordance with EU and Turkish levels. © 2015 Springer Science+Business Media New York


Kevenk T.O.,Veterinary Control Central Research Institute | Terzi Gulel G.,Ondokuz Mayis University
Journal of Food Safety | Year: 2016

The objectives of study were to assess presence of Listeria monocytogenes, perform serotyping and investigate antibiotic resistance in raw milk and dairy products. A total of 210 milk and dairy products including white (n=20) and kashar cheese (n=20), ice cream (n=20), butter (n=20), cokelek (n=10), kuymak (n=10) and farm cheese (n=10) were obtained from Samsun, Turkey. All samples were analyzed using an immunomagnetic separation-based culture technique and strains of L.monocytogenes were confirmed by presence of hlyA and iap genes by polymerase chain reaction (PCR). L.monocytogenes was identified in 5 of 100 (5%) milk samples, serotyped as 4b and 1/2b, and in 9 of 110 (8.2%) dairy products, serotyped as 1/2a, 1/2b and 1/2c. However, L.monocytogenes was not identified from butter, kashar and ice cream samples. The antibiotic susceptibility against ampicillin, amoxicillin/clavulanic acid, erythromycin, chloramphenicol, penicillin G, oxytetracycline, tetracycline and vancomycin was assessed by disc diffusion method. It was found that 15.3% of isolates were resistant to at least one drug and 36.5% were multidrug resistant. Among isolates, resistance to tetracycline was most commonly encountered (34.6%), followed by resistance to chloramphenicol (25%) and penicillin G (23%). In conclusion, our data also indicate that consuming raw and unpasteurised milk and dairy products could pose a risk of listeriosis in humans. Practical Applications: Although there are plenty of studies about the incidence of L.monocytogenes in raw milk and dairy products in Turkey, our study is the first one in Samsun (Middle Black Sea) Region about Listeria in milk and dairy products. Also in our study, we characterized some virulence genes and serotype distribution of L.monocytogenes by PCR. Finally, we performed antibiotic resistance tests of isolates to see possible public health threats because of using overabundant antibiotics. If we analyze all these work to see the potential risk assessments in this region, our study could be a leading study in near future. © 2016 Wiley Periodicals, Inc.


Utuk A.E.,Veterinary Control Central Research Institute | Piskin F.C.,Veterinary Control Central Research Institute
The Scientific World Journal | Year: 2012

The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey. © 2012 Armagan Erdem Utuk and Fatma Cigdem Piskin.


PubMed | Veterinary Control Central Research Institute
Type: | Journal: TheScientificWorldJournal | Year: 2012

The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey.

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