Veterinary Control and Research Institute

Adana, Turkey

Veterinary Control and Research Institute

Adana, Turkey
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Yapar K.,University of Sfax | Cavusoglu K.,Giresun University | Oruc E.,Veterinary Control and Research Institute | Yalcin E.,Giresun University
Journal of Medicinal Food | Year: 2010

The aim of the present study was to investigate the protective role of Ginkgo biloba leaf extract against uranium (U)-induced toxicity in Swiss albino mice. The mice were randomly divided into six groups, each consisting of six animals: Group I (control) received tap water alone, Group II received U at a dose of 5mg/kg of body weight, Group III received G. biloba at a dose of 50mg/kg of body weight, Group IV received G. biloba at a dose of 150mg/kg of body weight, Group V received G. biloba (50mg/kg of body weight) and U (5mg/kg of body weight), and Group VI received G. biloba (150mg/kg of body weight) and U (5mg/kg of body weight) by oral gavage for 5 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine levels were determined to assess liver and kidney function, respectively. Also, liver and kidney samples were taken for the determination of tissue malondialdehyde (MDA) and reduced glutathione (GSH) levels, and histopathological changes in liver and kidneys were investigated. The results indicated that there was a significant increase (P<.05) in selected serum parameters. Serum AST, ALT, BUN, and creatinine levels significantly increased in mice treated with U alone when compared to the other groups. Moreover, U-induced oxidative damage caused a significant decrease in GSH levels and a significant increase in MDA levels of liver and kidney tissues. Treatment with G. biloba produced amelioration in biochemical indices of hepatotoxicity and nephrotoxicity according to Group II. Each dose of G. biloba provided significant protection against U-induced toxicity, and its strongest effect was observed at a dose of 150mg/kg of body weight. In vivo results showed that G. biloba extract is a potent protector against U-induced toxicity, and its protective role is dose-dependent. © Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2010.


Simsek S.,Firat University | Ciftci A.T.,Veterinary Control and Research Institute
Journal of Arthropod-Borne Diseases | Year: 2016

Background: Dirofilaria immitis and Dirofilaria repens are the most common species of filarial nematodes described in the dogs. A single-step multiplex PCR was applied to detect and differentiate simultaneously and unequivocally D. immitis and D. repens on DNA extracted from canine peripheral blood and besides to detect the seroprevalance of D. immitis by ELISA in Elazig Province, Turkey. A PCR detection of the Wolbachia, which plays an important role in D. immitis biology and contributes to the inflammatory pathology of the heartworm, was also applied for the first time in Turkey. Methods: A total of 161 whole blood and sera samples were collected from stray dogs and stored at -20 °C until used. After DNA extraction, all samples were processed with Dirofilaria primers by multiplex-PCR and Wolbachia primers by conventional PCR besides ELISA for serology. The amplification was performed using a set of primers designed on a portion of the small subunit ribosomal RNA gene of the mitochondrion (12S rDNA). Results: Three of the examined dogs (1.8%) were found to be infected with only D. immitis, one (0.6%) with D. repens and three (1.8%) with both parasites. Besides, 10 out of 161 dogs (6.2%) were found infected with Wolbachia sp. Finaly, the seroprevalence of dirofilariosis in the examined dogs was found to be 3.7% (6/161). Conclusion: Although dirofilariosis is not a serious problem in the region, the stray dogs still continue to be a source of infection.


Albayrak H.,Ondokuz Mayis University | Gumusova S.O.,Ondokuz Mayis University | Ozan E.,Veterinary Control and Research Institute | Yazici Z.,Ondokuz Mayis University
Tropical Animal Health and Production | Year: 2012

All pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep and pigs. Besides the important economical losses, pestiviruses may compromise the normal immune response to other pathogens and increase the severity of other infections in sheep. In this study, aborted foetuses (cattle and sheep) in either coastal or inland Black Sea region of Turkey were surveyed for the presence of RNA from pestiviruses (bovine viral diarrhoea virus (BVDV), border disease virus (BDV)). The presence of BVDV RNA was found in 6 of 21 aborted calves (28.57%), although BDV RNA was detected in 14 of 21 aborted lambs (66.66%) by reverse transcriptase polymerase chain reaction. This study also investigates the distribution of viral RNA within the brain, liver and lung of aborted foetuses. The viral RNA positivity rates for the organs varied and were as follows: brain 40.47% and liver and lung 38.09%. The results revealed that pestiviruses are important abort pathogen in the provinces of northern Turkey. © 2011 Springer Science+Business Media B.V.


Albayrak H.,Ondokuz Mayis University | Ozan E.,Veterinary Control and Research Institute | Kurt M.,Veterinary Control and Research Institute
Tropical Animal Health and Production | Year: 2012

In this study, the hard ticks, whole blood and serum samples collected from small ruminants (sheep and goat) in middle Black Sea region of Turkey where Crimean-Congo hemorrhagic fever (CCHF) human cases were observed in the past years were surveyed for the presence of RNA and specific IgG antibodies from CCFH virus (CCHFV). CCHFV RNA was found in 30 of 255 tick pools (11. 76%) and nine of 105 (8. 57%) leucocyte samples. No CCHFV genomic RNA was detected from animals in Yildizeli and Vezirkopru. However, CCHFV RNA was found from animals in Gerze and Resadiye. Seventy-eight of 105 goat and sheep blood serum samples tested were antibody-positive for CCHFV by enzyme-linked immunosorbent assay (ELISA) (goat: 42/63; sheep: 36/42). Viral RNA was detected from tick samples in all of four provinces. Positivity rates for the provinces varied and were as follows: Gerze 13. 04%, Resadiye 35. 41%, Vezirkopru 1. 61% and Yildizeli 6. 06%. CCHFV genomic RNA was detected in four of seven tick species tested. These results suggest that these hard ticks may act as a reservoir for CCHFV in northern Turkey. © 2012 Springer Science+Business Media B.V.


Albayrak H.,Ondokuz Mayis University | Ozan E.,Veterinary Control and Research Institute | Kurt M.,Veterinary Control and Research Institute
Tropical Animal Health and Production | Year: 2010

Crimean-Congo hemorrhagic fever virus threatens human health. Exposure of the infected tick is a strong risk factor for human disease. In this study, the hard ticks collected from a variety of mammalian species (cattle, sheep, goat, and buffalo) and a turtle in either coastal or inland Black Sea region of Turkey were surveyed for the presence of antigen from Crimean-Congo hemorrhagic fever virus (CCHFV). CCHFV antigen was found in 46 of 421 tick pools (10.92%). Positivity rates for the provinces varied and were as follows: Samsun 33.87%, Ordu 4.34%, Giresun 8.86%, Sinop 6.09%, Amasya 7.40%, Tokat 5.08%, and Sivas 8.06%. CCHFV antigen was detected in seven of 11 tick species tested. These results suggest that these hard ticks may act as a reservoir for CCHFV in northern Turkey. © 2010 Springer Science+Business Media B.V.


Yalcin E.,Giresun University | Oruc E.,Veterinary Control and Research Institute | Cavusoglu K.,Giresun University | Yapar K.,Giresun University
Journal of Medicinal Food | Year: 2010

In this study, the protective role of grape seed extract (GSE) against doxorubicin (DOX)-induced cardiotoxicity and genotoxicity has been evaluated in male Mus musculus var. albino mice. The micronucleus (MN) test in erythrocytes and the chromosome aberration (CA) test in bone marrow cells were used. Also, levels of reduced glutathione (GSH) and lipid peroxidation as malondialdehyde (MDA) in heart homogenates were measured, and in addition the changes in heart histology were investigated. The mice were randomly divided into six groups. Group I (negative control) received intraperitoneal injections of isotonic saline (0.02 mL/g) for 6 consecutive days, Group II received intraperitoneal injections of DOX (2.5 mg/kg of body weight, six doses every other day; cumulative dosage, 15 mg/kg of body weight) for 6 consecutive days, Group III received GSE (50 mg/kg of body weight, 21 doses every other day; cumulative dosage, 1,050 mg/kg of body weight) for 21 consecutive days, Group IV received GSE (150 mg/kg of body weight, 21 doses every other day; cumulative dosage, 3,150 mg/kg of body weight) for 21 consecutive days, Group V received GSE (50 mg/kg of body weight, 28 doses every other day; cumulative dosage, 1,400 mg/kg of body weight) for 28 consecutive days plus DOX (2.5 mg/kg of body weight, six doses every other day; cumulative dosage, 15 mg/kg of body weight) for 6 consecutive days, and Group VI received GSE (150 mg/kg of body weight, 28 doses every other day; cumulative dosage, 4,200 mg/kg of body weight) for 28 consecutive days plus DOX (2.5 mg/kg of body weight, six doses every other day; cumulative dosage, 15 mg/kg of body weight) for 6 consecutive days. DOX induced heart damage as indicated from a pronounced change in heart histology. In the DOX-treated group, there was a significant increase in MDA content in the heart homogenate, and the level of GSH was significantly decreased. DOX induced genotoxicity by increasing the number of aberrant metaphases (AMNs), MNs, and structural chromosomal aberrations (CAs) such as chromatid breaks, dicentrics, acentric fragments, and gaps and showed a detractive effect on the mitotic index (MI) of cells. Pretreatment with GSE before treatment with DOX significantly protected the heart tissue by ameliorating its antioxidant activity. In Groups V and VI, the MDA level of heart tissue was significantly decreased, and the GSH level was increased compared to the DOX-treated group. Moreover, GSE significantly protected bone marrow chromosomes from DOX-induced genotoxicity by reducing the total AMNs and the frequency of structural CAs. GSE treatment also decreased the frequency of MNs and increased the MI values. It could be concluded that GSE acts as a potent antioxidant to prevent heart damage and genotoxicity of bone marrow cells. © Mary Ann Liebert, Inc.


This study investigated the seasonal prevalence and the antimicrobial susceptibility of Campylobacter jejuni and Campylobacter coli in 264 samples of chicken meat. The samples encompassed wings (n=88), breasts (n=79) and thighs (n=97) and were purchased from different butcheries and markets in Elazig province, in Eastern Turkey, between December 2009 and November 2010. The meat samples were tested for Campylobacter presence and the collected isolates were identified as Campylobacter jejuni and Campylobacter coli using polymerase chain reaction (PCR). Resistance rates to 7 antimicrobials were investigated by the disk diffusion method. Campylobacter jejuni was found at a higher prevalence (41.7%) than C. coli (14.4%); C. jejuni was isolated most frequently from breast samples (53.2%) than from thighs (40.2%) and wings (32.9%) samples. The prevalence of C. jejuni and C. coli peaked during the Summer (June-August), with the highest peak occurring in July (77.3%). The lowest prevalence (30%) was detected in February. The prevalence in the Summer (June‑August) was significantly higher (71.2%) than the one reported during the Winter (December‑February) (39.4%, P < 0.05). The highest resistance rate among C. jejuni isolates was observed to tetracycline (38.2%), nalidixic acid (29.1%), and ciprofloxacin (24.5%). Campylobacter coli also showed a high resistance to these antibiotics, although in slightly different proportions: tetracycline (42.1%), ciprofloxacin (31.6%), and nalidixic acid (26.3%). None of the C. jejuni or C. coli isolates was resistant to gentamicin. © 2014, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G.Caporale". All right reserved.


Albayrak H.,Ondokuz Mayis University | Ozan E.,Veterinary Control and Research Institute | Kurt M.,Veterinary Control and Research Institute
Zoonoses and Public Health | Year: 2010

In this study, the hard ticks collected from a variety of mammalian species (cattle, sheep, goat, buffalo) and a turtle in either coastal or inland Black Sea region of Turkey were surveyed for the presence of RNA from Crimean-Congo haemorrhagic fever virus (CCHFV) and West Nile virus (WNV). No WNV genomic RNA was detected in any tick sample. However, CCHFV RNA was found in 29 of 421 tick pools (6.88%). Positivity rates for the provinces varied and were as follows: Samsun 4.38%; Ordu 4.34%; Giresun 6.32%; Sinop 14.63%; Amasya 5.55%; Tokat 3.38% and Sivas 4.83%. Crimean-Congo haemorrhagic fever virus genomic RNA was detected in seven of eleven tick species tested. These results suggest that these hard ticks may act as a reservoir for CCHFV in northern Turkey, but probably have no role in WNV transmission. © 2010 Blackwell Verlag GmbH.


Albayrak H.,Ondokuz Mayis University | Ozan E.,Veterinary Control and Research Institute
Zoonoses and Public Health | Year: 2010

In this study, a total of 402 samples (173 cloacal swab samples, 169 tracheal swab samples and 60 organ pools including the lung, spleen, liver, trachea and brain) obtained from 27 different wild avian species from Kizilirmak delta and the adjacent wetlands in northern Turkey were surveyed for the presence of RNA from Avian influenza virus (AIV) and West Nile virus (WNV) by Taqman-based real-time reverse transcriptase-polymerase chain reaction assay. No WNV genomic RNA was detected in any sample. In contrast, AIV RNA was found in two of 402 samples (0.49%). © 2010 Blackwell Verlag GmbH.


Albayrak H.,Ondokuz Mayis University | Ozan E.,Veterinary Control and Research Institute
Tropical Animal Health and Production | Year: 2010

Equine infectious anaemia is a retroviral infection of horses. All infected horses, including those that are asymptomatic, become carriers and are infectious for life. In this study, blood samples of all equines in the province of Ardahan were collected. The material consisted of 8,947 equines, including 8,769 horses and 178 donkeys, from Ardahan province in northeastern Turkey. Blood was collected from all horses and donkeys and the sera were analysed for the presence of antibodies to equine infectious anaemia virus (EIAV) using an enzyme-linked immunosorbent assay. The results revealed that none of the horses and donkeys were positive for antibodies to EIAV. © 2010 Springer Science+Business Media B.V.

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