Entity

Time filter

Source Type


Albayrak H.,Ondokuz Mayis University | Gumusova S.O.,Ondokuz Mayis University | Ozan E.,Veterinary Control and Research Institute | Yazici Z.,Ondokuz Mayis University
Tropical Animal Health and Production | Year: 2012

All pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep and pigs. Besides the important economical losses, pestiviruses may compromise the normal immune response to other pathogens and increase the severity of other infections in sheep. In this study, aborted foetuses (cattle and sheep) in either coastal or inland Black Sea region of Turkey were surveyed for the presence of RNA from pestiviruses (bovine viral diarrhoea virus (BVDV), border disease virus (BDV)). The presence of BVDV RNA was found in 6 of 21 aborted calves (28.57%), although BDV RNA was detected in 14 of 21 aborted lambs (66.66%) by reverse transcriptase polymerase chain reaction. This study also investigates the distribution of viral RNA within the brain, liver and lung of aborted foetuses. The viral RNA positivity rates for the organs varied and were as follows: brain 40.47% and liver and lung 38.09%. The results revealed that pestiviruses are important abort pathogen in the provinces of northern Turkey. © 2011 Springer Science+Business Media B.V.


Yalcin E.,Giresun University | Oruc E.,Veterinary Control and Research Institute | Cavusoglu K.,Giresun University | Yapar K.,Giresun University
Journal of Medicinal Food | Year: 2010

In this study, the protective role of grape seed extract (GSE) against doxorubicin (DOX)-induced cardiotoxicity and genotoxicity has been evaluated in male Mus musculus var. albino mice. The micronucleus (MN) test in erythrocytes and the chromosome aberration (CA) test in bone marrow cells were used. Also, levels of reduced glutathione (GSH) and lipid peroxidation as malondialdehyde (MDA) in heart homogenates were measured, and in addition the changes in heart histology were investigated. The mice were randomly divided into six groups. Group I (negative control) received intraperitoneal injections of isotonic saline (0.02 mL/g) for 6 consecutive days, Group II received intraperitoneal injections of DOX (2.5 mg/kg of body weight, six doses every other day; cumulative dosage, 15 mg/kg of body weight) for 6 consecutive days, Group III received GSE (50 mg/kg of body weight, 21 doses every other day; cumulative dosage, 1,050 mg/kg of body weight) for 21 consecutive days, Group IV received GSE (150 mg/kg of body weight, 21 doses every other day; cumulative dosage, 3,150 mg/kg of body weight) for 21 consecutive days, Group V received GSE (50 mg/kg of body weight, 28 doses every other day; cumulative dosage, 1,400 mg/kg of body weight) for 28 consecutive days plus DOX (2.5 mg/kg of body weight, six doses every other day; cumulative dosage, 15 mg/kg of body weight) for 6 consecutive days, and Group VI received GSE (150 mg/kg of body weight, 28 doses every other day; cumulative dosage, 4,200 mg/kg of body weight) for 28 consecutive days plus DOX (2.5 mg/kg of body weight, six doses every other day; cumulative dosage, 15 mg/kg of body weight) for 6 consecutive days. DOX induced heart damage as indicated from a pronounced change in heart histology. In the DOX-treated group, there was a significant increase in MDA content in the heart homogenate, and the level of GSH was significantly decreased. DOX induced genotoxicity by increasing the number of aberrant metaphases (AMNs), MNs, and structural chromosomal aberrations (CAs) such as chromatid breaks, dicentrics, acentric fragments, and gaps and showed a detractive effect on the mitotic index (MI) of cells. Pretreatment with GSE before treatment with DOX significantly protected the heart tissue by ameliorating its antioxidant activity. In Groups V and VI, the MDA level of heart tissue was significantly decreased, and the GSH level was increased compared to the DOX-treated group. Moreover, GSE significantly protected bone marrow chromosomes from DOX-induced genotoxicity by reducing the total AMNs and the frequency of structural CAs. GSE treatment also decreased the frequency of MNs and increased the MI values. It could be concluded that GSE acts as a potent antioxidant to prevent heart damage and genotoxicity of bone marrow cells. © Mary Ann Liebert, Inc.


Yapar K.,University of Sfax | Cavusoglu K.,Giresun University | Oruc E.,Veterinary Control and Research Institute | Yalcin E.,Giresun University
Journal of Medicinal Food | Year: 2010

The aim of the present study was to investigate the protective role of Ginkgo biloba leaf extract against uranium (U)-induced toxicity in Swiss albino mice. The mice were randomly divided into six groups, each consisting of six animals: Group I (control) received tap water alone, Group II received U at a dose of 5mg/kg of body weight, Group III received G. biloba at a dose of 50mg/kg of body weight, Group IV received G. biloba at a dose of 150mg/kg of body weight, Group V received G. biloba (50mg/kg of body weight) and U (5mg/kg of body weight), and Group VI received G. biloba (150mg/kg of body weight) and U (5mg/kg of body weight) by oral gavage for 5 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine levels were determined to assess liver and kidney function, respectively. Also, liver and kidney samples were taken for the determination of tissue malondialdehyde (MDA) and reduced glutathione (GSH) levels, and histopathological changes in liver and kidneys were investigated. The results indicated that there was a significant increase (P<.05) in selected serum parameters. Serum AST, ALT, BUN, and creatinine levels significantly increased in mice treated with U alone when compared to the other groups. Moreover, U-induced oxidative damage caused a significant decrease in GSH levels and a significant increase in MDA levels of liver and kidney tissues. Treatment with G. biloba produced amelioration in biochemical indices of hepatotoxicity and nephrotoxicity according to Group II. Each dose of G. biloba provided significant protection against U-induced toxicity, and its strongest effect was observed at a dose of 150mg/kg of body weight. In vivo results showed that G. biloba extract is a potent protector against U-induced toxicity, and its protective role is dose-dependent. © Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2010.


This study investigated the seasonal prevalence and the antimicrobial susceptibility of Campylobacter jejuni and Campylobacter coli in 264 samples of chicken meat. The samples encompassed wings (n=88), breasts (n=79) and thighs (n=97) and were purchased from different butcheries and markets in Elazig province, in Eastern Turkey, between December 2009 and November 2010. The meat samples were tested for Campylobacter presence and the collected isolates were identified as Campylobacter jejuni and Campylobacter coli using polymerase chain reaction (PCR). Resistance rates to 7 antimicrobials were investigated by the disk diffusion method. Campylobacter jejuni was found at a higher prevalence (41.7%) than C. coli (14.4%); C. jejuni was isolated most frequently from breast samples (53.2%) than from thighs (40.2%) and wings (32.9%) samples. The prevalence of C. jejuni and C. coli peaked during the Summer (June-August), with the highest peak occurring in July (77.3%). The lowest prevalence (30%) was detected in February. The prevalence in the Summer (June‑August) was significantly higher (71.2%) than the one reported during the Winter (December‑February) (39.4%, P < 0.05). The highest resistance rate among C. jejuni isolates was observed to tetracycline (38.2%), nalidixic acid (29.1%), and ciprofloxacin (24.5%). Campylobacter coli also showed a high resistance to these antibiotics, although in slightly different proportions: tetracycline (42.1%), ciprofloxacin (31.6%), and nalidixic acid (26.3%). None of the C. jejuni or C. coli isolates was resistant to gentamicin. © 2014, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G.Caporale". All right reserved.


Yildirim N.C.,Tunceli University | Kandemir F.M.,Firat University | Benzer F.,Veterinary Control and Research Institute
Digest Journal of Nanomaterials and Biostructures | Year: 2011

Biological compounds with antioxidant properties may contribute to the protection of cells and tissues against deleterious effects of reactive oxygen species (ROS) and other free radicals induced by cisplatin. This study was performed to investigate the possible protective role of antioxidant treatment with grape seed extract (GSE) on cisplatin-induced testes oxidant injury using biochemical approaches. The degree of protection produced by GSE was evaluated by determining the level of malondialdehyde (MDA) and glutathione (GSH), the activity of catalase (CAT), glutathione peroxidase (GSH-Px), were estimated from testes of rabbits. Male New Zealand white rabbits were divided into 3 groups (n=6):1-Control group(1 ml saline. i.p) 2-Cisplatin group (a single dose of cisplatin (5 mg/kg, i.p) 3- A single dose of cisplatin (5 mg/kg, i.p) + GSE (250 mg/kg/day, i.p) for 6 days. MDA level were significantly higher when compared to Control (p<0.05). In Cisplatin+grapes groups MDA levels were found significantly lower than cisplatin group (p<0.05). GSH levels were decreased with cisplatin compared to control but in case of cisplatin+ GSE it increased (p<0.05). In treated rabbits, the activity of CAT and GSH-Px was decreased in cisplatin and cisplatin+ GSE groups compared to control (p<0.001). These results indicate that the antioxidant GSE might have a protective effect against cisplatin-induced testiculer damage and oxidative stress in rabbit.

Discover hidden collaborations