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Bangalore, India

Kanafi M.M.,Manipal University India | Rajeshwari Y.B.,Veterinary College KVAFSU | Gupta S.,M. S. University of Baroda | Dadheech N.,M. S. University of Baroda | And 3 more authors.
Cytotherapy | Year: 2013

Background aims: The success of islet transplantation for diabetes depends on the availability of an adequate number of allogeneic or autologous islets. Postnatal stem cells are now considered for the generation of physiologically competent, insulin-producing cells. Our group showed earlier that it is possible to generate functional islets from human dental pulp stem cells by using a serum-free cocktail in a three-step protocol. Methods: We compared the yield of generated islet-like cell clusters (ICCs) from stem cells from pulps of human exfoliated deciduous teeth (SHED) and dental pulp stem cells from permanent teeth (DPSCs). ICCs derived from SHED were packed in immuno-isolatory biocompatible macro-capsules and transplanted into streptozotocin (STZ)-induced diabetic mice. Non-diabetic and diabetic controls were transplanted with macro-capsules with or without islets. Results: SHED were superior to DPSCs. STZ diabetic mice alone and mice transplanted with empty macro-capsules exhibited hyperglycemia throughout the experiment, whereas mice transplanted with macro-capsules containing ICCs were restored to normoglycemia within 3-4 weeks, which persisted for >60 days. Conclusions: Our results demonstrate for the first time that ICCs derived from SHED reverse STZ diabetes in mice without immunosuppression and offer an autologous and non-controversial source of human tissue that could be used for stem cell therapy in diabetes. © 2013 International Society for Cellular Therapy.

Balamurugan V.,Project Directorate on Animal Disease Monitoring and Surveillance | Krishnamoorthy P.,Project Directorate on Animal Disease Monitoring and Surveillance | Veeregowda B.M.,Veterinary College KVAFSU | Sen A.,Indian Veterinary Research Institute | And 4 more authors.
Tropical Animal Health and Production | Year: 2012

This study describes seroprevalence of Peste des petits ruminants (PPR) in cattle and buffaloes carried out during the period 2009-2010 using the randomly collected serum samples from different parts of Southern peninsular India. The report presents the results of PPR virus (PPRV)-specific antibodies in situations where either the subclinical or inapparent or non-lethal infection was there in cattle and buffaloes. A total of 2,548 serum samples [cattle = 1,158, buffaloes = 1,001, sheep = 303 and goat = 86] were collected and screened for PPRV antibodies by using a PPR monoclonal antibody-based competitive ELISA kit. Analysis of 2,159 serum samples indicates an overall 4. 58% prevalence of PPRV antibody in cattle and buffaloes. The presence of PPRV-specific antibodies demonstrates that cattle and buffaloes are exposed to PPR infection naturally, and the transmission mode may be direct or indirect. Further, it implies the importance of bovines as subclinical hosts for the virus besides widespread presence of the disease in sheep and goats in the country. © 2011 Springer Science+Business Media B.V.

Murthy H.N.N.,Veterinary College KVAFSU | Shome R.,Animal Disease Monitoring and Surveillance PD ADMAS | Suryanarayana V.V.S.,Indian Veterinary Research Institute
Indian Journal of Biotechnology | Year: 2013

Bovine mastitis is the most important source of loss for the growing dairy industry. Streptococci, with special reference to Streptococcus agalactiae, S. dysgalactiae and S. uberis, are the predominant pathogens causing bovine mastitis. A rapid, sensitive and specific test for the detection of these pathogens needs to be developed. To accomplish this, initially 163 milk samples were collected from various organized and unorganized sectors in and around Bangalore, India. These milk samples were screened for subclinical mastitis by somatic cell counting (SSC) and electro conduction (EC). Of these, 131 samples selected based on SCC and EC values were subjected for isolation of the organisms. Two sets of specific primers, targeting streptococcal 16S rRNA gene were designed for detection of S. agalactiae, S. dysgalactiae and S. uberis. The results of the study showed S. agalactiae as the predominant streptococci among the generally identified streptococcal species associated with subclinical bovine mastitis in dairy cattle in and around Bangalore.

Senthilnathan G.,Veterinary College KVAFSU | Shenbagam S.,Veterinary College KVAFSU | Suryanarayana T.,Veterinary College KVAFSU | Thiyageeswaran M.,Veterinary College KVAFSU
Indian Journal of Animal Research | Year: 2015

The presence of pathogenic mycoplasma species in the population of respiratory disease suspected broiler breeder birds in two commercial poultry farms of Tamil Nadu was investigated. A total of 144 samples were collected from suspected rearing breeder birds and examined by culture and species-specific polymerase chain reaction (PCR) assay targeting 16S rRNA of Mycoplasma synoviae (MS). 28 samples were found unfit due to high growth of contaminants and hence discarded. The samples showing whirlpool growth in liquid medium (suggestive of mycoplasma) were then inoculated on to solid medium and incubated anaerobically. 42 out of 116 (36.2%) samples showed whirlpool growth and were streaked on to solid medium, however “fried egg” like micro colonies of mycoplasma was evidenced in only 18 samples (15.5%) on day 5 after inoculation. The samples left after discarding the contaminated tubes were subjected to PCR with species specific primer of M. Synoviae. 57 out of 116 samples (49.1%) showed positivity to 207 bp (16s rRNA) product of MS. The nucleotide analysis of amplicon showed 96% homology with reference strain by BLAST analysis. This study revealed that MS infection represents a very serious issue to be considered in rearing broiler breeder birds. © 2014, Indian Journal of Animal Research. All Rights Reserved.

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