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Bangalore, India

He L.,University of Minnesota | Lamont E.,University of Minnesota | Veeregowda B.,Veterinary College Hebbal | Sreevatsan S.,University of Minnesota | And 3 more authors.
Chemical Science | Year: 2011

A "two-step" aptamer-based surface-enhanced Raman scattering (SERS) detection assay was developed for ricin in liquid foods. Ricin B chain was first captured out of food matrices by aptamer-conjugated silver dendrites and then the spectrum was directly read on the silver dendrites. Aptamer use in this assay promotes ease of manipulation as well as improved sensitivity compared to antibody-based approaches. The limit of detection for ricin B chain was 10 ng mL-1 in phosphate buffered saline (PBS), 50 ng mL -1 in orange juice, and 100 ng mL-1 in milk based on principal component analysis (PCA) of measured spectra. This assay shows great promise as a rapid (< 40 min), sensitive, and simple "Yes/No" method to detect bio-weapons like ricin in liquid foods. © The Royal Society of Chemistry 2011. Source

Mudasir Ayoub S.,Veterinary College Hebbal | Rao S.,Veterinary College Hebbal | Munivenkatappa Byregowda S.,Institute of Animal Health and Veterinary Biologicals | Laxmikanth Satyanarayana M.,Veterinary College Hebbal | And 3 more authors.
Brazilian Journal of Veterinary Pathology | Year: 2013

Momordica charantia or Bitter Melon, a tropical vegetable, is a common food in Indian cuisine and has been used extensively in folk medicine as a remedy for diabetes. The present study was undertaken to evaluate the hypoglycemic effect of Momordica charantia extract in streptozotocin-induced diabetic rat model for a period of 45 days. The alcoholic extract of Momordica charantia was administered orally at the dose rate of 100 and 200 mg/kg body weight in distilled water and compared with standard oral hypoglycemic drug, glibenclamide. In the study a significant (P≤ 0.001) improvement in the physiological and biochemical parameters such as body weight, hemoglobin concentration, serum glucose, cholesterol and triglyceride levels was observed in Momordica charantia treated rats as compared to diabetic control rats. In Momordica charantia treated rats there was gradual and progressive alleviation of streptozotocin effects with M. charantia at higher dose rate (200 mg per kg body weight), more effective in normalizing the pancreatic endocrinal architecture, improving the number of β-cells and in enhancing the insulin secretion. Immunohistochemistry and special staining revealed improvement in the insulin secretion in Momordica charantia and glibenclamide treated groups. Source

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