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Bad Langensalza, Germany

Klimowicz-Bodys M.D.,Veterinary Clinic | Klimowicz-Bodys M.D.,Agricultural University of Krakow | Batkowski F.,Wroclaw University | Ochrem A.S.,Agricultural University of Krakow | Savic M.A.,IMV Technologies ZI Est de lAigle
Theriogenology | Year: 2012

The aim of these experiments was to compare the conventional, microscopic method of evaluating pigeon sperm viability to sperm assessed by flow cytometry. Semen was collected twice a week from two groups of pigeons. In every group were 20 males (Group I: meat-type breed; Group II: fancy pigeon breed). Semen was collected using the lumbosacral and cloacal region massage method. Ejaculates collected from each group were pooled and diluted to 10 × 10 6 sperm/ml in BPSE solution. Samples were divided into three equal parts and estimated after collection as well as after in vitro storage for 3, 6 and 24 h. The first part was using for semen motility evaluation. The proportion of motile spermatozoa (MOT) and progressive movement (PMOT) of fresh and stored semen were evaluated using the CASA-system. The second part was examined subjectively by microscope (eosin-nigrosin (EN), eosin-nigrosin staining), the third one was assessed using dual fluorescence SYBR-14/propidium iodide (PI) and flow cytometry (FC). There were not any significant differences in sperm viability and motility between the groups at 0, 3, 6, and 24 h post collection. The percentage of viable spermatozoa in fresh semen determined by EN and FC was not different in Groups I and II (I - 88.71 ± 5.42 and 84.01 ± 3.19, respectively; II-90.87 ± 6.01 and 87.38 ± 5.57, respectively). Significantly lower percentages of viable spermatozoa were detected by FC compared to the EN method in both groups after 6 h (P ≤ 0.05) as well as 24 h (P ≤ 0.01) of storage. Moreover, the dual fluorescent SYBR-14/PI staining allowed for the identification a third population of double stained, moribund spermatozoa. High positive correlations in percentage of live spermatozoa were noted between EN and FC methods in both groups of birds. Evaluation of sperm viability by FC is a rapid, accurate, sensitive, and objective method for the assessment of pigeon sperm viability in fresh as well as stored semen. © 2012 Elsevier Inc.

Kilar P.,Veterinary Clinic
Wiadomości parazytologiczne | Year: 2011

Domestic dogs in Poland are attacked by five species of ticks. The present study shows results of research on species of ticks, their intensity and prevalence of infection in the domestic dogs in the district of Rymanów (Subcarpathian province, Poland). Local research was conducted from 25 August 2005 to 27 October 2006 on 82 randomly selected dogs in the veterinarian centre in Rymanów. Collected ticks were put into tubes with 70% ethyl alcohol. Collected and full up ticks were examined straight after being taken out of the preserving liquid. Stereoscopic microscope for species identification was used. The study revealed that domestic dogs in the Rymanów district are hosts for two species of ticks from the Ixodidae family: Ixodes (Ixodes) ricinus (Linnaeus, 1758) and Ixodes (Pholeoixodes) hexagonus Leach, 1815. Only one dog was infected with both tick species simultaneously. The dogs were attacked mainly by females. Ticks usually attached to such places as the neck, ear conches or groin. In general, the prevalence of ticks infestation on domestic dogs was 48.78%; I. ricinus prevalence was 47.56%, whereas I. hexagonus--2.43%. On the basis of obtained results it can be claimed that I. ricinus is more prevalent in the Rymanów district than I. hexagonus, therefore the domestic dogs are more frequently attacked by this species of ticks.

Lefkaditis M.,Laboratory of Microbiology and Parasitology | Koukeri S.,Veterinary Clinic | Cozma V.,University of Veterinary Medicine
Helminthologia | Year: 2010

Summary: Following an examination conducted on a population of three hundred and forty-one dogs living on the eastern foothills of Mt Olympus in Northern Greece, 17.9 % were found to be seropositive for the Dirofilaria immitis specific antigen. These results indicate that the region is an endemic area for the above-mentioned parasitosis in dogs. Serum samples were examined using the PetChek kit Snap (IDDEX Laboratories, ELISA, Portland USA) according to the manufacturer's instructions. With respect to gender, age and type of coat, the statistical analysis has shown that the prevalence of seropositive results is significantly higher (P < 0.05) among male dogs, older dogs and short-haired dogs. As regards breed and size, the same study has recorded a higher prevalence in mixed breed and small-sized dogs. © 2010 Parasitological Institute of SAS.

Hornok S.,Szent Istvan University | Mester A.,Veterinary Clinic | Takacs N.,Szent Istvan University | Fernandez De Mera I.G.,Institute Investigacion en Recursos Cinegeticos IREC | And 3 more authors.
Parasites and Vectors | Year: 2014

Background: The prevalence of bovine babesiosis caused by Babesia divergens has been declining during the past decades in northeastern Hungary, and no cases have been observed since 2008. Infections of cattle with B. major and Theileria buffeli were hitherto reported in southern and western Europe. In other parts of the globe, there is evidence of emergence and a growing clinical importance of T. buffeli and closely related genotypes of the T. orientalis complex. Findings: In a herd of 88 beef cattle kept in northeastern Hungary, bovine piroplasmosis was diagnosed in nine animals through the examination of blood smears or by molecular methods. B. major was identified in five animals, two of which died. In addition, four cattle harboured T. buffeli, and one of these animals was anaemic. Despite their presence, a contributory role of Anaplasma marginale and A. phagocytophilum could not be established in the disease cases. Conclusions: In this study B. major and bovine theileriosis is reported for the first time in central-eastern Europe, where clinical cases were associated with a mild winter. © 2014 Hornok et al.; licensee BioMed Central Ltd.

Hornok S.,Szent Istvan University | Mester A.,Veterinary Clinic | Takacs N.,Szent Istvan University | Baska F.,Szent Istvan University | And 7 more authors.
Parasites and Vectors | Year: 2015

Background: Reports on Sarcocystis-infection of cattle are outdated or lacking in many European countries, including those in the Central-Eastern part of the continent. Therefore, to assess the prevalence of Sarcocystis spp. among bovids in Hungary, a countrywide survey was initiated. In addition, fulminant deaths of four cattle, that showed clinical signs and post mortem lesions resembling acute sarcocystiosis ("Dalmeny disease"), were investigated. Methods: During the countrywide survey individual heart and oesophagus samples were collected at slaughterhouses from 151 beef cattle and from 15 buffalo, kept in 31 places of Hungary. Analysis for Sarcocystis spp. was carried out with conventional PCRs for the 18S rDNA gene and gel electrophoresis, followed by sequencing of 36 strongly positive samples. Mortality cases were evaluated by histological, molecular, bacteriological and virological analyses of samples from various organs. Results: Among slaughtered cattle the rate of Sarcocystis-infection was 66%. S. cruzi was identified as the most prevalent species in aurochs-like breed, and the zoonotic S. hominis in Hungarian grey cattle. Concerning the sudden deaths of cattle, Sarcocystis-infection could not be demonstrated in organs showing haemorrhages, but S. cruzi cysts were present in the muscles. In one case "S. sinensis" was molecularly identified in the blood (indicating sarcocystaemia). Results of analyses for bacterial/viral pathogens were negative. Conclusions: S. cruzi appears to be the most prevalent Sarcocystis sp. in cattle in Hungary, followed by the zoonotic S. hominis. However, the rate of infection with both species was shown to differ between cattle breeds. The suspected role of Sarcocystis spp. as causative agents of the fatal cases could not be confirmed. © 2015 Hornok et al.; licensee BioMed Central.

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