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Pan M.L.,National Chi Nan University | Lin W.Y.,Taichung Veterans General Hospital Taichung | Lin W.Y.,Central Taiwan University of Science and Technology | Lin W.Y.,China Medical University at Taichung | And 6 more authors.
Journal of Analytical Chemistry | Year: 2014

We conducted a clinical trial to analyze human serum containing carbamazepine by using the differential pulse voltammetry (DPV) method with a glassy carbon electrode, and compared it with the fluorescence polarization immunoassay (FPIA). Thirty patients, who visited our hospital to have their serum carbamazepine level checked, were enrolled. Ten mL of venous blood was collected from each patient and analyzed by DPV and FPIA methods. The correlation between the carbamazepine concentrations determined by DPV and FPIA was good, with an RSQ of 0.998. The similarity of the results indicates that these two methods can be used interchangeably. The DPV method using a glassy carbon electrode may be a potential alternative method to determine the carbamazepine level in human serum. © 2014 Pleiades Publishing, Ltd.

PubMed | Chang Gung University, Taichung Veterans General Hospital Taichung, Chung Shan Medical University, Tzu Chi University and 3 more.
Type: Journal Article | Journal: International journal of clinical and experimental pathology | Year: 2014

Epstein-Barr Virus (EBV) is a herpesvirus usually infecting B-cells but may occasionally infect T- or natural killer (NK)-cells. EBV-associated T- or NK-cell lymphoproliferations represent a continuous spectrum of diseases ranging from asymptomatic infection, infectious mononucleosis (IM), to clonal and malignant lymphoproliferations including systemic EBV-positive T/NK-cell lymphoproliferative disease (EBV-T/NK-LPD) of childhood and hydroa-vacciniforme-like lymphoma of the skin. The clonal diseases are more prevalent in East Asia and exhibit overlapping clinical and pathological features with chronic active EBV infection. Here we report our experience on 10 cases of EBV-associated T-cell lymphoproliferation from Taiwan including five males and five females with a median age of 18 years old (range, 15-28). The most common clinical symptoms were fever, neck mass and hepatosplenomegaly. Eight of these patients showed elevated lactate dehydrogenase level and half of the patients had cytopenia. All patients had either elevated EBV antibody titers or increased serum EBV DNA levels. Five cases were clinically IM-like with polyclonal (3 cases) or clonal (2 cases) T-cell lymphoproliferation. Two patients each had chronic active EBV infection (CAEBV) and hemophagocytic lymphohistiocytosis (HLH). One patient had both CAEBV and HLH. One of the HLH patients with marrow infiltration by intra-sinusoidal large atypical lymphocytes experienced a fulminant course. In a median follow-up time of 21.5 months, seven patients were free of disease, one was alive with disease, and two died of disease in 31 and 3 months, respectively, despite chemotherapy. We confirmed a wide clinicopathological range of EVB-associated T-cell lymphoproliferation in Taiwan. Furthermore, monomorphic LPD and the single case with fulminant course as defined by Ohshima et al (Pathol Int 2018) as categories A3 and B, respectively, died of disease despite chemotherapy. Our report, the largest series in the recent decade from Taiwan, adds to the understanding of these rare diseases with variable clinical and histopathological presentations.

Wu R.-J.,Providence University | Wu R.-J.,The Interdisciplinary Center | Tsai T.-C.,Providence University | Luk H.-N.,Providence University | And 3 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2011

Fast response electrochemical impedance (EI) method was developed to detect concentrations of local anesthetic Levobupivacaine. It revealed the EI method possessed fast response and recovery times and the lowest detected concentration was 1 ppm. Pyrrole was electrochemically polymerized to polypyrrole and made a composite with single walled carbon nanotubes coated over gold electrodes for sensing studies. Ppy and Ppy/SWCNT composite materials were coated upon Au electrodes and characterized by UV/Vis, Fourier Transform Infrared (FTIR), Cyclic Voltammetry (CV) and Transmission Electron Microscope (TEM). Various concentrations of levobupivacaine in the range, 1 to 500 ppm were prepared in medically significant saline solution of 0.9% NaCl as test samples. A 10-kHz frequency was used for the calibration curve, and the short response and recovery time were tested as 5 s and 3 s, respectively. The Ppy/SWCNT material with R2 as 0.9971 showed better linearity than Ppy material. Using molecular dynamic simulation studies exothermic adsorption energies and bond lengths have been calculated and explained the fast response time and lower impedance of Ppy/SWCNT than Ppy. Copyright © 2011 American Scientific Publishers.

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