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Marcy, France

Braun J.P.,National Veterinary School of Toulouse | Trumel C.,National Veterinary School of Toulouse | Bezille P.,VetAgroSup
Small Ruminant Research | Year: 2010

As in other species, the first point in sheep clinical biochemistry is the correct selection of the appropriate tests and, consequently, the optimal management of the pre-analytical phase from the collection of the samples to their management and possible transport or storage before analysis. There are so many different breeds and breeding systems in sheep, as well as laboratory techniques, that no universally acceptable reference values and ranges can be provided. Each laboratory should determine its own reference values and ranges, according to recommended methods. The main uses of clinical biochemistry in sheep health management are in the diagnosis of liver, muscle and nutritional disorders, for which selected examples are discussed in this paper. © 2010 Elsevier B.V. Source


The comparison of the coherence and effectiveness of the action of the Local Centres of Information and Gerontological Coordination (CLIC in French) in these two ageing rural départements highlighted certain strong conclusions giving food for thought regarding public action. First of all the bottom-up and top-down CLIC's both possess different assets. Whereas the former manage to mobilise local actors easily, the latter find this more difficult. The situation is reversed regarding partnerships with institutional actors. Coordination resulting from ascending measures leads to a more complete grid of their territory, especially concerning their target of potentially dependent people over the age of 75. Lastly, the frequent criticism that the CLIC's have a high level of transaction costs seems all the more unjustified as they are long-established and have shown that they are able to provide the necessary training. Source


Sahar A.,VetAgroSup | Sahar A.,National University of Sciences and Technology | Dufour T.,VetAgroSup
LWT - Food Science and Technology | Year: 2014

In this research work we explored the potential of mid infrared (MIR) spectroscopy to determine spoilage microorganism on the surface of chicken breast fillets that were kept aerobically at 5°C for 0, 1, 2, 3, 5 and 8 days, and at 15°C for 0, 0.5, 1, 2, 3 and 5days. It is shown that MIR spectroscopy (4000 - 1000cm-1 range) coupled with attenuated total reflectance (ATR) accessory can be used directly on the surface of meat samples to produce fingerprints. Culture dependent methods were used to determine total viable count (TVC), Pseudomonas, Enterobacteriaceae and Brochothrix thermosphacta on chicken breast fillets at each step of the 2 kinetics. In parallel, MIR spectra were recorded. For each kinetic, partial least square discriminant analysis (PLSDA) results showed 100% of good classifications for the six investigated storage times using 4 PLS factors. PLS regression was carried out to predict the microbial counts from the MIR spectral data. Using PLS model with four factors, good correlation (R2=0.99) and very small root mean squares error of validation (between 0.01 and 0.97log cfu/cm2) showed a strong correlation between MIR spectral data sets and the results obtained using traditional methods. © 2013 Elsevier Ltd. Source


Carlier J.,Laboratoire LAT LUMTOX | Carlier J.,University Claude Bernard Lyon 1 | Guitton J.,Laboratoire Of Toxicologie | Guitton J.,Center Hospitalier Lyon Sud | And 7 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

Hypoglycin A (HGA) is the toxic principle in ackee (Blighia sapida Koenig), a nutritious and readily available fruit which is a staple of the Jamaican working-class and rural population. The aril of the unripe fruit has high concentrations of HGA, the cause of Jamaican vomiting sickness, which is very often fatal. HGA is also present in the samara of several species of maple (Acer spp.) which are suspected to cause seasonal pasture myopathy in North America and equine atypical myopathy in Europe, often fatal for horses. The aim of this study was to develop a method for quantifying HGA in blood that would be sensitive enough to provide toxicological evidence of ackee or maple poisoning. Analysis was carried out using solid-phase extraction (HILIC cartridges), dansyl derivatization and UHPLC-HRMS/MS detection. The method was validated in whole blood with a detection limit of 0.35. μg/L (range: 0.8-500. μg/L). This is the first method applicable in forensic toxicology for quantifying HGA in whole blood. HGA was quantified in two serum samples from horses suffering from atypical myopathy. The concentrations were 446.9 and 87.8. μg/L. HGA was also quantified in dried arils of unripe ackee fruit (Suriname) and seeds of sycamore maple (Acer pseudoplatanus L.) (France). The concentrations were 7.2 and 0.74. mg/g respectively. © 2014 Elsevier B.V. Source


Glardon O.-J.,University of Bern | Pache V.,Cabinet veterinaire des Jordils | Magnenat A.-L.,University of Bern | Pin D.,VetAgroSup | Parvis A.,Cabinet veterinaire des Jordils
Schweizer Archiv fur Tierheilkunde | Year: 2014

In this retrospective study, the tolerance to subcutaneus mistletoe injections (Viscum album L.), adverse reactions and possible indications have been evaluated in feline patients of a small animal clinic. Among the 22 cats treated between 2008 and 2013, 4 did not accept the injections done by the owner, 7 showed slight short time adverse reactions, that disappeared spontaneously. No long term (more than 70 days) adverse reaction directly related to the Viscum albumtreatment could be identified. This study shows that Iscador® can be injected subcutaneously without a risk of worsening of the clinical signs or exacerbation of tumors. The antitumoral, but also immune-modulating and antiinflammatory properties offer interesting treatment opportunities for dermatologic, odonto-stomatologic or allergic patients. © 2014 Verlag Hans Huber, Hogrefe AG, Bern. Source

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