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Magdaleno-Mendez A.,University of Veracruz | Dominguez B.,University of Veracruz | Rodriguez-Andrade A.,Veracruz Institute of Technology | Barrientos-Morales M.,University of Veracruz | And 5 more authors.
Endocrine | Year: 2015

A variety of ion channels are expressed in the plasma membrane of somatotropes within the anterior pituitary gland. Modification of these channels is linked to intracellular Ca2+ levels and therefore to hormone secretion. Previous investigations have shown that the gut-derived orexigenic peptide hormone ghrelin and synthetic GH-releasing peptides (GHRPs) stimulate release of growth hormone (GH) and increase the number of functional voltage-gated Ca2+ and Na+ channels in the membrane of clonal GC somatotropes. Here, we reveal that chronic treatment with ghrelin and its synthetic analog GHRP-6 also increases GH release from bovine pituitary somatotropes in culture, and that this action is associated with a significant increase in Na+ macroscopic current. Consistent with this, Na+ current blockade with tetrodotoxin (TTX) abolished the ghrelin- and GHRP-6-induced increase in GH release. Furthermore, semi-quantitative and real-time RT-PCR analysis revealed an upregulation in the transcript levels of GH, as well as of NaV1.1 and NaV1.2, two isoforms of TTX-sensitive Na+ channels expressed in somatotropes, after treatment with ghrelin or GHRP-6. These findings improve our knowledge on (i) the cellular mechanisms involved in the control of GH secretion, (ii) the molecular diversity of Na+ channels in pituitary somatotropes, and (iii) the regulation of GH and Na+ channel gene expression by ghrelin and GHRPs. © 2014, Springer Science+Business Media New York. Source


Carvajal-Zarrabal O.,University of Veracruz | Nolasco-Hipolito C.,University Malaysia Sarawak | Barradas-Dermitz D.M.,Veracruz Institute of Technology | Hayward-Jones P.M.,University of Veracruz | And 2 more authors.
Journal of Environmental Management | Year: 2012

Vinasse, the wastewater from ethanol distillation, is characterised by high levels of organic and inorganic matter, high exit process temperature (ca. 90°C) and low pH (3.0-4.5). In this study, the treatment of tequila vinasse was achieved by a flocculation-coagulation process using poly-γ-glutamic acid (PGA). Results showed that the use of PGA (250-300. ppm) combined with sodium hypochlorite and sand filtration managed to remove about 70% of the turbidity and reduced chemical oxygen demand (COD) by 79.5% with the extra benefit of colour removal. PGA showed its best flocculating activity at pH 2.5-3.5 and a temperature of 30-55°C. Such a treatment may be a solution for small tequila companies for which other solutions to deal with their vinasse may not be economically affordable. © 2011 Elsevier Ltd. Source


Carvajal-Zarrabal O.,University of Veracruz | Nolasco-Hipolito C.,University Malaysia Sarawak | Aguilar-Uscanga M.G.,Veracruz Institute of Technology | Melo Santiesteban G.,University of Veracruz | And 2 more authors.
BioMed Research International | Year: 2014

Metabolic changes, along with cardiovascular and hepatic factors, are associated with the development of diseases such as diabetes, dyslipidemia, and obesity. We evaluated the effect of avocado oil supplementation (centrifuged and solvent extracted), compared with olive oil, upon the hepatic function in sucrose-fed rats. Twenty-five rats were divided into five groups: control (basal diet), a sucrose-fed group (basal diet plus 30% sucrose solution), and three other groups (S-OO, S-AOC, and S-AOS, indicating basal diet plus 30% sucrose solution plus olive oil OO, avocado oil extracted by centrifugation AOC or using solvent AOS, resp.). Glucose, total cholesterol, triglycerides, total protein, albumin, globulin, direct bilirubin, glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, alkaline phosphatase, cholinesterase, and α-amylase concentrations were determined and avocado oil effect on them was studied. In some cases the induced metabolic alteration significantly affected total protein and bilirubin levels and also had a highly significant effect on α-amylase levels. AOC and AOS exhibited effects similar to those of olive oil, according to the nonsignificant difference in fatty acid profile observed by other authors. Avocado oil consumption could be beneficial in the control of altered metabolic profile illnesses as it presents effects on hepatic function biochemical markers similar to olive oil. © 2014 Octavio Carvajal-Zarrabal et al. Source


Carvajal-Zarrabal O.,University of Veracruz | Nolasco-Hipolito C.,University Malaysia Sarawak | Aguilar-Uscanga M.G.,Veracruz Institute of Technology | Melo-Santiesteban G.,University of Veracruz | And 2 more authors.
Disease Markers | Year: 2014

The purpose of this study was to evaluate the effects of avocado oil administration on biochemical markers of cardiovascular risk profile in rats with metabolic changes induced by sucrose ingestion. Twenty-five rats were divided into five groups: a control group (CG; basic diet), a sick group (MC; basic diet plus 30% sucrose solution), and three other groups (MCao, MCac, and MCas; basic diet plus 30% sucrose solution plus olive oil and avocado oil extracted by centrifugation or using solvent, resp.). Glucose, total cholesterol, triglycerides, phospholipids, low- and high-density lipoproteins (LDL, HDL), very low-density lipoprotein (VLDL), lactic dehydrogenase, creatine kinase, and high sensitivity C-reactive protein concentration were analyzed. Avocado oil reduces TG, VLDL, and LDL levels, in the LDL case significantly so, without affecting HDL levels. An effect was exhibited by avocado oil similar to olive oil, with no significant difference between avocado oil extracted either by centrifugation or solvent in myocardial injury biochemical indicators. Avocado oil decreased hs-CRP levels, indicating that inflammatory processes were partially reversed. These findings suggested that avocado oil supplementation has a positive health outcome because it reduces inflammatory events and produces positive changes in the biochemical indicators studied, related to the development of metabolic syndrome. © 2014 Octavio Carvajal-Zarrabal et al. Source


Herrera-Lee R.G.,Veracruz Institute of Technology | Herrera-Lee R.G.,University of Veracruz | Rios-Figueroa H.V.,University of Veracruz | Nakano T.,University of Alberta | And 4 more authors.
Milchwissenschaft | Year: 2012

Previous studies have provided evidence of a women's better accuracy in interpreting emotional states, which makes them expert judges interpret infant facial expressions. However, little information is available if balanced hedonic scales really describe babies' enjoyment of food when an evaluation is only based on the ability to interpret infant facial expressions while consumption of specific food occurs. This might play an important role in our knowledge about food preferences at a young age. In the present study, we explored adults' hedonic perception based affective expressions from babies 4- to 6-month-old while drinking an infant formula. Expert judges evaluated babies' photographs. The analysis of babies' photographs suggested that expert adult panelists perceived babies' enjoyment of the infant formulas within three basic categories, one "positive", one "weakly negative", and one "strongly negative". This classification suggests that enjoyment of an infant formula provokes emotions in babies that are either "positive" or "negative", but not "neutral". Therefore, it is suggested that more objective measuring methods should be developed. Source

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