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Wu Y.,Hunan Vegetable Research Institute | Zhang Z.,Hunan Vegetable Research Institute | Zhou X.,Hunan Vegetable Research Institute | Chao J.,Xiangxi Autonomous Prefecture Tobacco Company | And 5 more authors.
Acta Tabacaria Sinica | Year: 2014

Mutant giant strains of Yunyan 87 dormant buds were tested for breaking of dormancy to germination, callus induction, shoot proliferation and rooting cultivation. Results showed that: the optimum medium for breaking dormancy to germination was MS+2.0 mg/L 6-BA+1.0 mg/L NAA; the optimum medium for callus induction was MS+3.0mg/L 6-BA+0.3mg/L NAA; the medium with optimum shoot proliferation effect was MS+1.0mg/L 6-BA+0.3mg/L NAA; MS+0.6mg/L NAA was the best condition for root growth. ©, 2014, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.

Kong Q.,Hunan Vegetable Research Institute | Kong Q.,Huazhong Agricultural University | Zhang G.,Hunan Vegetable Research Institute | Zhang G.,Central South University | And 4 more authors.
American Journal of Botany | Year: 2012

Premise of the study: The redundancies in expressed sequence tags (ESTs) in the National Center for Biotechnology Information sequence database were used to identify and develop polymorphic simple sequence repeat (SSR) markers for pepper (Capsicum annuum). Methods and Results: Sixty-eight polymorphic SSR loci were identified in the contigs (containing redundant ESTs) generated by assembling 118 060 pepper ESTs from the public sequence database. Thirty-three SSR markers exhibited polymorphism among 31 pepper varieties, with alleles per SSR marker ranging from two to six. The mean observed and expected heterozygosity were 0.28 and 0.39, respectively. There were 18 SSR markers with a motif repeat number of less than five, accounting for 55% of the total. Conclusions: We demonstrated the value of mining the redundant sequences in public sequence databases for the development of polymorphic SSR markers, which can be used for marker-assisted breeding in pepper. © 2012 Botanical Society of America.

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