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Liu Z.,Hunan University | Kang L.,Hunan University | Liu Y.,Hunan University | Lv J.,Hunan University | And 17 more authors.
Gene | Year: 2017

MicroRNAs (miRNAs) are non-coding small RNAs which play an important regulatory role in various biological processes. Previous studies have reported that miRNAs are involved in fruit development in model plants. However, the miRNAs related to fruit development and quality in hot pepper (Capsicum annuum L.) remains unknown. In this study, small RNA populations from different fruit ripening stages and different varieties were compared using next-generation sequencing technology. Totally, 59 known miRNAs and 310 novel miRNAs were identified from four libraries using miRDeep2 software. For these novel miRNAs, 656 targets were predicted and 402 of them were annotated. GO analysis and KEGG pathways suggested that some of the predicted miRNAs targeted genes involved in starch sucrose metabolism and amino sugar as well as nucleotide sugar metabolism. Quantitative RT-PCR validated the contrasting expression patterns between several miRNAs and their target genes. These results will provide an important foundation for future studies on the regulation of miRNAs involved in fruit development and quality. © 2017 Elsevier B.V.


Wu Y.,Hunan Vegetable Research Institute | Zhang Z.,Hunan Vegetable Research Institute | Zhou X.,Hunan Vegetable Research Institute | Chao J.,Xiangxi Autonomous Prefecture Tobacco Company | And 5 more authors.
Acta Tabacaria Sinica | Year: 2014

Mutant giant strains of Yunyan 87 dormant buds were tested for breaking of dormancy to germination, callus induction, shoot proliferation and rooting cultivation. Results showed that: the optimum medium for breaking dormancy to germination was MS+2.0 mg/L 6-BA+1.0 mg/L NAA; the optimum medium for callus induction was MS+3.0mg/L 6-BA+0.3mg/L NAA; the medium with optimum shoot proliferation effect was MS+1.0mg/L 6-BA+0.3mg/L NAA; MS+0.6mg/L NAA was the best condition for root growth. ©, 2014, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.


Kong Q.,Hunan Vegetable Research Institute | Kong Q.,Huazhong Agricultural University | Zhang G.,Hunan Vegetable Research Institute | Zhang G.,Central South University | And 4 more authors.
American Journal of Botany | Year: 2012

Premise of the study: The redundancies in expressed sequence tags (ESTs) in the National Center for Biotechnology Information sequence database were used to identify and develop polymorphic simple sequence repeat (SSR) markers for pepper (Capsicum annuum). Methods and Results: Sixty-eight polymorphic SSR loci were identified in the contigs (containing redundant ESTs) generated by assembling 118 060 pepper ESTs from the public sequence database. Thirty-three SSR markers exhibited polymorphism among 31 pepper varieties, with alleles per SSR marker ranging from two to six. The mean observed and expected heterozygosity were 0.28 and 0.39, respectively. There were 18 SSR markers with a motif repeat number of less than five, accounting for 55% of the total. Conclusions: We demonstrated the value of mining the redundant sequences in public sequence databases for the development of polymorphic SSR markers, which can be used for marker-assisted breeding in pepper. © 2012 Botanical Society of America.

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