Vegetable Institution of Hunan Academy of Agricultural Science

Changsha, China

Vegetable Institution of Hunan Academy of Agricultural Science

Changsha, China
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Ouli-Jun,Vegetable Institution of Hunan Academy of Agricultural Science | Chao-Hui Z.,Hunan Academy of Agricultural Science | Zhou-Bin L.,Vegetable Institution of Hunan Academy of Agricultural Science | Ge W.,Vegetable Institution of Hunan Academy of Agricultural Science | And 3 more authors.
Pakistan Journal of Botany | Year: 2017

In this study, we studied the mitigation effects of exogenous Methyl jasmonate (MeJA) on waterlogging-induced damages to Xinyou No.5 wrinkled skin pepper cultivar by spraying MeJA on leave’s surface at different waterlogging periods and investigated its underlying mechanisms. The results showed that administration of MeJA increased antioxidant enzymes’ activities, proline and soluble sugar contents and alcohol dehydrogenase (ADH) activity, reduced relative conductivity, malondialdehyde (MDA) and hydroxyl free radical (·OH) accumulation, lactate dehydrogenase (LDH) activity and lactic acid and acetaldehyde accumulation, and maintained high root malate dehydrogenase (MDH) and succinate dehydrogenase (SDH) activities and certain aerobic respiratory metabolism. The study also found that there were significant differences among exogenous MeJA treatments at different waterlogging periods. Peppers treated with exogenous MeJA 1 day and 2 days prior to waterlogging had optimal agronomic traits, higher chlorophyll content, enzymatic activities and osmolytic substances, as well as lower relative conductivity, MDA and ·OH accumulation. Overall, the results suggest that MeJA mitigates waterlogging-induced damages to pepper by adjusting osmolytic substances contents, antioxidant enzymatic activities and root respiration and metabolism and achieves better alleviation effects by spraying prior to waterlogging. © 2017, Pakistan Botanical Society. All rights reserved.


Lijun O.,University of South China | Lijun O.,Huaihua University | Lijun O.,Vegetable Institution of Hunan Academy of Agricultural Science | Xuexiao Z.,University of South China | Xuexiao Z.,Vegetable Institution of Hunan Academy of Agricultural Science
African Journal of Biotechnology | Year: 2012

This paper studied the genetic diversity of five cultivated pepper species using inter simple sequence repeat (ISSR) analysis. The amplicons of 13 out of 15 designed primers were stable polymorphic and therefore were used as genetic biomarkers. 135 total clear bands were obtained, of which 102 were polymorphic bands with an average polymorphism rate of 75%. The cultivated species Capsicum annuum L. had less specific genotype bands compared with two wildtype species; its Nei's gene diversity index, Shannon's diversity index and genetic differentiation index were 0.21, 0.33 and 0.8306, respectively. The genetic variations of these five species were mainly interspecifically rather than intraspecifically. Cluster analysis showed that they belonged to different branches: C. annuum L., Capsicum chinense Jacquin. and Capsicum frutescens L. were in one cluster, whereas Capsicum baccatum L. and Capsicum pubescens Ruiz and Pavon were in another cluster. C. annuum L. had the least genetic polymorphism. Our results suggested that ISSR markers are valid tags in the studies on pepper genetic diversity and that cultivated species identification and gene exchange between cultivated and wild species should be strengthened in pepper breeding to improve heterosis. © 2012 Academic Journals.


Ou L.J.,University of South China | Ou L.J.,Huaihua University | Zou X.X.,University of South China | Zou X.X.,Vegetable Institution of Hunan Academy of Agricultural Science
Photosynthetica | Year: 2012

The aim of the study was to investigate the genetic distances and their relationships among pepper species using photosynthetic features under different stresses and genetic variability. The photosynthetic features under drought, waterlogging and low-temperature stresses, rDNA internal transcribed spacer (ITS) sequences of nuclear genome and trnH-psbA sequence of chloroplast genome of 25 varieties from 5 pepper species Capsicum annuum L. (CA), Capsicum baccatum L. (CB), Capsicum chinense Jacquin. (CC), Capsicum frutescens L. (CF) and Capsicum pubescens Ruiz & Pavon (CP) were analyzed and used to construct the dendrograms. The results showed the photosynthetic rate of different pepper species could be greatly but differentially decreased by stresses. For example, CB and CF had the smallest and the highest decrease to drought, CC had the highest decrease to waterlogging, and CP had the smallest decrease to low temperature. The ITS sequences of 25 pepper varieties are 591-619 bp in length and have GC% between 51.1% and 64.5%. Their trnH-psbA sequences are 537-558 bp in length and have GC% between 27.2% and 28.5%. The cluster analysis of the five pepper species based on the changes in P N under stresses is similar to that based on genetic variability, that is, CP clusters with CB, and CC clusters with CA after first clusters with CF. In addition, the clustering methods based on the photosynthetic stress responses and genetic variability are unable to completely distinguish pepper varieties within the same species. The results indicate that similarly to genetic variability, changes in P N under stresses (specifically the stress corresponding to the climate of plant's original habitat) could be used to identify genetic distance of pepper species. © 2012 Springer Science+Business Media B.V.


Lijun O.,Vegetable Institution of Hunan Academy of Agricultural Science | Lijun O.,Huaihua University | Zhuqing Z.,Vegetable Institution of Hunan Academy of Agricultural Science | Xiongze D.,Vegetable Institution of Hunan Academy of Agricultural Science | Xuexiao Z.,Vegetable Institution of Hunan Academy of Agricultural Science
Acta Physiologiae Plantarum | Year: 2012

This study compared the sequence variations and expressions of 12 chloroplastic and 8 mitochondrial genes in three pepper (Capsicum annuum L.) cytoplasmic male sterile (CMS) lines, their maintainers and two control cultivars. The results showed that the three CMS lines were highly similar in chloroplastic and mitochondrial fragment sequences, with average similarities of 96. 81 and 98. 73 %, respectively, and their chloroplastic trnH-psbA intergenic spacer, photosystem II 47 kDa protein (psbB) genes, mitochondrial apocytochrome b (cob) and RNAD fragments have 1, 9, 8 and 7 distinct sites from the maintainer lines, respectively, and could be used as informative sites to distinguish CMS lines from the maintainer lines. Meanwhile, the expressions of mitochondrial cob, RNAD and pvs in the reproductive organs (flowers) of CMS lines are different from those of the maintainer lines, but their expressions in the vegetative organs (roots and leaves) are similar. The results indicate that cytoplasmic DNA polymorphisms are rare in CMS lines, and mitochondrial cob, RNAD and pvs genes are closely related to pollen abortion. © 2012 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.


Ou L.-J.,Vegetable Institution of Hunan Academy of Agricultural Science | Ou L.-J.,Huaihua University | Zhang Z.-Q.,Vegetable Institution of Hunan Academy of Agricultural Science | Dai X.-Z.,Vegetable Institution of Hunan Academy of Agricultural Science | Zou X.-X.,Vegetable Institution of Hunan Academy of Agricultural Science
PLoS ONE | Year: 2013

Huai Zi (HZ) is a new purple mutant of green pepper (PI 631133) that is obtained from the United States Department of Agriculture. The net photosynthetic rate (PN), chlorophyll fluorescence parameters, antioxidant substances, antioxidant enzymes, photosystem 1 (PS1) and PS2 activities were studied through methyl viologen (MV) treatment. The results showed that the PN, actual photochemical efficiency of PS2 (ΦPS2), photochemical quenching coefficient (qP), PS1 and PS2 activities in HZ were lower than those in green pepper. HZ had a stronger ability to eliminate reactive oxygen species(O2 •-) and accumulated less malondialdehyde (MDA) (a membrane lipid peroxidation product) than did green pepper, and had a higher content of antioxidants and antioxidant enzyme activity. This suggests that the lower light energy absorption and higher thermal dissipation and antioxidant activity of HZ contributed to a more stable PS2 photosynthetic capacity, which resulted in photooxidation tolerance. Hence, our study strongly suggests that pepper hybrids can achieve a modest ratio of chlorophyll and anthocyanin content, high PN and resistance to photooxidation, improving yield and resistance to adverse environments. © 2013 Ou et al.


Ou L.,Huaihua University | Ou L.,Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources | Ou L.,Vegetable Institution of Hunan Academy of Agricultural Science | Ou L.,Key Laboratory of Hunan Higher Education for Hunan western Medicinal Plant and Ethnobotany | And 2 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2012

Increased human activities have had a significant impact on the global climate. Abnormal changes, such as drought stress caused by long-term un-rain, can affect plant growth and development. Pepper (Solanaceae), originally planted in tropical areas of South America, not only has medicinal value it has the highest vitamin C content of all vegetables. The International Board for Plant Cenetic Resources (IBPCR) has classified pepper species into five species; Capsicum annuum L. (CA), Capsicum baccatum L. (CB), Capsicum chínense Jacquin. (CC), Capsicum frutescens L. (CF), Capsicum pubescens Ruiz and Pavon (CP). CA is the most differentiated, widely cultivated species being the focus of pepper breeding worldwide. Screening of resistant germplasms is one of the most important measures against abnormal climate changes. Pot experiments were employed to determine the photosynthetic response and associated physiological characters, such as superoxide dismutase (SOD) and glutathione reductase (CR) activities, root activity and adventitious root number, of five domesticated and one wild pepper species (C baccatum var. baccatum (CBY)) to drought stress. The photosynthetic pigment content decreased the net photosynthetic rate (PN) of all tested peppers continued to decline on the first day of regular irrigation, then started to increase on the second day, and almost recovered to normal levels by the seventh day. Non-photochemical quenching (q N), water use efficiency (WUE), and root activity had increased dramatically, the SOD and CR activities of all the pepper species first increased, being highest on day 4, and subsequently decreased. The number of adventitious roots slightly increased in all peppers, suggesting that pepper has a stronger resistance to drought stress. The results also show that changes in the above parameters vary in different species. CB had smaller decreases in photosynthetic pigment content, PN and stomatal conductance (gs,), and greater increases in qN, WUE, SOD and CR activities and root activity, indicating that CB has the strongest resistance to drought stress. CC and CF showed bigger decreases in photosynthetic pigment content, PN and gs with smaller increases in qN, WUE, SOD, and CR activities and root activity, indicating they have weaker drought stress resistance. In addition, the wild pepper, C. baccatum var. baccatum (CBY), had the smallest decrease in photosynthetic pigment content, PN and gs with the greatest increases in qN, WUE, SOD, and CR activities, and root activity. This indicates that CBY has better resistance to adverse environments than all tested species, which is perhaps caused by the loss of stress resistance genes during the process of domestication. Our study found that, of the species tested, the ability to resist drought stress is strongest in wild pepper CBY. Of the five species tested, CB had the highest photosynthetic rate and strongest drought resistance capability. In conclusion, we suggest that crossbreeding of domestic and wild pepper species be considered for commercial breeding. Such a measure will not only expand the genetic pool, increasing genetic distance and eventually increasing production; it will also to introduce elite stress tolerance genes improving each species' ability to resist adverse environments.


Lijun O.,Vegetable Institution of Hunan Academy of Agricultural Science | Lijun O.,Huaihua University | Zhuqing Z.,Vegetable Institution of Hunan Academy of Agricultural Science | Xiongze D.,Vegetable Institution of Hunan Academy of Agricultural Science | Xuexiao Z.,Vegetable Institution of Hunan Academy of Agricultural Science
Plant Systematics and Evolution | Year: 2012

To illustrate the effects of domestication on wild pepper, we compared the seed micromorphology and germination characteristics of two wild pepper strains and their corresponding cultivars. The results showed that compared to their corresponding cultivars, wild pepper seeds are significantly smaller, have more groove and ridges on the surface, higher water imbibition rate, higher germination rate and higher α-amylase activity, and digest starch granules faster. The results indicate that the smaller wild seeds are easier to spread; more grooves and ridges on the surface and higher hilum ratio benefit their water imbibition, and rapid decomposition of starch granules and higher α-amylase activity are in favor to germination. By contrast, increased seed size will lead to a reduced germination rate and decreased adaptive ability to water environmental change. Thus, seed size is the result of natural and artificial selection, and whether larger seeds of various pepper cultivars are meaningful in evolution is debatable. © 2012 Springer-Verlag.


Ou L.J.,Vegetable Institution of Hunan Academy of Agricultural Science | Ou L.J.,Huaihua University | Dai X.Z.,Vegetable Institution of Hunan Academy of Agricultural Science | Zhang Z.Q.,Vegetable Institution of Hunan Academy of Agricultural Science | Zou X.X.,Vegetable Institution of Hunan Academy of Agricultural Science
Photosynthetica | Year: 2011

One of the effective ways to address the effects of abnormal climate change on plant is to find germplasms that have better resistance to adverse environments. In this paper, we studied the responses of 5 pepper species Capsicum annuum L. (CA), C. baccatum L. (CB), C. chinense Jacquin. (CC), C. frutescens L. (CF) and C. pubescens Ruiz & Pavon (CP) as well as a wild pepper C. baccatum var. baccatum (CBY) to waterlogging stress. The results showed that warterlogging treatment greatly decreases photosynthetic pigment content, net photosynthetic rate (PN) and stomatal conductance (gs), and dramatically increases proline content and water-use efficiency (WUE) in all tested pepper, suggesting that pepper has weak resistance to waterlogging stress. The results also showed that changes of the above parameters vary in different species. CP had the smallest decreases in photosynthetic pigment content, PN, and gs and greatest increases in proline content and WUE. By contrast, CC had the greatest decreases in photosynthetic pigment content, PN, and gs and smallest increases in proline content and WUE, indicating that different species had different resistance to adverse environment and species CP and CC had the strongest and the weakest resistances, respectively. In addition, the study also demonstrated that wild pepper CBY had better resistance to adverse environment than all the tested species, indicating loss of the stress resistance genes during the process of domestication. Taking together, our study strongly suggests that pepper species should crossbreed with other species and wild pepper to expand genetic diversity, enlarge genetic distance, promote production, and improve the resistance to adverse environments. © 2011 Springer Science+Business Media B.V.


Ou L.J.,Vegetable Institution of Hunan Academy of Agricultural Science | Wei G.,Vegetable Institution of Hunan Academy of Agricultural Science | Zhang Z.Q.,Vegetable Institution of Hunan Academy of Agricultural Science | Dai X.Z.,Vegetable Institution of Hunan Academy of Agricultural Science | Zou X.X.,Vegetable Institution of Hunan Academy of Agricultural Science
Photosynthetica | Year: 2014

Pepper is a thermophilous and heliophilic vegetable. In China, pepper is grown in greenhouse during winter and spring under lower temperature and irradiation. In this study, we investigated the effects of low temperature and low irradiance (LTLI) on the physiological characteristics and the expression of related genes in five pepper species, Capsicum annuum L. (CA), C. baccatum L. (CB), C. chinense Jacquin (CC), C. frutescens L. (CF), and C. pubescens Ruiz & Pavon (CP) in order to screen for greenhouse species that is resistant to such adverse conditions. We observed significant reductions not only in photosynthetic pigments and stomatal conductance but also in proline, total soluble sugar, enzyme activity, and root activity; disordered arrangements of leaf palisade and spongy tissues; and first rising and then falling expression of C-repeat binding factor (CBF3) and cold-regulated genes (CORc410). These results indicate that pepper is not resistant to LTLI. We also found that CP showed significantly higher photosynthetic activity, more proline and total soluble sugar, higher enzyme activity, higher root activity, higher CBF3 and CORc410 expression levels, more tightly packed leaf palisade and spongy tissues, and thicker bundle sheath than the other four species did under LTLI, while CF exhibited the lowest values for these indicators. It demonstrated significant differences in the ability to resist to LTLI among different species, with CP showing the strongest resistance, followed by CB. Therefore, we recommend the introduction of CP and CB to greenhouse cultivation to further screen for low temperature and low light-resistant pepper varieties to increase pepper production by strengthening intervariety hybridization. © 2015, The Institute of Experimental Botany.


Yang B.Z.,Central South University | Yang B.Z.,Vegetable Institution of Hunan Academy of Agricultural Science | Liu Z.B.,Central South University | Liu Z.B.,Vegetable Institution of Hunan Academy of Agricultural Science | And 11 more authors.
Photosynthetica | Year: 2016

Ca2+ has been considered as an necessary ion for alleviation of stress-induced damages in plants. We investigated effects of exogenous Ca2+ on waterlogging-induced damage to pepper and its underlying mechanisms. Pepper seedlings under stress were treated by spraying of 10 mM CaCl2. Applying exogenous Ca2+ increased the biomass of pepper leaves and roots, improved photosynthetic characteristics, membrane permeability, root activity, osmotic substance contents, antioxidant enzyme and alcohol dehydrogenase activities, while it reduced lactate dehydrogenase activity. It maintained hydroxyl free radical contents and activities of malate dehydrogenase and succinate dehydrogenase relatively high. Our results suggested that applying exogenous Ca2+ could regulate osmotic substance contents, antioxidant system activity, root respiration, and metabolism, and subsequently alleviate waterlogging-induced damages to pepper plants. © 2016 The Institute of Experimental Botany

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