Research Group Vascular Genomics

Bad Nauheim, Germany

Research Group Vascular Genomics

Bad Nauheim, Germany
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Kubin T.,Kerckhoff Clinic | Kubin T.,Research Group Vascular Genomics | Cetinkaya A.,Kerckhoff Clinic | Schonburg M.,Kerckhoff Clinic | And 3 more authors.
Cytokine | Year: 2017

PDGF-AB and FGF-2 (GFs) induce smooth muscle cell (SMC) proliferation which is indispensible for arteriogenesis. While there is common agreement that GFs stimulate SMC proliferation through phosphorylation (P-) of MEK1/2 at Ser218/222, we previously demonstrated that the MEK inhibitors PD98059 and UO126 did not inhibit P-Ser218/222 as originally proposed but caused strong hyperphosphorylation. Here, we demonstrate that GFs increased phosphorylation of MEK1 at Thr292 while UO126 and PD98059 blocked this phosphorylation. This was again surprising since phosphorylation of Thr292 is regarded as a negative feedback loop. Our findings suggest that inhibition of Thr292 phosphorylation in combination with hyperphosphorylation of Ser218/222 serves as an “off” switch of SMC proliferation and potentially of arteriogenesis. © 2017 Elsevier Ltd

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