Vasantdada Sugar Institute

Pune, India

Vasantdada Sugar Institute

Pune, India
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Mahamuni S.V.,Shardabai Pawar Mahila College | Shinde N.S.,Shardabai Pawar Mahila College | Wani P.V.,Mahatma Phule Agricultural University | Patil A.S.,Vasantdada Sugar Institute
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017

Objective: The purpose of the present investigation was to assess antifungal characterization of Burkholderia gladioli strain VIMP03 (JQ867372), an isolate from sugar beet rhizosphere. Methods: Antifungal characterization was carried out by biochemical, dual culture, and agar well diffusion methods against Ceratocystis paradoxa, a soilborne pathogen of sugarcane and other crops. Culture filtrate and ethyl acetate extract obtained from culture supernatant were analyzed by high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses, respectively. Results: The culture filtrate and ethyl acetate extract exhibited effective antifungal activity. Organic acid profile of the culture filtrate was determined. Acetic acid was mainly produced by the culture under study. The GC-MS analysis of ethyl acetate extract documented the presence of compounds including tetratetracontane, 9-nonadecene, erucic acid, and other hydrocarbon derivatives. Conclusion: The GC-MS, HPLC, and biochemical profiles of B. gladioli strain VIMP03 (JQ867372) revealed its agro clinical-antifungal potential. © 2017 The Authors.

Rajesh G.,Swami Ramanand Teerth Marathwada University | Harshala S.,Swami Ramanand Teerth Marathwada University | Dhananjay G.,Swami Ramanand Teerth Marathwada University | Jadhav A.,Swami Ramanand Teerth Marathwada University | Vikram G.,Vasantdada Sugar Institute
European Journal of Pharmaceutical Sciences | Year: 2010

Angiogenesis is a key process needed for the growth and survival of solid tumors. Anti-angiogenesis may arrest the tumor growth and keep check on cancer metastasis. Developing antiangiogenic agents have remained a significant hope in the mainstream of anticancer research. The free radical implications in the initiation of cancers are well established. In the present studies, simple flavone and flavones with hydroxyl substitution in 'A' and 'C' ring at 3, 5, 6, and 7 were studied for antiangiogenic activities using chorioallantoic membrane (CAM) model and kinetics of DPPH (2,2-diphenyl-1-picryl hydrazine) and superoxide anion radical (SOR) scavenging activities. The docking of selected flavones with specific angiogenic targets such as vascular endothelial growth factor (VEGF), hypoxia inducible factor (HIF-1α) and vascular endothelial growth factor receptor-2 (VEGFR2) from human origin was carried out to focus the possible underlying mechanism of anti-angiogenesis. The result of the present studies shows that the 3-hydroxy substitution of the flavone was found to be the most promising lead for antiangiogenic activity in CAM model. The same was true for DPPH reduction with greater velocity as compared to other hydroxyl substitutions. However the 7- and 6-hydroxy substitution were observed to be more favourable for SOR scavenging activities as compared to other hydroxyl substitutions. The docking experiments shows that the VEGFR2 seems to be a structurally compatible target for tight binding of the flavones especially with 3-hydroxy substitution (-9.78 kcal/mole) as compared to VEGF (-8.47 kcal/mole) and HIF-1α (-8.99 kcal/mole). The quantum chemical descriptors of the test flavones related to free radical scavenging and other biological activities were calculated using computational tools. The data is discussed in the light of structure-activity relationship. © 2009 Elsevier B.V. All rights reserved.

Prabu G.,Vasantdada Sugar Institute | Prabu G.,Shivaji University | Prabu G.,Karpagam University | Prasad D.T.,Vasantdada Sugar Institute | Prasad D.T.,University of Agricultural Sciences, Bangalore
Plant Cell Reports | Year: 2012

The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) flanking the 5′ ScMYBAS1 coding region from the sugarcane genome. A series of PScMYBAS1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -843, -1,033) was fused to the uidA reporter gene (GUS) and each deletion construct was analyzed by Agrobacterium-mediated transient transformation in tobacco leaves subjected to dehydration, salinity, cold, wounding, gibberellic acid (GA), salicylic acid (SA), and methyl jasmonic acid (MeJA). Deletion analysis of the promoter, PScMYBAS1, suggested that the 303-bp promoter region was required for basal expression. Promoter fragments, 777 bp or longer showed ~twofold to ~fourfold increased induction of GUS in response to abiotic stress (dehydration, salt, cold, wounding) and hormone (SA, MeJA) treatments. These findings further our understanding of the regulation of ScMYBAS1 expression and provide a new stress-inducible promoter system in transgenic plants. © 2011 Springer-Verlag.

Prabu G.,Vasantdada Sugar Institute | Prabu G.,Shivaji University | Prabu G.,Karpagam University | Kawar P.G.,Vasantdada Sugar Institute | And 3 more authors.
Plant Molecular Biology Reporter | Year: 2011

Sugarcane represents an important renewable source among biofuel crops with high capability to assimilate carbon among the C4 plants. Limited availability of freshwater renders this crop uneconomical, warranting the necessity for the development of varieties with higher water use efficiency and tolerant to water deficiency stress. Sugarcane variety cv. Co740 was subjected to varied levels of water deficiency stress to isolate transcripts differentially expressed to the imposed stress. The leaf relative water content was used as a measure to estimate the stress response. PCR-based cDNA suppression subtractive hybridization technique was applied to construct forward subtracted library for differentially expressed genes under stress. Dot blot-selected 158 clones showing elevated response were sequenced, of which 62% resembled similarity with known functional genes, 12% with hypothetical proteins of plant origin, while 26% represented new unknown sequences. Annotation of these differentially expressed sequence tags (ESTs) in the moderately water deficit stress-tolerant cultivar predicted that most of them encoded proteins involved in cellular organization, protein metabolism, signal transduction, and transcription. Further, semi-quantitative reverse transcriptase PCR carried out for five genes projected the involvement of these ESTs in stress alleviation/tolerance. Results from this study may help in targeting useful genes for improving drought tolerance in sugarcane and other grasses. © 2010 Springer-Verlag.

Dongare M.L.,S M Joshi College | Buchade P.B.,Abasaheb Garware College | Awatade M.N.,Vasantdada Sugar Institute | Shaligram A.D.,University of Pune
Optik | Year: 2014

Optical techniques developed for sensing and analysis purposes have been used in various fields. An attempt has been made to design optimization of refractormetric based method for the measurement of Brix. Mathematical modeling and simulation of the proposed system has been performed. From the study it is seen that mathematical model may help for getting the better performance to developed experimental model. Optimization of various constructional parameters including selection and location of source, prism and detector, position of source, angular position and height of source from prism plane, divergent angle of source, refractive index of prism, size of prism, the location of detector to pickup the optimum reflected light, refractive index of sample, critical angle, choice of suitable prism. The various possibilities of mounting position of source, detector, prism are studied. The mathematical model is developed considering the above mentioned parameters. © 2013 Elsevier GmbH.

Vohra M.,Vasantdada Sugar Institute | Manwar J.,Maharashtra Institute of Pharmacy | Manmode R.,University of Massachusetts Lowell | Padgilwar S.,Maharashtra Institute of Pharmacy | Patil S.,Vasantdada Sugar Institute
Journal of Environmental Chemical Engineering | Year: 2014

Fossil fuels such as oil, coal and natural gases have become the prime sources of energy in the current era. However, it is anticipated that these sources will deplete within the next 40-50 years. The expected environmental damages like global warming, acid rain and urban smog have tempted us to reduce the carbon emissions by 80% (v/v) and shift toward utilizing a variety of renewable energy resources such as solar, wind, biofuel, etc. that are less environmentally harmful in a sustainable way. Ethanol is one of the most promising alternative biofuel. Although the energy equivalent of ethanol is 68% lower than that of petroleum fuel, the combustion of ethanol is cleaner (because it contains oxygen) and thus it recognize as a potential biofuel alternative to gasoline. Ethanol has been frequently used for the blended gasoline in the concentration range 10-85% (v/v). More recently, ethanol is identified as a fuel for the direct ethanol fuel cells (DEFC) and biofuel cells. Sugarcane and corn feedstock, are the main source of ethanol. Nevertheless, it is barely sufficient to meet the current demand. Lignocellulosic biomass is an alternative source but its availability is poorly documented. This review discusses the current status of ethanol production from different feedstocks and the state of technologies involved in ethanol production from such different feedstock. © 2013 Elsevier Ltd.

Pagariya M.C.,Vasantdada Sugar Institute | Pagariya M.C.,Shivaji University | Devarumath R.M.,Vasantdada Sugar Institute | Devarumath R.M.,Shivaji University | Kawar P.G.,Vasantdada Sugar Institute
Plant Science | Year: 2012

Sugarcane productivity is worldwide subjected to increasing environmental constraints, predominantly to drought and salinity owing to their high magnitude of impact and wide distribution. The present study provides insights into the knowledge of sugarcane responses at germination, tillering and respective recovery stages to high salinity at physio-biochemical and molecular level. Our results indicated that there was a negative relationship between catalase, and peroxidase activity with lipid peroxidation and SOD activity. Increase in MDA and SOD levels at the earlier stages of stress and later increase in CAT and POD levels on prolonged stress was evidenced. Thus they can be used as indicators of stress for sugarcane plants facing unfavourable environmental conditions. At molecular level, we have identified 137 salinity tolerant candidate cDNAs from sugarcane by cDNA-SSH, representing 20% of which are novel sugarcane genes. These unique sequences, never reported elsewhere to be stress related and might provide further understanding on perception, response and adaptations mechanisms of the non-model plant like sugarcane to salinity stress and will be potential candidates for manipulating salt tolerant trait. © 2011 Elsevier Ireland Ltd.

Devarumath R.M.,Vasantdada Sugar Institute | Kalwade S.B.,Vasantdada Sugar Institute | Kawar P.G.,Vasantdada Sugar Institute | Sushir K.V.,Vasantdada Sugar Institute
Sugar Tech | Year: 2012

Molecular diversity within and between Saccharum species clones and elite commercial hybrid varieties was studied using intersimple sequence repeat (ISSR) and simple sequence repeat (SSR) markers. The present study was performed to characterize 81 sugarcane genotypes. A total of 13 ISSR primers used and produced 65 amplified fragments, of which 63 (96.5 %) were polymorphic. The Polymorphic Information Content (PIC) value ranged from 0. 11 (UBC824) to 0.45 (UBC825) primers with an average value of 0.28. The primer UBC 817 and UBC 825 exhibited highest resolving power (Rp) value 3.8 among thirteen primers. Genetic similarity (GS) by Jaccard's similarity coefficient ranged from 0.23 to 0.95 with a mean of 0.59. Of the 79 alleles amplified by 28 SSRs primers showed 76 alleles, which were found to be polymorphic (96.2 %). The PIC value ranged from 0.06 (VSICRAD4) to 0.55 (VSICRAD26) primers with an average value of 0.17. The primer VSICRAD23 exhibited highest resolving power (Rp) value 4. 3 among 28 primers. The GS by Jaccard's similarity coefficient ranged from 0.11 to 0.91 with a mean of 0.51. Dendrograms constructed using the UPGMA cluster analysis revealed low level of correlation between genetic similarities based on pedigree and DNA profiles. The ISSR and SSR amplification proved to be valuable method for assessing genetic diversity among sugarcane complex and their related wild varieties and for identification of the cultivars. © 2012 Society for Sugar Research & Promotion.

Kalwade S.B.,Vasantdada Sugar Institute | Kalwade S.B.,Shivaji University | Devarumath R.M.,Vasantdada Sugar Institute | Devarumath R.M.,Shivaji University
Applied Biochemistry and Biotechnology | Year: 2014

Sugarcane (Saccharum spp.) is a dynamic C4 polyploidy grass used as a major source of sucrose and an alternative for ethanol, food, and energy. Despite growing scientific interest, various sucrose metabolism regulatory aspects have been limited. Biochemical and gene expression studies were conducted on developmental stages, 240-420 days of planting (DAP) in mature leaves of three high and three low sucrose sugarcane cultivars. Sucrose synthase (SS) and sucrose phosphate synthase (SPS) activities were found to be remarkably higher at 240-360 DAP but decrease at 420 DAP. Twofold increases of SS activity was estimated at 240-360 DAP while SPS activity trend was found to be lower than the SS activity. In comparing SS and SPS activities with the brix of respective DAP, results show that these activities are significant and positively correlated with 'r' values of 0.69 and 0.68 for SS and SPS, respectively. However, the soluble acid invertase (SAI) and neutral invertase (NI) activities were found to decrease significantly with the maturity of cultivars, negatively correlating with brix at 'r' values 0.83 and 0.89 for SAI and NI, respectively. The antioxidant enzyme activity was modulated similar to the invertases activity. Of the six genes, ESAS 11 and 23 associated with sucrose accumulation and ESTS 34 and 41 associated with sugar transport in sugarcane were differentially expressed among the selected high and low sugarcane cultivars. Hence, these findings reinforce the selection of diverse sugarcane cultivars for gene expression studies targeting to quantitative traits and candidate marker determination. © 2013 Springer Science+Business Media.

Sawant R.A.,Vasantdada Sugar Institute | Tawar P.N.,Vasantdada Sugar Institute
Sugar Tech | Year: 2011

Sodium hypochlorite has been successfully utilized as media sterilant (at total active chlorine concentration 0. 002% in the medium) in production of sugarcane plantlets by applying apical meristem culture technique. The process has been scaled up to produce 2. 5 million sugarcane plantlets per year. Use of sodium hypochlorite in place of autoclaving has resulted in reducing considerable amount of electricity cost and ultimately reducing the cost of production of sugarcane plantlets. © 2011 Society for Sugar Research & Promotion.

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