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Joshi P.S.,Vasantdada Patil Dental College and Hospital | Golgire S.,Tatyasaheb Kore Dental College and Hospital
Journal of Oral and Maxillofacial Pathology | Year: 2014

Context: The enzyme lactate dehydrogenase (LDH), which is found in almost all the cells of body tissues, can be separated into five fractions and the isoenzyme pattern is believed to vary according to the metabolic requirement of each tissue. LDH concentration in saliva, as an expression of cellular necrosis, could be considered to be a specific indicator for oral lesions that affect the integrity of the oral mucosa.Aim: The present study was designed to evaluate salivary LDH isoenzyme pattern in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC) and to correlate between LDH isoenzyme levels and histopathologic grading in selected cases of OL and OSCC.Materials and Methods: Clinically diagnosed 30 cases each of OL and OSCC were selected for the study and 30 healthy individuals of comparable age served as control. Unstimulated whole saliva was aseptically collected and was processed immediately for LDH isoenzymes measurement by agarose gel electrophoresis. Biopsy specimen obtained was processed and stained by hematoxylin and eosin. Sections of OL and OSCC cases were scrutinized histopathologically and appropriately graded for epithelial dysplasia and differentiation of carcinoma respectively.Statistical Analysis Used: Two sample t test for testing the significance of difference between two group means was used.Results and Conclusion: The present salivary analysis for LDH isoenzyme reveals an overall increased salivary LDH isoenzyme level in OL and OSCC cases and a significant correlation between levels of salivary LDH isoenzymes and histopathologic grades of dysplasia in OL and OSCC. Salivary analysis of LDH will definitely provide the clinician and/or the patient himself with an efficient, non invasive and friendly new tool for diagnosis and monitoring of oral precancer and cancer. Source

Malagan M.A.,Vishwanath Katti Institute of Dental science | Biswas P.P.,Royal Dental College | Muddaiah S.,Coorg Institute of Dental science | Reddy R.,Jodhpur Dental College General Hospital | And 4 more authors.
Journal of Clinical and Diagnostic Research | Year: 2014

Aim: To evaluate the rapidity and amount of separation of four different types of separators (i.e. Elastomeric separators, Dumbbell separators, Kesling springs and NEET springs) and also the percentage of loss of these separators. Materials and Methods: The separating effect of 4 different types of separators (i.e. Elastomeric separators, Dumbbell separators, Kesling springs and NEET springs) were assessed for 3 days, and the separated space between molars and premolars were noted separately for three consecutive days. The number and types of lost separators were recorded at the same time. The amount of separation between molars and 2nd premolar in each quadrant was measured separately with a leaf gauge (sensitivity 5/100mm) and noted on each day.Results: Dumbbell separators proved to be the fastest in producing separation and they produced consistently greater amount of separation on all three days. Conclusions: The Dumbbell separator would be ideal for situations where in rapid separation is needed, and also in cases where in the patient comes with the loss of separators. Source

Khambete N.,CSMSS Dental College and Hospital | Kumar R.,MGM Dental College and Hospital | Risbud M.,Vasantdada Patil Dental College and Hospital | Joshi A.,Al Ameen Medical College
Journal of Oral Biology and Craniofacial Research | Year: 2012

Objectives: The objective of the study was to determine whether digital panoramic radiography is a reliable method to detect calcified carotid artery atheromatous plaques (CCAAP) as compared to ultrasonography. Study design: Digital panoramic radiographs were obtained from 50 patients who also underwent carotid ultrasound examination. The images were interpreted by trained maxillofacial radiologist for the presence or absence of calcified atheromatous plaques. The extent of carotid calcification on carotid ultrasonography was determined by a trained sonologist which was considered as gold standard assessment. Results: Digital panoramic radiographs had sensitivity of 76% and specificity of 98.66% in determining calcified carotid artery atheromatous plaques. There was a high level of agreement between diagnoses, with a kappa value of 0.8. Conclusion: To conclude, digital panoramic radiographs had good sensitivity and high specificity in detecting calcified carotid artery atheromatous plaques. If properly trained, dentists can detect such plaques and can refer patients to physician for timely medical treatment. © 2012 Craniofacial Research Foundation. Source

Magadum D.,Bharti Vidyapeeth Dental College and Hospital | Sanadi A.,Vasantdada Patil Dental College and Hospital | Agrawal J.M.,Bharti Vidyapeeth Dental College and Hospital | Agrawal M.S.,Bharti Vidyapeeth Dental College and Hospital
BMJ Case Reports | Year: 2013

Lipoma is the commonest benign tumour occurring at any anatomical site where fat is present, but occurrence in the oral cavity is rare. Tongue which is totally devoid of fat cells is a rare site for lipoma. This is one such rare case of the universal tumour, presenting at the lateral margin of the tongue, for which complete tumour excision was done. Macroscopically the mass had a hard consistency and measured 3.0×2.0 cm. From microscopic examination, diagnosis of lipoma was made. Recurrence of tongue lipoma is rare. Source

Ankle M.R.,Vasantdada Patil Dental College and Hospital | Joshi P.S.,Vasantdada Patil Dental College and Hospital
Journal of Oral and Maxillofacial Pathology | Year: 2011

Context: Use of diluted dish washing solution (DWS) has been experimented successfully as a substitute for xylene to deparaffinize tissue sections during hematoxylin and eosin (H and E) staining. Aims: (1) Test the hypothesis that xylene- and methanol-free sections (XMF) deparaffinized with diluted DWS are better than or at par with conventional H and E sections. (2) To compare the efficacy of xylene-free sections with the conventional H and E sections. Settings and Design: Single blinded experimental study. Materials and Methods: Sixty paraffin blocks were considered. One section was stained with conventional H and E method (Group A) and the other with XMF H and E (Group B). Slides were scored for parameters; nuclear staining, cytoplasmic staining (adequate = score1, inadequate = score0), uniformity, clarity, crispness (present = score1, absent = score0). Score >/= 2 was inadequate for diagnosis and 3-5 was adequate for diagnosis. Statistical analysis used: Z test. Results: Adequate nuclear staining, 96.66% sections in group A and 98.33% in Group B (Z = 0.59, P>0.05); adequate cytoplasmic staining, 93.33% in group A and 83.33% in Group B (Z = 1.97, P<0.05); uniform staining, 70% in group A, 50% in group B (Z = 1.94, P<0.05), clarity present in 85% of group A, 88.33% of group B sections (Z = 0.27, P>0.05), crisp staining in 76.66% in group A and 83.33% in Group B (Z = 1.98, P<0.05), 88.33% Group A sections stained adequately for diagnosis as compared with 90% in Group B (Z = 0.17, P>0.05). Conclusion: Xylene- and methanol-free H and E staining is a better alternative to the conventional H and E staining procedure. Source

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