Vanguard AG

Berlin, Germany

Vanguard AG

Berlin, Germany
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Ehlbeck J.,Leibniz Institute for Plasma Science and Technology | Schnabel U.,Leibniz Institute for Plasma Science and Technology | Polak M.,Vanguard AG | Winter J.,Webeco GmbH and Co. KG | And 4 more authors.
Journal of Physics D: Applied Physics | Year: 2011

The aim of this paper is to provide a survey of plasma sources at atmospheric pressure used for microbicidal treatment. In order to consider the interdisciplinary character of this topic an introduction and definition of basic terms and procedures are given for plasma as well as for microbicidal issues. The list of plasma sources makes no claim to be complete, but to represent the main principles of plasma generation at atmospheric pressure and to give an example of their microbicidal efficiency. The interpretation of the microbicidal results remain difficult due to the non-standardized methods used by different authors and due to the fact that small variations in the setup can change the results dramatically. © 2011 IOP Publishing Ltd.

Polak M.,Vanguard AG | Winter J.,Webeco GmbH and Co. KG | Schnabel U.,Leibniz Institute for Plasma Science and Technology | Ehlbeck J.,Leibniz Institute for Plasma Science and Technology | Weltmann K.-D.,Leibniz Institute for Plasma Science and Technology
Plasma Processes and Polymers | Year: 2012

An innovative device capable of generating a cold atmospheric pressure plasma inside a 5 m long flexible tube with 2 mm inner diameter is presented. In order to analyze the inner-tube plasma, optical emission spectroscopy in the (vacuum) ultraviolet spectral range and Fourier transformed infrared spectroscopy were performed. By admixing small concentrations of nitrogen and oxygen to the standard argon gas flow rate of 1.5 slm, a drastically change in the plasma composition was observed. Additionally, it is possible to form a jet-like plasma at the end of the tube. The microbicidal efficacy of the inner-tube plasma and the jet-like plasma was shown for Bacillus atrophaeus spores. In this paper a setup is presented, to generate a DBD inside a long, flexible and thermo labile tube with the purpose to decontaminate the inner tube walls. The main application for these modified tubes is the implementation as biopsy channels in endoscopes, which are highly contaminated and difficult to sterilize. Besides decontamination of the inner tube it is also possible to ignite a jet-like discharge at the tube end. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Kohl Y.,Fraunhofer Institute for Biomedical Engineering | Oostingh G.J.,University of Salzburg | Sossalla A.,Fraunhofer Institute for Biomedical Engineering | Duschl A.,University of Salzburg | And 2 more authors.
Nanoscale Research Letters | Year: 2011

In most conventional in vitro toxicological assays, the response of a complete cell population is averaged, and therefore, single-cell responses are not detectable. Such averaging might result in misinterpretations when only individual cells within a population respond to a certain stimulus. Therefore, there is a need for non-invasive in vitro systems to verify the toxicity of nanoscale materials. In the present study, a micro-sized cell culture chamber with a silicon nitride membrane (0.16 mm 2) was produced for cell cultivation and the detection of specific cell responses. The biocompatibility of the microcavity chip (MCC) was verified by studying adipogenic and neuronal differentiation. Thereafter, the suitability of the MCC to study the effects of nanoparticles on a small cell population was determined by using a green fluorescence protein-based reporter cell line. Interleukin-8 promoter (pIL8) induction, a marker of an inflammatory response, was used to monitor immune activation. The validation of the MCC-based method was performed using well-characterized gold and silver nanoparticles. The sensitivity of the new method was verified comparing the quantified pIL8 activation via MCC-based and standard techniques. The results proved the biocompatibility and the sensitivity of the microculture chamber, as well as a high optical quality due to the properties of Si 3N 4. The MCC-based method is suited for threshold- and time-dependent analysis of nanoparticle-induced IL8 promoter activity. This novel system can give dynamic information at the level of adherent single cells of a small cell population and presents a new non-invasive in vitro test method to assess the toxicity of nanomaterials and other compounds. © 2011 Kohl et al.

Kohl Y.,Fraunhofer Institute for Biomedical Engineering | Gorjup E.,Fraunhofer Institute for Biomedical Engineering | Katsen-Globa A.,Fraunhofer Institute for Biomedical Engineering | Buchel C.,Goethe University Frankfurt | And 2 more authors.
Journal of Nanoparticle Research | Year: 2011

Gold nanoparticles are very attractive for biomedical products. However, there is a serious lack of information concerning the biological activity of nanosized gold in human tissue cells. An influence of nanoparticles on stem cells might lead to unforeseen consequences to organ and tissue functions as long as all cells arising from the initial stem cell might be subsequently damaged. Therefore the effect of negatively charged gold nanoparticles (9 and 95 nm), which are certified as reference material for preclinical biomedical research, on the adipogenic differentiation of human mesenchymal stem cells (hMSCs) is investigated here. Bone marrow hMSCs are chosen as differentiation model since bone marrow hMSCs are well characterized and their differentiation into the adipogenic lineage shows clear and easily detectable differentiation. In this study effects of gold nanoparticles on adipogenic differentiation are analyzed regarding fat storage and mitochondrial activity after different exposure times (4-21 days). Using time lapse microscopy the differentiation progress under chronically gold nanoparticle treatment is continuously investigated. In this preliminary study, chronically treatment of adipogenic differentiating hMSCs with gold nanoparticles resulted in a reduced number and size of lipid vacuoles and reduced mitochondrial activity depending on the applied concentration and the surface charge of the particles. © 2011 Springer Science+Business Media B.V.

Bracklo M.,Vanguard AG | Marczak U.,Vanguard AG | Chergui S.,Vanguard AG
European Cardiology | Year: 2012

This article reviews 15 years of experience with the reprocessing of cardiology medical devices in Germany, focusing on the requirements of the German regulatory framework, which, since 2002, are the same for the reprocessing of single-use and multiple-use devices. Since then, single-use device reprocessing has moved from a contentious procedure to the gold standard of product reprocessing in cardiology. In some cases, more than 70% of single-use devices are now being reprocessed in Germany. The accumulated evidence clearly demonstrates that reprocessing, when carried out by experienced and certified reprocessors with validated skills, is safe, sustainable and brings significant economic benefits. © Touch Briefings 2012.

Vanguard AG | Date: 2015-12-18

Cleaning system for cleaning at least one region of a medical device that can be brought in contact with a human or animal body. Means (14) for positioning the medical device in the cleaning system are present, such that the region that can be brought in contact with the human or animal body is positioned in a non-contact manner in the cleaning system.

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