Vhir Vall Dhebron Institute Of Recerca

Barcelona, Spain

Vhir Vall Dhebron Institute Of Recerca

Barcelona, Spain
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Giralt A.,University of Barcelona | Giralt A.,Institute dInvestigacions Biomediques August Pi i Sunyer IDIBAPS | Giralt A.,CIBER ISCIII | Sanchis D.,University of Lleida | And 13 more authors.
Experimental Neurology | Year: 2013

Endonuclease G (EndoG) has been largely related with a role in the modulation of a caspase-independent cell death pathway in many cellular systems. However, whether this protein plays a specific role in the brain remains to be elucidated. Here we have characterized the behavioral phenotype of EndoG-/- null mice and the expression of the nuclease among brain regions. EndoG-/- mice showed normal neurological function, learning, motor coordination and spontaneous behaviors. However, these animals displayed lower activity in a running wheel and, strikingly, they were consistently less anxious compared to EndoG+/+ mice in different tests for anxiety such as plus maze and dark-light test. We next evaluated the expression of EndoG in different brain regions of wild type mice and found that it was expressed in all over but specially enriched in the striatum. Further, subcellular biochemical experiments in neocortical samples from wild type mice revealed that EndoG is localized in pre-synaptic compartments but not in post-synaptic compartments. Altogether these findings suggest that EndoG could play a highly specific role in the regulation of anxiety by modulating synaptic components. © 2013 Elsevier Inc.


Rocas P.,Barcelona Institute for Research in Biomedicine | Fernandez Y.,Vhir Vall Dhebron Institute Of Recerca | Schwartz S.,Vhir Vall Dhebron Institute Of Recerca | Abasolo I.,Vhir Vall Dhebron Institute Of Recerca | And 4 more authors.
Journal of Materials Chemistry B | Year: 2015

Polyurethane-polyurea (PUUa) reactive prepolymers with adjusted hydrophobic and hydrophilic dangling chains to achieve multiwalled sub-30 nm nanoparticles are presented. The combination of an amphiphilic and a hydrophobic prepolymer at the oil-water interface creates a stratified shell by hydrophobic interactions. These novel nanostructures enhance the encapsulation stability of lipophilic compounds compared to monowalled nanostructures and facilitate the selective and ordered functionalization along the multiwalled shell with bioactive motifs. As proof of concept, PUUa nanoparticles have been engineered with disulfide bonds and an αvβ3 integrin-selective cyclic RGD peptide (cRGDfK) providing our system with glutathione (GSH) triggered controlled release and cell targeting specificity to U87 tumor cells. © The Royal Society of Chemistry 2015.


Ribas V.J.,Polytechnic University of Catalonia | Vellido A.,Polytechnic University of Catalonia | Ruiz-Rodriguez J.C.,Autonomous University of Barcelona | Ruiz-Rodriguez J.C.,Vhir Vall Dhebron Institute Of Recerca | Rello J.,Autonomous University of Barcelona
Expert Systems with Applications | Year: 2012

Sepsis is one of the main causes of death for non-coronary ICU (Intensive Care Unit) patients and has become the 10th most common cause of death in western societies. This is a transversal condition affecting immunocompromised patients, critically ill patients, post-surgery patients, patients with AIDS, and the elderly. In western countries, septic patients account for as much as 25% of ICU bed utilization and the pathology affects 1-2% of all hospitalizations. Its mortality rates range from 12.8% for sepsis to 45.7% for septic shock. The prediction of mortality caused by sepsis is, therefore, a relevant research challenge from a medical viewpoint. The clinical indicators currently in use for this type of prediction have been criticized for their poor prognostic significance. In this study, we redescribe sepsis indicators through latent model-based feature extraction, using factor analysis. These extracted indicators are then applied to the prediction of mortality caused by sepsis. The reported results show that the proposed method improves on the results obtained with the current standard mortality predictor, which is based on the APACHE II score. © 2011 Elsevier Ltd. All rights reserved.


Cabrera I.,Campus Universitari Of Bellaterra | Cabrera I.,CIBER ISCIII | Elizondo E.,Campus Universitari Of Bellaterra | Elizondo E.,CIBER ISCIII | And 33 more authors.
Nano Letters | Year: 2013

The integration of therapeutic biomolecules, such as proteins and peptides, in nanovesicles is a widely used strategy to improve their stability and efficacy. However, the translation of these promising nanotherapeutics to clinical tests is still challenged by the complexity involved in the preparation of functional nanovesicles and their reproducibility, scalability, and cost production. Here we introduce a simple one-step methodology based on the use of CO2-expanded solvents to prepare multifunctional nanovesicle- bioactive conjugates. We demonstrate high vesicle-to-vesicle homogeneity in terms of size and lamellarity, batch-to-batch consistency, and reproducibility upon scaling-up. Importantly, the procedure is readily amenable to the integration/encapsulation of multiple components into the nanovesicles in a single step and yields sufficient quantities for clinical research. The simplicity, reproducibility, and scalability render this one-step fabrication process ideal for the rapid and low-cost translation of nanomedicine candidates from the bench to the clinic. © 2013 American Chemical Society.

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