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Atessa, Italy

Di Tommaso D.,Valagro SpA
Agro Food Industry Hi-Tech | Year: 2012

Seaweeds growing in challenging environments have developed, since their presence in the Oceans, biochemical means to face biotic and abiotic stresses. The resulting molecules have a great potential as active ingredients for humans. In this article we underline the importance of 5 classes of substances in brown seaweeds, starting from their ecological role and getting to the functions they may have for human health, not forgetting that we are only at the beginning of the story, needing further testing and information to try to get the full picture of those truly ancient inhabitants of the Seven Seas.

Ferrante A.,University of Milan | Trivellini A.,University of Milan | Vernieri P.,University of Pisa | Piaggesi A.,Valagro SpA
Acta Horticulturae | Year: 2012

Biostimulants are generally able to improve mineral use efficiency, enhance plant tolerance to biotic and abiotic stresses and stimulate some physiological processes related to development. In our studies, Actiwave® was tested in floricultural crops such as Camellia japonica L. To speed up growth reducing the nursery. The experiments were performed on flowering camellia, using non-rooted cuttings treated with Actiwave® (Valagro Spa) and gibberillic acid (GA3). The Actiwave was applied providing 0.015 or 0.03 ml per cutting, while the GA3 was applied 1.25 or 2.5 mg per cutting. Treatments were repeated each month with eight applications in total. The biostimulant was distributed on the substrate with a volume of 100 ml. The GA3 instead was nebulized on the leaves and on the substrate of each cutting. Rooting was monitored during the whole experimental period. The measurements of fresh and dry weight were taken after 127 days. Results showed that both Actiwave® and GA3 stimulated the rooting compared with control. The two concentrations of Actiwave® had the same effect, with an average fresh root mass of 0.5 g. The treatment with GA3 was observed for a dose-effect response. The differences in dry weight were observed only on roots, and values were significantly higher in the treatments compared to the control.

Iriti M.,University of Milan | Vitalini S.,University of Milan | Di Tommaso G.,Valagro SpA | D'Amico S.,Valagro SpA | And 2 more authors.
Australian Journal of Grape and Wine Research | Year: 2011

Background and Aims: Chitosan, a chitin deacetylated derivative, is a biopolymer able to elicit the plant defence machinery against a variety of pathogens. In crop protection, it is successfully employed for its low toxicity, mainly against virus diseases. In this study, the efficacy of a new chitosan formulation (Kendal Cops ® (Kc)) was evaluated in grapevine against powdery mildew infection. Total polyphenol (TP) content and antiradical power of different grape tissues and of experimental wines produced from treated grapes were also assessed. Methods and Results: Field treatments with different Kc dilutions or with Kc alternated or mixed with fungicides, or with fungicides alone (penconazole and methyldinocap) were carried out in an experimental vineyard of the cv. Montepulciano d'Abruzzo. Kc was effective in controlling powdery mildew, particularly at the concentration of 0.1%, even under high disease pressure conditions. In this case, disease severity was 2.39% in treated grapes, compared with 87.5 and 0.92% in untreated and conventional fungicide-treated grapevines, respectively. TP content, measured by Folin-Ciocalteau colorimetric assay, was significantly higher in all Kc-treated grape tissues, compared with both untreated controls and fungicide-treated grapes. A similar trend was reported with regard to the antioxidant activity, assessed by 2,2-diphenyl-1-pycryl hydrazyl free radical scavenging test. Analysis of wines showed that both TPs and antiradical power were significantly higher in wines made from Kc-treated grapes compared with fungicide-treated ones. Conclusions: Kc not only protected grapevine from powdery mildew but also improved TP content and antioxidant activity in both grapes and wine. Significance of the Study: The possibility of using plant defence inducers in crop protection deserves particular attention because of their low environmental impact and of their ability to improve some quality traits of plant foodstuffs. Furthermore, in contrast to fungicides, they hardly select resistant pathogen strains and do not incur fitness costs for the plant. © 2011 Australian Society of Viticulture and Oenology Inc.

Santaniello A.,SantAnna School of Advanced Studies | Giorgi F.M.,Istituto di Genomica Applicata | Giorgi F.M.,University of Udine | Tommaso D.D.,Valagro SpA | And 3 more authors.
Acta Horticulturae | Year: 2012

DNA microarrays can be used to obtain a fingerprint of the transcriptional status of the plant or cell under a given condition and may be useful for characterising which genes respond, either by induction or repression, to novel stimuli or specific treatments. An in-depth bioinformatical analysis of all the data produced by microarrays can further highlight the metabolic or functional pathways most affected by the treatment. This approach has been used to investigate the effects induced by the treatment of different plant-derived raw materials, provided by Valagro SpA, on Arabidopsis seedlings. A clear example is represented by treatment with a raw plant-derived protein extract (VAL-P01). In this case the treatment induced genes related to ABA and osmotic stress treatment. We therefore demonstrated that VAL-P01 was able to mimic in planta the same pattern of responses linked to ABA treatment or osmotic stress, making the plant stronger against possible further stresses. Another plant extract, VAL-P02, was shown to be significantly altering the transcription of senescence genes, making it an ideal candidate adjuvant for the prolonged shelf-life of vegetal products.

Biasone A.,Valagro SpA | Cianci G.,Valagro SpA | Di Tommaso D.,Valagro SpA | Piaggesi A.,Valagro SpA | D'Alessandro N.,University Gdannunzio
Journal of Chromatography A | Year: 2013

Among the commercial ethylenediamine-N,N'-bis(2-hydroxy)phenylacetic acid/iron(III) derivatives, ethylenediamine-N,N'-bis(2-hydroxy-5-sulphophenylacetic) acid/iron(III) (EDDHSA/Fe) represents one of the promising chelates for the treatment of chlorotic plants. Industrial synthesis of EDDHSA/Fe leads to relevant amounts of o,o-EDDHSA condensation products (o,o-EDDHSAcps) and other secondary products that might have important relevance from the agronomic point of view. However, their chemical structures have remained unknown to date. Analysis of iron complexes by ion-pair reversed-phase chromatography, coupled with electrospray tandem mass spectrometry revealed the presence of the meso-o,o-EDDHSA/Fe, rac-o,o-EDDHSA/Fe, o,p-EDDHSA/Fe regioisomers, the hydroxyl derivative of o,o-EDDHSA/Fe, and the three main EDDHSA condensation products chelating the iron(III) (EDDHSAcps/nFe). However, the chromatographic peaks of EDDHSAcps/Fe are not well resolved due to the large numbers of stereoisomers and the poor efficiency of the ion-pair reversed-phase separation method. An alternative chromatographic method is based on porous graphitic carbon (PGC) separation after pre-column decomplexation of the chelates with trifluoracetic acid, which was developed to allow detection of EDDHSA stereo/regioisomers, EDDHSAcps, and low-molecular-weight by-products. This extensive PGC-HPLC-ESI-MS/MS investigation provides quantitative determination of meso-o,o-EDDHSA, rac-o,o-EDDHSA and o,p-EDDHSA, in addition to characterisation of EDDHSAcps and the low-molecular-weight by-products. PGC separation coupled to a triple quadrupole ESI-MS detector allowed characterisation of free ligands using collision-induced dissociation experiments in positive and negative ionisation mode, providing comparative evaluation of EDDHSAcps in three commercial samples. For detection, the PGC-HPLC-ESI-MS/MS is the best method according to the limit of quantification and limit of detection (picomolar and sub-picomolar detection, respectively) for determination of meso-EDDHSA and rac-o,o-EDDHSA. Synthesis, purification and quantification of o,o-EDDHSA and o,p-EDDHSA by 1H-nuclear magnetic resonance are also reported. © 2013 Elsevier B.V.

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