Wang X.,University of Texas Southwestern Medical Center |
Dong Y.,University of Texas Southwestern Medical Center |
Jiwani A.J.,University of Texas Southwestern Medical Center |
Jiwani A.J.,North Texas VA Healthcare System |
And 8 more authors.
Proteome Science | Year: 2011
An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways. © 2011 Wang et al; licensee BioMed Central Ltd.