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Kozlova E.,Moscow State University | Chernysh A.,Moscow State University | Moroz V.,Va Negovsky Scientific Research Institute Of General Reanimatology | Sergunova V.,Va Negovsky Scientific Research Institute Of General Reanimatology | And 2 more authors.
Experimental Cell Research | Year: 2015

Packed red blood cells (PRBC) are used for blood transfusion. PRBC were stored for 30 days under 4. °s{cyrillic} in hermetic blood bags with CPD anticoagulant-preservative solution. Hematocrit was 50-55%. The distortions of PRBC membranes nanostructure and cells morphology during storage were studied by atomic force microscopy. Basic measurements were performed at the day 2, 6, 9, 16, 23 and 30 of storage and additionally 2-3 days after it. Topologicaldefects occurred on RBC membranes by day 9. They appeared as domains with grain-like structures ("grains") sized up to 200. nm. These domains were appeared in almost all cells. Later these domains merged and formed large defects on cells. It was the formation of domains with the "grains" which was onset process leading eventually to destruction of PRBC. Possible mechanisms of transformation of PRBC and their membrane are related to the alterations of spectrin cytoskeleton. During this storage period potassium ions and lactat concentrations increased, pH decreased, intracellular concentration of reduced glutathione diminished in the preservative solution. Changes of PRBC morphology were detected within the entire period of PRBC storage. Discocytes predominated at the days 1 and 2. By day 30 PRBC transformed into irreversible echinocytes and spheroechinocytes. Study of defects of membranes nanostructure may form the basis of assessing the quality of the stored PRBC. This method may allow to work out the best recommendations for blood transfusion. © 2015 Elsevier Inc.

PubMed | Moscow State University and Va Negovsky Scientific Research Institute Of General Reanimatology
Type: Journal Article | Journal: International journal of pharmaceutics | Year: 2016

Here we show that methemoglobin is converted to oxyhemoglobin in the presence of perfluorocarbon (PF) emulsion. Methemoglobin in blood at the level of above 30% can cause severe complications and lethal outcome. Some pharm chemicals in blood in vivo and in vitro can lead to oxidation of iron, Fe(2+)Fe(3+), and to increased level of methemoglobin. The oxidized heme is not able to carry oxygen, hypoxia arises and irreversible changes are developing in vital organs. We added NaNO2 solution in different concentrations to blood in vitro in order to yield methemoglobin. Then the suspension of PFC nanoparticles was added. As methemoglobin interacted with PFC nanoparticles the optical density of peaks typical for oxyhemoglobin increased and spectral peak of methemoglobin decreased. The greater the concentration of PFC and the more was the incubation time, the more efficient was the process of reduction of methemoglobin to oxyhemoglobin. We proved experimentally that with an initial concentration of methemoglobin in average 95% the addition of nanoparticles of PFC decreases its concentration to 9% in average. At the same time the concentration of oxyhemoglobin increased in average from 5% to 81%.

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