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Shi Y.,AlloSource | Niedzinski J.R.,LABs Inc. | Samaniego A.,AlloSource | Bogdansky S.,AlloSource | Atkinson B.L.,AlloSource
Tissue Engineering - Part A | Year: 2012

Mesenchymal stem cells (MSCs) isolated from cadaveric adipose tissue can be obtained in large quantities, and have been reported in the literature to be capable of inducing bone formation in vivo and ex vivo.1-6 The hypothesis tested whether a demineralized cancellous bone matrix (DCBM) can provide an effective substrate for selection and retention of stem cells derived from the stromal vascular fraction (SVF) of adipose. Human cadaveric adipose tissue was recovered from a donor and digested. The resulting SVF-containing MSCs were seeded onto the demineralized bone allografts, after which the nonadherent cells were washed off. The MSCs were characterized using a flow cytometer and tri-lineage differentiation (osteogenesis, chondrogenesis, and adipogenesis) in vitro. The stem cell-seeded allografts were also characterized for cell number, adherence to the DCBM, osteogenic activity (alkaline phosphatase and Alizarin Red staining), and bone morphorgenic protein (BMP) quantity. Flow cytometry identified a mean total of 7.2% MSCs in SVF and 87.2% MSCs after culture. The stem cells showed the capability of differentiating into bone, cartilage, and fat. On the 21 stem cell-seeded bone allografts, there were consistent, attached, viable cells (100,744±22,762 cells/cube). An assessment of donor age, gender, and body mass index revealed no significant differences in cell numbers. Enzyme-linked immunosorbent assay revealed the presence of BMP-2 and BMP-7. In conclusion, this bone graft contains three key elements for bone regeneration: adhered osteogenic stem cells, 3D osteoconductive bone scaffold, and osteoinductive BMP signal. It therefore has the potential to be effective for bone regeneration. © 2012 Mary Ann Liebert, Inc.

Koprivnjak O.,University of Rijeka | Dminic I.,Polytechnic of Rijeka | Kosic U.,Polytechnic of Rijeka | Majetic V.,University of Rijeka | And 2 more authors.
European Journal of Lipid Science and Technology | Year: 2010

In order to evaluate the role of intrinsic characteristics of olive cultivars on dynamics of oil quality parameters changes related to the olive fruit fly (Bactrocera oleae (Gmelin)) attack, two specific olive cultivars were compared, Istarska bjelica and Buža. I. bjelica is characterised by late ripening, a lower flesh/stone mass ratio and higher oleic/linoleic ratio, the total phenols content and oil mass fraction compared to Buža. Oil samples were obtained at two harvesting dates, from fruit lots with 0, 25, 50, 75 and 100%of infestation degree, expressed by mass. Oil and water content in olive paste, as well as total phenols, fatty acids composition and standard chemical and sensorial oil quality parameters were determined. At equal maturity level, the cultivar with a higher oil (24± 1% vs. 16± 1%) and lower water content (54± 2% υs. 61± 4%) was more susceptible to hydrolytic degradation, while the one with the lower total phenols content (231± 32 vs. 575± 124 mg/kg) and oleic/linoleic fatty acid ratio (5.7 υs. 9.1) was more liable to oxidative deterioration of oil. Lower susceptibility to negative flavours formation could be related to the absence of ripening induced by fly attack and less favourable environment for larvae growth (higher total phenols content, lower water content and flesh/stone mass ratio). Practical applications: Understanding the impact of olive fly attack is the basis of scientific and practical interest in the production of olives and olive oil. Different intrinsic characteristics of olive cultivars could lead to different susceptibility to oil quality deterioration caused by olive fly attack, which opens a possibility to adjust and minimise pesticide treatments. Knowledge about this topic could contribute to the reduction of costs of olive pests control, as well as to the improvement of quality and food safety concept in olive oil production. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Butinar B.,University of Primorska | Bucar-Miklavcic M.,University of Primorska | Bucar-Miklavcic M.,LABs Inc. | Mariani C.,Stazione Sperimentale per le Industrie degli Oli e dei Grassi | Raspor P.,University of Ljubljana
Food Chemistry | Year: 2011

The Štajerska region in north-eastern Slovenia and the Styria region in southern Austria have a long tradition of growing pumpkins (Cucurbita pepo L.) as an oil crop. GC-MS determination of the free and esterified minor compounds in oil of roasted pumpkin seeds from the Slovenian C. pepo L. variety 'Slovenska golica' revealed the presence of two previously unreported compounds: alpha-tocomonoenol and gamma-tocomonoenol. Using the GC-MS data, reference samples (Crude Palm Oil) and tocopherol and tocotrienol standards it was possible to assign and quantify alpha-tocomonoenol (17.6 ± 0.6 μg/g) and gamma-tocomonoenol (118.7 ± 1.0 μg/g) compounds in roasted 'S. golica' seed oil using HPLC. The concentrations of alpha-tocopherol and gamma-tocopherol were 77.9 ± 1.9 μg/g and 586.0 ± 4.6 μg/g, respectively. Surprisingly the gamma-tocotrienol concentration found was only 6.9 ± 0.2 μg/g. Analysis of the seeds from which the oil was pressed showed the initial gamma-tocotrienol amount was even lower (1.6 ± 0.1 and 2.2 ± 0.1 μg/g in the ground and roasted seeds, respectively) than in the roasted seed oil. © 2011 Elsevier Ltd. All rights reserved.

Germek V.M.,University of Rijeka | Koprivnjak O.,University of Rijeka | Butinar B.,University of Primorska | Pizzale L.,University of Udine | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

The influence of the phenolic content in olive paste of cv. Buža increased by the addition of an aqueous solution of phenolic extract of freeze-dried olive pulp (cv. Istarska bjelica) on the final products of the lipoxygenase pathway in oil was studied. Increases by 12, 38, and 56% for ripe fruits (maturity index = 4.0) and by 38% for unripe fruits (maturity index = 1.2) were examined. Phenols in the olive paste were determined according to the HPLC method, whereas volatiles in oil were determined according to SPME-GC-MS. A significant negative effect on Z-3-hexenal and E-2-hexen-1-ol (Tukey's test, p < 0.05) was found for ripe fruits (average decreases of 55 and 60%, respectively), but not for the unripe sample. Positive effects in both ripening levels were found for Z-3-hexenyl acetate (average increase of 68% for ripe and a double increase for unripe fruits) and total C5 compounds (average increase of 32% for ripe and an increase of 30% for unripe fruits). © 2013 American Chemical Society.

Jung H.-J.,University of Pittsburgh | Jung H.-J.,Chung - Ang University | Vangipuram G.,University of Pittsburgh | Fisher M.B.,University of Pittsburgh | And 5 more authors.
Journal of Orthopaedic Research | Year: 2011

Soft tissue allografts, such as the bone-patellar tendon-bone (BPTB) graft, have been frequently used for anterior cruciate ligament (ACL) reconstruction. As allografts are subjected to freezing and thawing for multiple cycles, the objective of this study was to measure the changes of the biomechanical properties of the human BPTB allograft after 4 and 8 freeze-thaw cycles in comparison to a single freeze-thaw cycle. Three BPTB specimens were procured from 21 human donors and divided into three groups: 1, 4, or 8 freeze-thaw cycles. Each freeze-thaw cycle consisted of freezing at -20 ± 10°C for more than 6 h and thawing at 22 ± 3°C for at least 6 h. Tensile testing of the BPTB specimens consisted of loading between 50 N and 250 N for 100 cycles and then loading to failure. Cyclic loading revealed a similar amount of creep (∼0.5 mm) among the three freeze-thaw cycles groups (p = 0.38). The stiffness of the BPTB graft for the 1, 4, and 8 freeze-thaw cycle groups were 244 ± 42 N/mm, 235 ± 39 N/mm, and 231 ± 40 N/mm, respectively (p = 0.43). Similar findings were obtained for the ultimate load of the BPTB graft (p = 0.14) and the tangent modulus of the PT substance (p = 0.41). The results of this study suggest that there would be little measurable effect on the structural properties of the BPTB graft or mechanical properties of the PT tissue substance following 8 freeze-thaw cycles. These BPTB allografts could potentially be re-frozen without a loss in their biomechanical properties, given appropriate storage and care. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc.

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