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Dhama K.,Indian Veterinary Research Institute | Karthik K.,Indian Veterinary Research Institute | Chakraborty S.,Pt. Nehru Complex | Tiwari R.,Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu | And 3 more authors.
Pakistan Journal of Biological Sciences | Year: 2014

Diagnosis is an important part in case of animal husbandry as treatment of a disease depends on it. Advancement in molecular biology has generated various sophisticated tools like Polymerase Chain Reaction (PCR), its versions along with pen-side diagnostic techniques. Every diagnostic test however has both advantages and disadvantages; PCR is not an exception to this statement. To ease the odds faced by PCR several non-PCR techniques which can amplify DNA at a constant temperature has become the need of hour, thus generating a variety of isothermal amplification techniques including Nucleic Acid Sequence-Based Amplification (NASBA) along with Self-Sustained Sequence Replication (3SR) and Strand Displacement Amplification (SDA) and Loop mediated isothermal amplification (LAMP) test. LAMP stands out to be a good and effective diagnostic test for empowering in developing countries as it does not require sophisticated equipments and skilled personnel and proves to be cost-effective. Performance of LAMP mainly relies on crafting of six primers (including 2 loop primers) ultimately accelerating the reaction. LAMP amplifies DNA in the process pyrophosphates are formed causing turbidity that facilitates visualisation in a more effective way than PCR. The Bst and Bsm polymerase are the required enzymes for LAMP that does not possess 5'-3' exonuclease activity. Results can be visualized by adding DNA binding dye, SYBR green. LAMP is more stable than PCR and real-time PCR. Non-involvement of template DNA preparation and ability to generate 109 copies of DNA are added benefits that make it more effective than NASBA or 3SR and SDA. Thus, it fetches researcher's interest in developing various versions of LAMP viz., its combination with lateral flow assay or micro LAMP and more recently lyophilized and electric (e) LAMP. Availability of ready to use LAMP kits has helped diagnosis of almost all pathogens. LAMP associated technologies however needs to be developed as a part of LAMP platform rather than developing them as separate entities. This review deals with all these salient features of this newly developed tool that has enlightened the world of diagnosis. © 2014 Asian Network for Scientific Information. Source


Kumar A.,National Institute of Animal Health | Singh S.V.,11 Health | Srivastava A.K.,Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu | Gangwar N.K.,Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu | And 6 more authors.
Journal of Biological Sciences | Year: 2014

Johne's Disease (JD), caused by Mycobacterium avium subspecies paratuberculosis, is endemic in domestic animals and adversely affects dairy industry worldwide. In the present study, efficacies of 'Indigenous'and commercial (Gudair, Spain) vaccines were evaluated for control of JD in experimentally challenged goats. Goats were grouped into Sham-immunized, Indigenous and Gudair vaccine groups. Vaccinated kids were challenged at 50 and 270 Days Post Vaccination (DPV), with 3×109 and 5×109 'Indian Bison Type strain 'S 5', respectively and sacrificed at 150 and 450 DPV after 1st and 2nd challenge, respectively. Vaccines were evaluated for improvements in physical condition (diarrhea, weakness, body coat color), clinical symptoms (shedding of bacilli, mortality, morbidity), immune responses (cell-mediated and humoral), pathology (gross and microscopic lesions) and production status (body weights, growth rates). Vaccinated goats gained higher body weights vis a vis sham-immunized. Mortality was higher in sham-immunized. Cell Mediated Immunity (CMI) response increased at 30 DPV and showed down regulation from 90 DPV onwards in vaccinated goats. Significant increase in humoral immune response was observed in vaccinated goats at 180 DPV and maintained till 450 DPV. Microscopical examination at 180 DPV showed reduced shedding in vaccinated groups, At 200 DPV, group 1 goats showed thickening of small intestine with corrugations specifically at ileocaecal junction, catarrhal enteritis with infiltration of mononuclear cells and epitheloid cells. In vaccinated groups, there were focal thickening of intestines at 450 DPV with lesions of chronic catarrhal enteritis and presence of lymphocyte, plasma cells and macrophages cells with a few epitheloid cells. Monitoring of MAP DNA in the blood of experimental goats of all the groups was done by testing of blood samples by Polymerase Chain Reaction (PCR) and the vaccinated groups of goats revealed MAP bacilli free status upto 300 DPV. Both the vaccines provided protection after challenge I, but since indigenous vaccine also protected goats after challenge II, was therefore superior. In conclusion, the indigenous vaccine must be exploited for its full potential for effective prevention and control of this economically important disease having public health concerns. © 2014 Asian Network for Scientific Information. Source


Mahima,Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu | Singh K.V.,Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu | Verma A.K.,Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu | Kumar V.,Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu | And 2 more authors.
Pakistan Journal of Biological Sciences | Year: 2013

The 'Hariana' breed of cattle has been proved to be highly adaptable to harsh climatic conditions and resistant to common diseases prevalent in India. In this study, the normal reference values of hematological and serum proteins and other blood biochemical parameters were determined in the heifers of Hariana breed maintained at Instructional livestock farm complex, DUVASU, Mathura, India. A total of twenty four animals were used in this study. Blood was taken aseptically from all the animals and transported to laboratory for hematological and biochemical analysis. The hematological parameters (Hemoglobin, total erythrocyte count, total leukocyte count, packed cell volume) and biochemical parameters (Total protein, total albumin, albumin globulin ratio, urea, creatinine, calcium, phosphorous, calcium phosphorous ratio, AST, ALT) values were statistically analyzed, mean and standard deviations were calculated and set as reference values. This study reported hematological and serum biochemical values which could serve as baseline information for comparison in conditions of nutrient deficiency, physiological and health status of Hariana cattle heifers in India. © 2013 Asian Network for Scientific Information. Source

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