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Singh A.,Uttar Pradesh Pandit Deen Dayal Upadhayay | Gupta V.K.,Uttar Pradesh Pandit Deen Dayal Upadhayay | Kumar A.,Uttar Pradesh Pandit Deen Dayal Upadhayay | Singh V.K.,Uttar Pradesh Pandit Deen Dayal Upadhayay | And 2 more authors.
Indian Journal of Animal Sciences | Year: 2014

The present study analyses the sensitivity and specificity of OMP31 gene based amplification directly from sera samples. Sero-positive animals (73) in rOmp31 i-ELISA were subjected to Omp31 gene amplification from sera samples and only 40 samples amplified 723kb product suggesting these as true positive animals. When similar test was applied with the sera samples of 16 infected (culture positive) and 20 non-infected (culture negative) goats, all the culture positive animals revealed 723 kb product while none of the culture negative goats revealed amplification. Hence serum based PCR appeared to be 100% sensitive and specific in truly infected animals. Thus serum is the optimal specimen for the diagnosis of brucellosis and leads to simplification of assay and shortens turnaround time. Source


Tiwari R.,Uttar Pradesh Pandit Deen Dayal Upadhayay | Dhama K.,Indian Veterinary Research Institute | Chakraborty S.,Pt. Nehru Complex | Kumar A.,Uttar Pradesh Pandit Deen Dayal Upadhayay | And 2 more authors.
Pakistan Journal of Biological Sciences | Year: 2014

Since the discovery of bacteriophages at the beginning of the 19th century their contribution to bacterial evolution and ecology and use in a variety of applications in biotechnology and medicine has been recognized and understood. Bacteriophages are natural bacterial killers, proven as best biocontrol agents due to their ability to lyse host bacterial cells specifically thereby helping in disease prevention and control. The requirement of such therapeutic approach is straight away required in view of the global emergence of Multidrug Resistant (MDR) strains of bacteria and rapidly developing resistance to antibiotics in both animals and humans along with increasing food safety concerns incuding of residual antibiotic toxicities. Phage typing is a popular tool to differentiate bacterial isolates and to identify and characterize outbreak-associated strains of Salmonella, Campylobacter, Escherichia and Listeria. Numerous methods viz. plaque morphology, ultracentrifugation in the density gradient of CsCl2, and random amplified polymorphic DNA (RAPD) have been found to be effective in detection of various phages. Bacteriophages have been isolated and recovered from samples of animal waste products of different livestock farms. High titer cocktails of broad spectrum lytic bacteriophages are usually used for clinical trial for assessing their therapeutic efficacy against antibiotic unresponsive infections in different animals. Bacteriophage therapy also helps to fight various bacterial infections of poultry viz. colibacillosis, salmonellosis and listeriosis. Moreover, the utility of phages concerning biosafety has raised the importance to explore and popularize the therapeutic dimension of this promising novel therapy which forms the topic of discussion of the present review. © 2014 Asian Network for Scientific Information. Source


Dhama K.,Indian Veterinary Research Institute | Chakraborty S.,Pt. Nehru Complex | Tiwari R.,Uttar Pradesh Pandit Deen Dayal Upadhayay | Barathidasan R.,Indian Veterinary Research Institute | And 2 more authors.
Pakistan Journal of Biological Sciences | Year: 2013

Fungal/mycotic diseases cause significant economic losses to the poultry industry either due to their direct infectious nature or due to production of mycotoxins, the secondary fungal metabolites produced in grains or poultry feed. Several fungi have created havoc in the poultry industry and some of them cause direct harm to human health due to their zoonotic implications. They are responsible for high morbidity and mortality, especially in young birds and cause stunted growth and diarrhea; and fatal encephalitis. Mycotic dermatitis is a possible health hazard associated with poultry houses. Mycotoxins are the leading cause of producing immunosuppression in birds, which makes them prone to several bacterial and viral infections leading to huge economic losses to the poultry industry. In comparison to bacterial and viral diseases, advances in diagnosis, treatment, prevention and control of fungal diseases in poultry has not taken much attention. Recently, molecular biological tools have been explored for rapid and accurate diagnosis of important fungal infections. Effective prevention and control measures include: appropriate hygiene, sanitation and disinfection, strict biosecurity programme and regular surveillance/monitoring of fungal infections as well as following judicious use of anti-fungal drugs. Precautionary measures during crop production, harvesting and storing and in feed mixing plants can help to check the fungal infections including health hazards of mycotoxins/mycotoxicosis. The present review describes the fungal pathogens causing diseases in poultry/birds, especially focusing to their diagnosis, prevention and control measures, which would help in formulating appropriate strategies to have a check and control on these unwanted troubles to the poultry producers/farmers. © 2013 Asian Network for Scientific Information. Source

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