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Metropolitan Government of Nashville-Davidson (balance), TN, United States

Estes A.-M.,UTHSC Memphis | McAllen K.,UTHSC Memphis | Parker M.S.,University of Memphis | Sah R.,University of Cincinnati | And 6 more authors.
Amino Acids | Year: 2011

Treatment of CHO cells expressing human Y receptors (Y1, Y 2 or Y4 subtype) with pertussis toxin results in a large decrease in functional receptors, with a preferential loss of heteropentameric assemblies of receptor dimers and G-protein trimers. This occurs in parallel to inactivation of the nucleotide site of Gi α subunits, with a half period of about 4 h. The loss could be mainly due to proteolysis at the level of recycling/ perinuclear endosomes, and of receptor completion in the ER, since it is reduced by co-treatment with ammonium chloride, an inhibitor of particulate proteinases. Antagonists do not strongly decrease the heteropentameric fraction. These findings indicate that the upkeep of Y receptor dimers in epithelial cell lines depends on the association of receptor oligomers with functional Gi α subunits. This interaction could use the juxtamembrane helix 8 in the fourth intracellular domain, and could also be supported by the C-terminal helix of the third intracellular loop, as outlined in the companion review (Parker et al., Amino Acids, doi: 10.1007/s00726-010-0616-1, 2010). © 2010 Springer-Verlag.

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