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Cerna M.,Ustav Technology A Mikrobiologie Potravin | Kracmar S.,Ustav Biochemie A Analyzy Potravin
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2010

The aim of this study was to investigate the effect of storage duration on amino acids content in potato tubers. Tubers of six cultivars were stored for 16 weeks. Crude protein was determined by the Kjeldahl method. The amino acid content was identified after acid hydrolysis; sulphur amino acids were oxidized with the mixture of hydrogen peroxide, formic acid and phenol. The essential amino acids index and chemical score was calculated. The whole egg protein was used as reference protein. The effect of storage duration and cultivar on the total amino acids content was found to be significant in all potato varieties. The level of the total amino acids contents was determined within the range of 8087%. Similar downward trend was observed in crude protein. The essential amino acids index varied considerably among the potato cultivars, ranging from 42 to 57%. Sulfur amino acids and isoleucin were the limiting amino acids in all the investigated tubers.

Galik B.,Katedra vyzivy zvierat | Simko M.,Katedra vyzivy zvierat | Juracek M.,Katedra vyzivy zvierat | Biro D.,Katedra vyzivy zvierat | And 3 more authors.
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2012

The aim of this study was to monitor levels of minerals in mare's colostrum in the fi rst 3 days after foaling. Colostrum samples from four warm-blood mares (in three repetitions) were analysed 2, 12, 24, 36, 48 and 72 hours postpartum. The samples were obtained by hand milking. Calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na), potassium (K), zinc (Zn), copper (Cu), iron (Fe) and manganese (Mn) contents were analysed in each sample. Non-signifi cant decrease of postpartum Ca and Na contents was found in the analysed samples. Signifi cant diff erences (P < 0.05) in P content were detected between 2 and 36 hours postpartum. Content of Mg showed statistically signifi cant diff erences (P < 0.05) during the period after foaling (2, 24, 36, 48 and 72 hours post foaling). Mg concentration reached its maximum (21.8 g.kg -1 of dry matter) 2 hours postpartum and it dropped to its lowest level (5.27 g.kg-1 of dry matter) 48 hours after foaling. Similarly, the highest K content (signifi cant in relation to values obtained 36, 48 and 72 hours postpartum) was detected 2 hours after foaling while the lowest K level (37.96 g.kg-1 of dry matter) was found 48 hours postpartum. As regards the microelements, Cu showed insignifi cant diff erences in its postpartum levels (P > 0.05). Besides growth of Fe levels, decrease of Zn and Mn (P > 0.05) was also observed.

Hrivna L.,Mendel University in Brno | Pechkova J.,Mendel University in Brno | Buresova I.,Ustav Technology A Mikrobiologie Potravin
Listy Cukrovarnicke a Reparske | Year: 2014

In the years 2011 and 2012, a small plot field experiment was set up monitoring the influence of boron application on yield and technological quality of sugar beet. The yield of leaves was almost twice higher in 2011 (62.6 t ha-1). On the other hand, the yield of roots was higher in 2012 (107.6 t ha-1). Also in 2012, higher average sugar content (19.41 %) was observed. More favorable, lower values of soluble ash were observed in 2011 (0.57 %). Among the tested fertilizers, Bortrac has proven to be most effective. Good results were also achieved by the application of fertilizer CARBONBOR.

Moravcikova D.,Institute Zdravotnickych Studii | Kucekova Z.,Zdenka Kucekova | Mlcek J.,Ustav Technology A Mikrobiologie Potravin | Rop O.,Ustav Technology A Mikrobiologie Potravin | Humpolicek P.,Centrum polymernich materialu Fakulta technologicka
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2012

During past decades a lot of experimental studies shown that polyphenols have anti-carcinogenic properties have been published. Their antioxidant and tumour arresting effects have been demonstrated using both in vitro and in vivo studies many times. Aim of the present study was to investigate the content of polyphenols in edible flowers of Ag yoncha, Wild chive, Meadow salsify and Garden sorrel and their effect on proliferation activity of human hepatocellular carcinoma cells which has not been studied yet. Anti-proliferative effect was evaluated in vitro using following concentrations of polyphenols 100, 75, 50 and 25μg/ml. Also, phenolic composition was determined by the high performance liquid chromatography The present results indicate that even low concentrations of edible flowers' polyphenols inhibited cell proliferation significantly. This effect was observed at all studied edible flowers extracts and was independent on the compositions of individual polyphenols. Results indicate possible employment of studied edible flowers for prevention as well as for treatment of cancers.

Balik J.,Mendel University in Brno | Rop O.,Ustav Technology A Mikrobiologie Potravin | Mlcek J.,Ustav Technology A Mikrobiologie Potravin | Hic P.,Mendel University in Brno | And 2 more authors.
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis | Year: 2012

Although there are tens of diff erent apple varieties in Europe, only some of them were purposefully bred and selected in the past. In spite of the fact that they can have outstanding nutritional and technological properties, the majority of them are of only a local importance. The objective of this study was to show and popularize altogether 35 local apple varieties which are typical of the conditions of Central Europe. However, their genetic uniqueness represents an irreplaceable ecological wealth and for that reason these local varieties could become a new and outstanding source of nutrients and food. Today, they can be used not only for direct consumption and in food industry but also as a potential material for further breeding and selection. It was found out in our experiments that the highest content of total acids was shown by the variety 'Citronové zimní' (6.1 g.kg-1 of fresh matter). As far as the content of pectins was concerned, the highest levels were found out in the variety 'Strymka' (3.26% in fresh matter). Of minerals, potassium showed the highest levels in fruit; for example, the variety 'Boikovo' contained 9.70 ppm of this element in dry matter. Peels showed approximately 7 times higher antioxidant capacity than the flesh. For example, peels of the variety 'Bernské růžové' showed antioxidant capacity of as much as 56.65 mmol of trolox per kg of fresh matter. This paper should contribute to efforts focused on saving of genetic variability of apples and their further application both in human nutrition and a further breeding and selection under conditions of pomological and alimentary practice.

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