Ustav imunologie a alergologie

Plzeň, Czech Republic

Ustav imunologie a alergologie

Plzeň, Czech Republic
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Belohlavkova S.,Immuno flow Sro | Kopelentova E.,Ambulance Alergologie A Klinicke Imunologie | Setinova I.,Ustav Imunologie 2. LF UK | Visek P.,Immunia | And 15 more authors.
Alergie | Year: 2015

Food allergy prevalence has increased in recent years. Currently, it is described to be present in 7% of children and 5% of adults. The spectrum of most frequent allergens has changed and widened, symptoms are becoming more severe. There are significant geographical differences in the incidence of food allergy, types of senzitization and symptoms. Accordingly, the most common causes of food anaphylaxis are different in respective regions. No recent relevant epidemiological data on occurence of food allergy in Czech Republic are available. DAFALL registry (Database of Food Allergies) has been founded on iniciative of Immuno-flow, s.r.o., to collect data from patients with newly diagnosed food allergy. In this report we present results from 275 patients enrolled into the registry during the first six months of its existence.

Vlas T.,Ustav imunologie a alergologie | Merglova L.,Hematologicko onkologicke Oddeleni | Panzner P.,Ustav imunologie a alergologie | Hanzlikova J.,Ustav imunologie a alergologie | And 2 more authors.
Alergie | Year: 2011

Mesenchymal stem cells stand in the focus of interest of a lot of medicine branches. Our study is focused on their immunomodulatory properties. We tested the influence of their presence on nonspecific activation of lymphocytes with phytohemagglutinin (PHA) during the lymphocyte transformation test. Adding of MSC into the cultivation medium significantly decreased the level of lymphocyte activation, which was measured by the cell-cycle analysis by flow cytometry. The number of activated cells in the G2/M phase was decreased by 19% (median, p < 0,01). Insignificant proliferation caused by MSC alone was detected in the culture of non-stimulated lymphocytes - increase of proliferating lymphocytes by 0,8% (ns). Monitoring changes in cultures we watched increased viability in cultures with added MSC. The level of fragmented DNA in these samples was by 2,3% lower (p = 0,029) than in the samples without MSC. The results confirm low immunogenicity of MSC, their ability to decrease lymphocyte activation and support inducing of immunotolerance. MSC may become a basis for new medical approaches to the treatment of some immunopathological states (autoimmune disorders, GVHD etc.).

Havel D.,Klinika Pneumologie a Ftizeologie | Trhlikova S.,Klinika Pneumologie a Ftizeologie | Pesek M.,Klinika Pneumologie a Ftizeologie | Vachova M.,Ustav Imunologie a Alergologie | And 4 more authors.
Studia Pneumologica et Phthiseologica | Year: 2017

Presented is a case of a patient after bone marrow transplantation for acute lymphoblastic leukemia. She developed graft-versus-host-disease and was diagnosed with immunodeficiency. During the follow-up, she developed bronchiectasis and her condition slowly deteriorated. Subsequently, a large bulla was detected in her left upper lobe; in the inoperable patient with severe lung function impairment, the finding was managed atypically, but with an excellent effect, by implanting an endobronchial valve. The article discusses lung manifestations of chronic graft-versus-host-disease and the origin of bronchiectasis and the air space in the left upper lobe. We underline the necessity for multidisciplinary cooperation in managing such complicated conditions including the role of a pulmonologist in the diagnosis and therapy of hemato-oncological patients. Selected recommendations are mentioned.

Panzner P.,Ustav imunologie a alergologie | Vachova M.,Ustav imunologie a alergologie | Vitovcova P.,Ustav imunologie a alergologie | Vlas T.,Ustav imunologie a alergologie | Maly M.,Statni zdravotni ustav Prague
Alergie | Year: 2014

Molecular diagnosis of allergy is a modern approach which enables new insights into the sensitization profiles of allergic patients. Microarray technology provides detection of sensitization to a higher number of molecules in frame of one test. We used this tool in the sensitization analysis to pollen derived molecules in patients coming from our region. 1096 patients sensitized to at least one pollen derived molecule were included into the analysis. The most frequent sensitization rate (80.8%) was found to grass pollen derived molecules (Phl p 1 66.3%), the second most frequent (54.9%) was to betulaceae pollen derived molecules (Bet v 1 52.5%). Sensitization rates to cupressaceae and plane tree pollen derived molecules were observed unexpectedly frequently (in 14.0%, resp. 15.3%). Sensitization rates to other pollen derived molecules were in the expected ranges. Sensitizations to cross-reacting molecules (panallergens) were observed less frequently than reported from southern countries. Molecular diagnosis of allergy gives a more precise and comprehensive insight into the sensitization profile of a patient, allowing better understanding of the sensitization process, and the explored data may help to the optimization of allergen specific immunotherapy.

Vitovcova P.,Ustav Imunologie a Alergologie | Panzner P.,Ustav Imunologie a Alergologie | Malkusova I.,Ustav Imunologie a Alergologie | Vlas T.,Ustav Imunologie a Alergologie
Alergie | Year: 2014

Peanut allergy is one of the most common food allergies and may cause severe reactions. Skin prick tests and specific IgE antibodies to peanut extract are not able to distinguish between symptomatic and asymptomatic patients. Reactivity todistinct peanut allergen components may draw attention to the potencial risk of more severe reactions.Method: We analyzed the group of 294 patients sensitized to peanut allergens: Ara h1, Ara h 2, Ara h 3 or Ara h 8. Patients were divided into 8 groups according to their dominant clinical symptoms and frequency of sensitization to individual allergen components in each group was assessed.Results: Asymptomatic patients and patients with oral allergy syndrome were mostly adults and sensitized to Ara h 8. More than 90% of these patients were sensitized to birch pollen. More severe reaction were observed mostly in children, these kinds of reactions were related to polysensitization and sensitization to seed storage proteins.Conclusion: We confirmed, that allergy to seed storage proteins, especially Ara h 2, is frequently responsible for the more severe allergy symptoms. Sensitization to Ara h 8 is frequently associated with mild or no symptoms.

In patients with a long history of angioedema without urticaria was diagnosed hereditary angioedema (HAE) and launched a long-term prophylactic treatment with attenuated androgens danazol. The patient was at the time of diagnosis of HAE, unfortunately, also treated with ACE inhibitors for hypertension. After initiation of the therapy with danazol, and simultaneously change in therapy of hypertension with discontinuation of ACE inhibitors, the status of the patient improved markedly in recent years and symptoms of HAE appeared sporadically only during infection or following surgery. Since 2001 the patient after wasp or bee sting had a large edema and patient thought that these are attacks of HAE. However at the turn of the millennium the patient had an anaphylactic reaction after a Hymenoptera insect sting and in allergy testing were confirmed sensitization to Hymenoptera venom and we started the treatment with allergen immunotherapy. Conclusion: Patients with HAE have discontinued treatment with ACE inhibitors because ACE inhibitors trigger angioedema. Patients with HAE sometimes poorly differentiated between HAE attack and allergy attack with similar clinical manifestations. The possibility of coincidence of two such serious diagnoses must be considered.

Vachova M.,Ustav Imunologie a Alergologie | Panzner P.,Ustav Imunologie a Alergologie | Vlas T.,Ustav Imunologie a Alergologie
Alergie | Year: 2012

Insect sting is one of the most often cause of anaphylaxis, which with its unpredictable, acute and potentially life-threatening course, represents a serious medical problem. Proper diagnosis and treatment of hymenoptera venom allergy is therefore extremely important and the key role belongs into hands of experienced allergist. EAACI position paper on the diagnosis of hymenoptera venom allergy from 2005 is still valid. Precise personal history, skin testing and the determination of specific IgE antibodies to bee and wasp venoms remains the gold standard of examination. The definite differentiation between bee and wasp venom allergy is often complicated by frequently observed double positivity of commonly used test results, which complicate the choice of relevant venom for immunotherapy. For precise diagnosis in patients, with double positivity of the test results, basophile activation test and component resolved diagnosis are used. In the field of hymenoptera venom allergy, the component resolved diagnosis is the most up to date diagnostic method, which is the object of intensive research and is quite promising for the future.

Gutova V.,Ustav imunologie a alergologie
Pediatrie pro Praxi | Year: 2016

The risk factors for asthma and its exacerbations in childhood are discussed. The genetic factors and environmental risk factors. These are biogenic, including influence of allergens - house dust mites, animal allergens, pollen and mould allergens, microbial - bacterial and viral. Environmental risk factors - industrial and traffic pollution, tobacco smoke exposure, risk factors of inappropriate alimentation, obesity. The preventive measures of particular risk factors are considered, primary, secondary and tertiary prevention. Paediatricians, allergists and pneumologists should strive for a greatest possible prevention of allergic and nonallergic asthma.

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