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Liu W.-B.,PLA Fourth Military Medical University | Zhou J.,Urumqi General Hospital of PLA | Zhou J.,PLA Fourth Military Medical University | Qu Y.,PLA Fourth Military Medical University | And 5 more authors.
Neurochemistry International | Year: 2010

Background:: The 1-methyl-4-phenylpyridinium ion (MPP+), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it causes a severe Parkinson's disease-like syndrome accompanied by increased levels of intracellular reactive oxygen species (ROS) and apoptotic death. In the present study, we investigated the protective effects of osthole, a coumarin compound extracted from the plant-derived medicine Cnidium monnieri, on MPP+-induced cytotoxicity in cultured rat adrenal pheochromocytoma (PC12) cells. Methods:: PC12 cells were treated with MPP+ 2h after treated with different concentrations of osthole. 24h later, the cell viability, the release of lactate dehydrogenase, the activity of caspase-3 and cytochrome c, the expression ratio of Bax/Bcl-2 and the generation of intracellular ROS were detected. Results:: We found that pretreatment with osthole on PC12 cells significantly reduced the loss of cell viability, the release of lactate dehydrogenase, the activity of caspase-3 and cytochrome c, the increase in Bax/Bcl-2 ratio and the generation of intracellular ROS induced by MPP+. Moreover, our HPLC analysis of cell extracts confirmed that extracellular osthole does penetrate the cell membrane. Thus osthole may function as an intracellular antioxidant to reduce oxidative stress induced by MPP+. Conclusions:: Therefore, the present study supports the notion that osthole may be a promising neuroprotective agent for the treatment of neurodegenerative disorders such as Parkinson's disease. © 2010 Elsevier Ltd.


Chao X.,PLA Fourth Military Medical University | Zhou J.,Urumqi General Hospital of PLA | Chen T.,PLA Fourth Military Medical University | Liu W.,PLA Fourth Military Medical University | And 6 more authors.
Brain Research | Year: 2010

Osthole, a natural coumarin derivative, has taken considerable attention because of its diverse pharmacological functions. It has been reported to be useful in the treatment of chronic cerebral hypoperfusion and neuronal damage. In the present study, we examined the neuroprotective effect of osthole and its potential mechanisms against acute ischemic stroke induced by middle cerebral artery occlusion (MCAO) in rats. The rats were pretreated with osthole 10, 20 and 40 mg/kg 30 min before MCAO. The neuroprotective effect of osthole against acute ischemic stroke was evaluated by neurological deficit score (NDS), dry-wet weight and 2,3,5-triphenyltetrazolium chloride (TTC) staining. The contents of malondialdehyde (MDA) and glutathione (GSH), activity of myeloperoxidase (MPO) and the level of interleukin (IL)-1β and IL-8 after 2 h of MCAO in rats were detected to investigate its anti-oxidative action and anti-inflammatory property. Pretreatment with osthole significantly increased in GSH, and decreased the volume of infarction, NDS, edema, MDA, MPO, IL-1β and IL-8 compared with rats in the MCAO group at 24 h after MCAO. The study suggests the neuroprotective effect of osthole in the MCAO model of rats. The anti-oxidative action and anti-inflammatory property of osthole may contribute to a beneficial effect against stroke. © 2010 Elsevier B.V. All rights reserved.


Zhou J.,Urumqi General Hospital of PLA | Zeng P.,Urumqi General Hospital of PLA | Cheng Z.H.,Urumqi General Hospital of PLA | Liu J.,Urumqi General Hospital of PLA | And 2 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2011

A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction has been developed and used to quantify the osthole in cerebral ischemia reperfusion rat plasma following oral administration. The analysis was performed by reversed phase high performance liquid chromatography with fluorescence detection. Extraction conditions such as solvent identity, agitation rate, salt concentration, extraction time, and length of the hollow fiber were optimized. Under the optimized conditions, the linear range of osthole in rat plasma was 5-500ngmL -1 (r 2=0.9997). The limit of detection (LOD) was 2ngmL -1 (S/N=3) with limit of quantification (LOQ) being 5ngmL -1. The validated method has been successfully applied for pharmacokinetic studies of osthole from cerebral ischemia reperfusion rat plasma after oral administration. © 2011.


Zhou J.,Urumqi General Hospital of PLA | Zeng P.,Urumqi General Hospital of PLA | Tu H.H.,Institute for Drug and Instrument of Xinjing Miltary Command | Wang F.Q.,PLA Fourth Military Medical University
Journal of Separation Science | Year: 2011

A simple, rapid and specific method based on cloud-point extraction (CPE) was developed to determine ampelopsin in rat plasma after oral administration by reversed-phase high-performance liquid chromatography. The non-ionic surfactant Genapol X-080 was chosen as the extract solvent. Some important parameters affecting the CPE efficiency, such as the nature and concentration of surfactant, extraction temperature and time, centrifuge time and salt effect, were investigated and optimized. Separation was accomplished using a C 18 column by gradient elution with a acetonitrile-phosphate buffer solution as the mobile phase. The detection wavelength was set at 290 nm. Under optimum conditions, the linear range of ampelopsin in rat plasma was 20-2000 ng/mL (r2=0.9996). The limit of detection was 6 ng/mL (S/N=3) with the limit of quantification being 20 ng/mL (S/N=10). The proposed method has been successfully applied for pharmacokinetic studies of ampelopsin from rat plasma after oral administration. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Zhou J.,Urumqi General Hospital of PLA | Sun J.B.,No23 Hospital Of Pla | Zheng P.,Urumqi General Hospital of PLA | Liu J.,Urumqi General Hospital of PLA | And 3 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

In this work, a new sample-preparation method based on hollow-fiber liquid-phase microextraction (HFLPME) was developed for analysis of magnoflorine in rat plasma. Analysis was accomplished by reversed-phase highperformance liquid chromatography (HPLC), with ultraviolet detection by use of a photodiode-array detector. An orthogonal array design (OAD) was found to be effective for optimization of major conditions which may affect the efficiency of HF-LPME. Under the optimized conditions (pH of donor and acceptor phases 12 and 2.0, respectively; extraction time 20 min; stirring speed 800 rpm; and addition of 10 % (w/v) salt), the preconcentration factor for magnoflorine was 355. Calibration curves with reasonable linearity (r 2≥0.9994) were obtained in the range 10-1000 ng mL -1. Intra-day and inter-day precision (RSD) were <5.5 % and the limit of detection (LOD) for the analyte was 3.0 ng mL -1 (S/N=3). The validated method was successfully used for pharmacokinetic studies of magnoflorine in rat plasma after intravenous administration. © Springer-Verlag 2012.


Zhou J.,Urumqi General Hospital of PLA | Zhang Q.,Urumqi General Hospital of PLA | Sun J.B.,No 23 Hospital Of Pla | Sun X.L.,PLA Fourth Military Medical University | Zeng P.,Urumqi General Hospital of PLA
Journal of Pharmaceutical and Biomedical Analysis | Year: 2014

A new and fast sample preparation technique based on two-phase hollow fiber liquid phase microextraction (HF-LPME) with magnetofluid was developed to quantitate and determine the four phenylethanoid glycosides (PhGs) (Echinacoside, Tubuloside B, Acteoside and Isoacteoside) in plasma after oral administration of Cistanche salsa extract. Analysis was accomplished by reversed-phase high performance liquid chromatography (RP-HPLC) with ultraviolet detection. Parameters that affect the HF-LPME processes, such as the content of magnetic powder, the solvent type, salt content, stirring speed, extraction time and hollow fiber length, were investigated and optimized. Under the optimized conditions, the preconcentration factors for PhGs were higher than 625. The calibration curve for PhGs was linear in the range of 0.1-100ngmL-1 with correlation coefficients greater than 0.9996. The intra-day and inter-day precision (RSD) were below 8.74% and the limits of detection (LOD) for the four PhGs were 8-15pgmL-1 (S/N=3). The validated method was successfully applied to separate and determine the four PhGs in rat plasma after oral administration of C. salsa extract. © 2014.


Zhou J.,Urumqi General Hospital of PLA | Zeng P.,Urumqi General Hospital of PLA | Sun J.B.,No 23 Hospital Of Pla | Wang F.Q.,PLA Fourth Military Medical University | Zhang Q.,Urumqi General Hospital of PLA
Journal of Pharmaceutical and Biomedical Analysis | Year: 2013

A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction (HF-LPME) was developed and used to quantify the echinacoside in Parkinson's disease rat plasma following oral administration. Analysis was accomplished by reversed-phase high performance liquid chromatography (HPLC) with ultraviolet detection. The influence factors relevant to the HF-LPME processes were optimized. Under the optimized conditions, the preconcentration factor for echinacoside was 337. Calibration curves with reasonable linearity (r2≥0.9998) were obtained in the range of 5-750ngmL-1. Intra-day and inter-day precision (RSD) were ≤5.43% and the limit of detection (LOD) for the analyte was 2.0ngmL-1 (S/N=3). The validated method has been successfully applied for pharmacokinetic studies of echinacoside in Parkinson's disease (PD) rat plasma after oral administration. © 2013 .


Ji X.,PLA Fourth Military Medical University | Liu W.,PLA Fourth Military Medical University | Xie K.,Tianjin Medical University | Qu Y.,PLA Fourth Military Medical University | And 4 more authors.
Brain Research | Year: 2010

Traumatic brain injury (TBI) is a leading cause of mortality and disability among the young population. It has been shown that hydrogen gas (H2) exerts a therapeutic antioxidant activity by selectively reducing hydroxyl radical (OH, the most cytotoxic ROS). Recently, we have found that H2 inhalation significantly improved the survival rate and organ damage of septic mice. In the present study, we investigated the effectiveness of H2 therapy on brain edema, blood-brain barrier (BBB) breakdown, neurological dysfunction and injury volume in TBI-challenged rats. In addition, we investigated the effects of H2 treatment on the changes of oxidative products and antioxidant enzymes in brain tissue of TBI-challenged rats. Hydrogen treatment was given by exposure to 2% H2 from 5 min to 5 h after sham or TBI operation, respectively. Here, we found that TBI-challenged rats showed significant brain injuries characterized by the increase of BBB permeability, brain edema and lesion volume as well as neurological dysfunction, which was significantly attenuated by 2% H2 treatment. In addition, we found that the decrease of oxidative products and the increase of endogenous antioxidant enzymatic activities in the brain tissue may be associated with the protective effects of H2 treatment in TBI-challenged rats. The present study supports that H2 inhalation may be a more effective therapeutic strategy for patients with TBI. © 2010 Elsevier B.V. All rights reserved.


Song R.J.,Shanxi University | Zhou J.,Urumqi General Hospital of PLA
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

A simple, cost-effective, and efficient method was developed for the rapid simultaneous separation and determination of six flavonoids (rutin, hyperoside, quercetin-3-O-sophoroside, isoquercitrin, astragalin and quercetin) of Apocynum venetum leaf extract by reversed phase high performance liquid chromatography using a microemulsion system mixture as the mobile phase. Separations were performed on the Zorbax Extend-C18 column with UV detection at 360 nm. The flow rate was 0.8 mL min-1. The optimized microemulsion mobile phase consisted of 2.5% (v/v) n-butanol, 1.2% (v/v) of Genapol X-080, 0.5% (v/v) ethyl acetate and 95.8% (w/v) of aqueous 20 mM phosphoric acid, pH adjusted to 6.0 with 0.3% triethylamine. Under the optimized conditions, the calibration curve for six flavonoids was linear in the range of 5-1000μgmL-1 with the correlation coefficients greater than 0.9994. The intra-day and inter-day precision (RSD) were below 8.11% and the limits of detection (LOD) for the six flavonoids were 1.7-6.0 μg mL-1 (S/N=3). The microemulsion liquid chromatography (MELC) method was successfully applied to separate and determine the six flavonoids of A. venetum leaf extract. © 2015 Elsevier B.V.


Wang Y.F.,Emory University | Fu J.,Urumqi General Hospital of PLA
Journal of Thoracic Disease | Year: 2014

It is still challenging to prevent and treat respiratory infectious diseases. One critical step in the successful treatment of respiratory infections is rapid diagnosis by identifying the causative microorganisms in a timely fashion. However, traditional methods for identification of causative agents could not satisfy the need for rapid and accurate testing due to the limitations of technology-used. In recent years, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) has been validated and used for rapid identification of microorganism and for potential discovery of diseases associated biomarkers. We reviewed recent advances of MALDI-TOF-MS as the laboratory diagnostic tool for the rapid laboratory diagnosis of microorganisms associated with respiratory infectious diseases, with the focus on rapid identification of pathogenic bacteria and molecular markers discovery using MALDI-TOF-MS. With the advanced technologies such as MALDI-TOF, early and targeted therapies based on rapid identification of pathogens and could lead to quick and effective treatment of respiratory infections and better patient management. © Pioneer Bioscience Publishing Company.

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