Urals State Medical Academy

Yekaterinburg, Russia

Urals State Medical Academy

Yekaterinburg, Russia
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Lvova O.A.,Urals State Medical Academy | Kovtun O.P.,Urals State Medical Academy | Chegodaev D.A.,Urals State Medical Academy
Neuroscience and Behavioral Physiology | Year: 2013

We present here results from studies of the histories of thrombosis and polymorphisms of the hemocoagulation system genes in 27 children with ischemic stroke. The structures of allelic variants are compared in the Russian and non-Russian populations. All patients had more than four procoagulatory mutations. The most frequent were polymorphisms of the FGB fibrinogen gene (G-455A), the ITGA2 thrombocyte receptor gene (C807T), and the PAI-1 fibrinolysin gene (675 4G4G). The family histories of 81.5% of the children included thrombotic episodes and vascular events in relatives aged less than 50 years. Current data on the frequencies of mutations in populations of sick and healthy children are analyzed, along with their phenotypic characteristics. © 2013 Springer Science+Business Media New York.


Shklyar T.F.,Ural Federal University | Toropova O.A.,Urals State Medical Academy | Safronov A.P.,Ural Federal University | Pollack G.H.,University of Washington | Blyakhman F.A.,Ural Federal University
Biophysics | Year: 2011

A physical model of the cytoskeleton based on synthetic polyelectrolyte hydrogel of polymethacrylic acid has been proposed. From the physicochemical point of view, the structures of polyelectrolyte gel and the cytoskeleton show a high degree of similarity. It has been shown that polyelectrolyte gel can shorten and produce mechanical stress in response to changes in the composition of the surrounding solution. The mechanical properties of the model gel have been evaluated: Young modulus (2-6 kPa), stress relaxation time (0. 1-1 s), and apparent viscosity (0. 3-3 kPa s). The viscoelastic properties of the gel depend on the degree of its swelling. It has been demonstrated that the mechanical properties of gels of polymethacrylic acid are close to those of biological objects. © 2011 Pleiades Publishing, Ltd.


Shklyar T.F.,Ural Federal University | Safronov A.P.,Ural Federal University | Toropova O.A.,Urals State Medical Academy | Pollack G.H.,University of Washington | Blyakhman F.A.,Ural Federal University
Biophysics | Year: 2010

Mechanical and electrical properties of a synthetic polyelectrolyte hydrogel considered as a model of the cytoskeletal gel were studied. Hydrogels were synthesized from polymethacrylic acid by radical polymerization in aqueous solution. The electrical charge was introduced into the gel network by partial neutralization of monomer acids with magnesium hydroxide. Through the use of a motor, triangular longitudinal (axial) deformations were applied to gel samples. Simultaneously, the electrochemical (Donnan) potential of the gel was measured using conventional microelectrodes. We found that: (1) the Young modulus of the gel is 0.53 kPa; (2) at a given deformation velocity, the extent of gel deformation closely correlates with the gel potential; and (3) at the same level of gel deformation, the lower the deformation velocity, the higher the relative change of gel potential. These findings show a striking similarity to the data obtained in living cells, particularly in cardiac myocytes. A hypothesis involving the deformation-induced solvent migration from the gel to the surrounding solution is considered. It is concluded that the physicochemical features of the cytoskeletal gel may play a role in determining the mechanoelectric properties of excited cells. © 2010 Pleiades Publishing, Ltd.


Melnikov D.Yu.,Urals State Medical Academy
Voprosy Onkologii | Year: 2010

Our program has facilitated access to medical aid making earlier treatment of breast cancer possible. The morbidity rates in a city district being 1.5 times those of the metropolitan area, with 52-62% of early cancers among them, organ-saving operations rose from 3-5% to 16-17%. As a result, the 12-month mortality rate was 7.2%, while overall and relapse-free survival - 70.6 and 68.7%, respectively, which was comparable with results reported from advanced onco-centers.


Safronov A.P.,Ural Federal University | Shakhnovich M.,Urals State Medical Academy | Kalganov A.,University of Washington | Kamalov I.A.,Ural Federal University | And 3 more authors.
Polymer | Year: 2011

The periodic bending of polyelectrolyte hydrogels made of polyacrylamide, polyacrylic and polymethacrylic acids with 10% ionization under the DC electric field was observed in 0.8 mM CaCl2. When the field with intensity 13.5 V/cm was applied perpendicular to the longitudinal axis of the gel sample, the gel first bent to cathode, then more substantially to anode, then to cathode again and so on with damping amplitude. Within experimental error up to two periods of such oscillations were observed. The maximum amplitude of bending was approximately 30% of sample length. After 150-200 s, the gel sample finally reached a steady state slightly bent to anode. During these oscillations the volume of the sample gradually decreased by 10-30% depending on the gel. Kinetics of displacement of the free end of the gel sample could be fitted by a damped sine wave function, depending initial amplitude, final displacement, wave period, and decay time. All parameters except wave period depended on sample cross-section. The amplitude, wave period and decay time decrease with the increase of initial degree of gel swelling. The physical basis of the periodic bending is discussed based on the theoretical approach of Doi, Matsumoto and Hirose, although that theory can describe only initial cathodic and successive anodic displacement of gel. © 2011 Elsevier Ltd. All rights reserved.


Novoselov A.V.,Urals State Medical Academy | Rezaykin A.V.,Urals State Medical Academy | Sergeev A.G.,Urals State Medical Academy | Fadeyev F.A.,Urals State Medical Academy | And 2 more authors.
Virus Research | Year: 2012

Decay accelerating factor (DAF, CD55) is used by DAF-dependent (Daf+) variants of echovirus 11 (EV11) as a primary cellular receptor. The interaction of EV11 with DAF is completely reversible, therefore DAF-dependent variants require an unidentified coreceptor to initiate uncoating. Daf- variants of EV11, which do not interact with DAF, use an alternative primary cellular receptor. The aim of this study was to test the hypothesis whether the coreceptor, which is necessary for the uncoating of DAF-dependent variants, may act as an alternative primary receptor for the Daf- variants of EV11. By using the model of the two closely related daf+ and daf- clones of EV11 in rhabdomyosarcoma (RD) cell line, it was shown that a single amino acid substitution in the capsid protein VP2 could control the expression of the DAF-dependent phenotype. Anti-DAF monoclonal antibody has blocked the infection of RD cells by the DAF-dependent daf+ clone, but not by the daf- clone of EV11. Since the structural proteins of the two clones differed only in the receptor binding site for DAF, the unidentified non-DAF primary receptor for the daf- clone might have the same conformation as the uncoating coreceptor required for the daf+ clone. Despite the difference in primary receptors, both daf+ and daf- clones were equally inhibited by a monoclonal antibody to beta2-microglobulin. The monoclonal antibody B9.12.1 to class I human leukocyte antigen molecules showed no inhibitory effect in regards to either clone. The hypothesis of convergent intracellular traffic of Daf+ and Daf- variants of EV11 is discussed. © 2012 Elsevier B.V.


Sidorenko S.V.,St. Petersburg State Medical Academy | Savinova T.A.,National Agency of Clinical Pharmacology and Pharmacy | Ilyina E.N.,Karpov Institute of Physical Chemistry | Syrochkina M.A.,Urals State Medical Academy
Antibiotiki i Khimioterapiya | Year: 2011

Large-scale antipneumococcal vaccination is followed by changes in the serotype composition and level of antibiotic resistance in pneumococci. Hie aim of the study was to evaluate the serotype composition and population pattern of pneumococci with lower susceptibility to penicillin before large-scale antipneumococcal vaccination. Among 260 Streptococcus pneumoniae strains isolated in the Russian Federation within 2003-2007, serotypes 23F (37.2%) and 19F (13.9%) were the most frequent ones. 19.3% of the isolates belonged to serogroup 6,3.6% of the isolates each belonged to serotype 3 and serogroup 18,4.9% of the isolates belonged to serotype 14 and 2.2% of the isolates belonged to serotype 19A. 66.8% of the isolates belonged to serotypes of the 7-valent conjugated pneumococcal vaccine, 67.3 and 82.1% of the isolates belonged to the 10- and 13-valent conjugated pneumococcal vaccines respectively. Hie isolates with lower susceptibility to penicillin were characterized by significant clonality and 56.9% of them belonged to 4 global clonal complexes (CC81, CC156, CC320 and CC31S). Inclusion of the conjugated antipneumococcal vaccine to the National Vaccination Time-Table of the Russian Federation could promote lower levels of antibiotic resistance in pneumococci.


Verevshchikov V.K.,Urals State Medical Academy | Borzunov V.M.,Urals State Medical Academy | Shemyakina E.K.,Urals State Medical Academy
Antibiotiki i Khimioterapiya | Year: 2011

The therapeutic efficacy and tolerance of ergoferon, an antiviral medication combining anti-inflammatory and antihistaminic actions were estimated in the treatment of adult patients with influenza and acute respiratory viral infection. Ergoferon was shown to stimulate production of endogenous interferon-α and interferon-γ and to normalize the T-lymphocyte subpopulation composition and histamine level, that made it possible to lower the time of the disease clinical signs development and to prevent typical complications and chronic somatic pathology exacerbation.


Katsnelson B.A.,RAS Institute of High Temperature Electrochemistry | Privalova L.I.,Medical Research Center for Prophylaxis and Health Protection in Industrial Workers | Kireyeva Y.P.,Medical Research Center for Prophylaxis and Health Protection in Industrial Workers | Yeremenko O.S.,Medical Research Center for Prophylaxis and Health Protection in Industrial Workers | And 5 more authors.
Medicina del Lavoro | Year: 2012

Background: Combined toxicity of lead andfluoride has been studied insufficiently, and there is no known information about attempts to inhibit it with any bioprotectors. Methods: Lead acetate and sodium fluoride, administered separately or in combination, were injected i.p. to rats at isoeffective sublethal doses 3 times a week for 6 weeks. Some of the rats were exposed to the same combination against the background of oral administration of a bioprotector complex (BPC) comprising pectin, glutamate, and multivitamin/multimineral preparations. Following exposure, functional and biochemical indices and histopathological examinations of the femur of exposed and control rats were evaluatedfor signs of toxicity. Results: We have shown that with regard to a number of effects on the organism level the combined toxicity of lead andfluoride may be evaluated as additive or even superadditive, but lead reduces fluoride accumulation in the bone, and pathological changes in the bone tissue proved to be less marked for combined exposure compared with separate exposures. The BPC has been demonstrated to attenuate a range of the combined harmful effects of lead andfluoride, including those on the bone tissue. Conclusions: In spite of the fact that fluoride and lead may reciprocally attenuate their harmful effects on the bone tissue in case of combined exposure, they prove to be more toxic for soft tissues just in combination than when administered separately. The development of combined intoxication may be substantially inhibited by means of the tested set of innocuous biologically active agents.


PubMed | Urals State Medical Academy
Type: Journal Article | Journal: Virus research | Year: 2012

Decay accelerating factor (DAF, CD55) is used by DAF-dependent (Daf+) variants of echovirus 11 (EV11) as a primary cellular receptor. The interaction of EV11 with DAF is completely reversible, therefore DAF-dependent variants require an unidentified coreceptor to initiate uncoating. Daf- variants of EV11, which do not interact with DAF, use an alternative primary cellular receptor. The aim of this study was to test the hypothesis whether the coreceptor, which is necessary for the uncoating of DAF-dependent variants, may act as an alternative primary receptor for the Daf- variants of EV11. By using the model of the two closely related daf+ and daf- clones of EV11 in rhabdomyosarcoma (RD) cell line, it was shown that a single amino acid substitution in the capsid protein VP2 could control the expression of the DAF-dependent phenotype. Anti-DAF monoclonal antibody has blocked the infection of RD cells by the DAF-dependent daf+ clone, but not by the daf- clone of EV11. Since the structural proteins of the two clones differed only in the receptor binding site for DAF, the unidentified non-DAF primary receptor for the daf- clone might have the same conformation as the uncoating coreceptor required for the daf+ clone. Despite the difference in primary receptors, both daf+ and daf- clones were equally inhibited by a monoclonal antibody to beta2-microglobulin. The monoclonal antibody B9.12.1 to class I human leukocyte antigen molecules showed no inhibitory effect in regards to either clone. The hypothesis of convergent intracellular traffic of Daf+ and Daf- variants of EV11 is discussed.

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