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Patent
Upfront Chromatography | Date: 2017-06-21

The present invention relates to a method for providing an alpha-lactalbumin fraction and a beta-lactoglobulin fraction from a whey material obtained from milk, the method comprising the steps of: (i) providing the whey material; (ii) contacting the whey material with a chromatographic support allowing beta-lactoglobulin to be retained by the chromatographic support; (iii) obtaining a permeate fraction from the chromatographic support comprising the alpha-lactalbumin fraction; (iv) optionally washing the chromatographic support; and (v) obtaining a retentate fraction from the chromatographic support comprising the beta-lactoglobulin fraction; wherein the whey material provided in step (i) has been depleted, or substantially depleted from at least one whey protein, such as at least 2 whey proteins, e.g. at least 3 whey proteins.


Grant
Agency: European Commission | Branch: H2020 | Program: BBI-RIA | Phase: BBI.VC3.R7 | Award Amount: 3.10M | Year: 2015

There is a global need, from sustainability, food security and also health perspective, to increase dietary intake of plant protein. Side-streams from wheat and rice processing offer large under-exploited raw material potential, and we will work throughout the agro-industrial value chain to valorise that. The main aim of PROMINENT is to develop techno-economically and environmentally viable protein-based ingredients and foods from cereal processing side streams. We will concentrate on novel fractionation and extraction technologies, such as bioprocessing, supercritical carbon dioxide (SC-CO2) -extraction, thermo-mechanical technologies, wet and dry fractionation, and expanded bed adsorption as well as their combinations as novel hybrid processing technologies. A strong focus will be in using enzymatic and thermo-mechanical methods to improve techno-functional and sensory properties of protein ingredients to reach desirable taste and texture in food applications. Pasta, biscuit, cake and beverage food models are the main end product categories, where new protein ingredients will be used as dietary protein source and act as performance proteins to deliver similar techno-functional and sensory properties to animal proteins. We will also assess the safety, quality, techno-economical feasibility, sustainability and market potential of the new protein ingredients and foods, and design strategies for marketing, dissemination, and exploitation of innovations. The project will support the economic growth, resource efficiency and sustainability of the European agro-food industry, and open new market opportunities by generating new plant protein ingredients obtained from cereal processing side streams as well as new products for the consumer food market.


Lihme A.,Upfront Chromatography | Hansen M.B.,Upfront Chromatography | Andersen I.V.,Upfront Chromatography | Burnouf T.,Human Protein Process science HPPS
Analytical Biochemistry | Year: 2010

Current plasma fractionation technology combines ethanol precipitation with packed bed chromatography. We have developed a novel core fractionation process comprising five expanded bed adsorption (EBA) chromatographic steps on high-density modified agarose/tungsten carbide beads. Plasma was first chromatographed on two diethyl amino-ethyl (DEAE)-tungsten carbide agarose adsorbents (respective mean particle diameters of dv(0.5)=190 and 37μm) to isolate at 50 to 80% recovery a fraction containing 4 to 7IU/ml factor II (FII), factor IX (FIX), and factor X (FX) (specific activity >1IU/mg) and another enriched in FVIII and von Willebrand factor (vWF) (∼1IU/ml and 0.6IU/mg, respectively). The flow-through was adsorbed on 4% agarose-10% tungsten carbide beads coupled with an acidic mixed-mode ligand to isolate an 80% pure immunoglobulin G (IgG) at a 93% step recovery. A highly purified α1-antitrypsin was isolated at 95% step recovery by adsorbing the flow-through on 4% epoxy-crosslinked agarose-10% tungsten carbide adsorbent material coupled with a cationic ligand. Isolation of 98% pure albumin was achieved at a 99% step recovery by pH 4.5 adsorption of the flow-through on 6% agarose-10% tungsten carbide beads coupled with an acidic mixed-mode ligand. EBA may represent a feasible alternative core plasma fractionation tool. © 2009 Elsevier Inc.


Patent
Upfront Chromatography | Date: 2014-07-16

The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspension from step (iii) to an expanded bed adsorption process obtaining the purified protein product.


Patent
Upfront Chromatography | Date: 2011-01-26

The present invention provides a process for the isolation of one or more proteins from a protein solution. The process comprises the steps of: (a) providing a protein solution comprising one or more specific proteins and having a preset pH and a preset ionic strength or conductivity, (b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and (c) obtaining one or more proteins from the column, wherein the protein solution has been supplemented with an alcohol.


Patent
Upfront Chromatography | Date: 2011-01-12

A primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an expanded bed adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high-density non-porous core, surrounded by a porous material; the absorbent having a particle density of at least 1.5g/ml, wherein said high-density non-porous core has a density of at least 4 g/ml, and the degree of expansion (H/H0) of the adsorbent is in the range of 1.2-5, and wherein the flow rate of applying said protein-containing mixture is at least 8 cm/min; c) optionally washing the column; d) eluting at least one protein from the adsorbent.


Patent
Upfront Chromatography | Date: 2013-10-23

The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.


Patent
Upfront Chromatography | Date: 2015-01-29

The invention provides a process for the separation of pea protein. The process begins with an aqueous extract or solution of pea protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of pea protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest are isolated by eluting them from said adsorbent resin. The invention also provides various protein compositions obtainable via the method of the invention.


Patent
Upfront Chromatography | Date: 2014-11-12

The present invention relates to an -enriched whey protein isolate being low in -lactoglobulin and high in -lactalbumin and immunoglobulin G.


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