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Kumar V.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Chakravarty A.K.,National Dairy Research Institute
Buffalo Bulletin | Year: 2016

The aim of the study was to investigate efficiency of test-day model (TDM) compared to lactation model (LM) for genetic evaluation of Murrah buffalo bulls. Use of TDM instead of LM is of more interest in genetic evaluation because of variability of lactation days in dairy animals. Data pertaining to first lactation monthly test-day milk yield (FLMTDMY) and first lactation 305-days or less milk yield (FL305DMY) of 1105 Murrah buffaloes during 1993 to 2010 were collected and adjusted against significant environmental influences. It was found that test-day6 milk yield (FLMTD6MY) had the highest genetic and phenotypic correlation with FL305DMY. An attempt is being made in the present investigation to compare the estimated breeding values (EBVs) of Murrah bulls through contemporary comparison method for FL305DMY and FLMTD6MY. The rank correlations between two traits were highly statistically significant indicating that FLMTD6MY equally effective to discriminate amongst sires. © 2016, Kasetsart University. All rights reserved. Source


Yadav R.S.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan
Indian Journal of Animal Sciences | Year: 2016

Comparative disposition kinetics of ofloxacin following intramuscular (i.m.) and subcutaneous (s.c.) administration was studied in goats @ 10 mg/kg. Ofloxacin was determined by HPLC method and kinetic parameters using a software. Appreciable plasma concentrations were observed within 2.5 min of drug administration and peaked at 1 h. t1/2ka and t1/2? values were 13.59 and 78.41 min after i.m. administration while 17.89 and 103.59 min after s.c. administration and the corresponding bioavailability values were 75.43±5.00 and 102.07±7.00 %. Ofloxacin may be adiministered to goat @10 mg/kg at 12 h interval by s.c. route. Source


Singh K.P.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Saxena A.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Kharche S.D.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Singh P.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan
Indian Journal of Animal Sciences | Year: 2012

The present study was undertaken to estimate effect of granulosa cell monolayer and oviductal epithelial cells coculture on cleavage rate and embryo development of in-vitro matured and fertilized goat oocytes. The oocytes were matured in maturation media (TCM-199). After 27 h, matured oocytes were picked up and denuded and then transferred in fert-TALP medium. The capacitated sperm having concentration of 1×106 live sperm/ml were used for in-vitro fertilization. Oocytes were co-incubated with capacitated spermatozoa for > 8 h at 38.5±1°C in an atmosphere of 5% CO2 in humidified air. The fertilized oocytes culture in EDM over their respective cell culture i.e. granulosa cells monolayer and oviductal epithelial cells were obtained for further cleavage rate and embryo development (2, 4, 8, and > 8 celled) under steriozoom microscope at every 24 h with 50% replacement of pre-equilibrated EDM media up to 7 days. The cleavage rates with granulosa cells monolayer and oviductal epithelial cells co-culture, 48 h post insemination, were 43.52 and 45.27% respectively for goat oocytes. The present study indicated that both granulosa cell monolayer and oviductal epithelial cells co-culture behaved similarly for cleavage rate of in-vitro matured and fertilized goat oocytes, and oviductal epithelial cells co-culture significantly increased in embryonic development stage from 4 cell to > 8 cell stage (4 cell, 8 cell and > 8 cell) as compared to granulosa cell monolayer whereas granulosa cell monolayer coculture significantly increased the embryonic development of 2 cell stage as compared to oviductal epithelial cells. Source


Sharma D.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Singh S.K.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Srivastava M.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | Sachan P.,UP Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan | And 2 more authors.
Indian Journal of Animal Sciences | Year: 2014

Indigenous cattle that were in early lactation and positive for subclinical mastitis were allocated into 2 groups; one group was administered with nutrional supplements (50 g mixture of vitamins A, D, E and thiamine, riboflavin, pantothenic acid, biotin, niacin, trisodium citrate dihydrate, methionine, manganese, copper, zinc, cobalt, selenium and live yeasts orally daily for 7 days), while other was kept as negative control. Milk composition of mastitic milk and metabolic status of affected cows were evaluated at day 0 and day 7 post-therapy. On day 0, remarkable alteration in milk composition as well as in metabolic status of affected animals was recorded in comparison to the healthy control. However, the altered nutrional panels as well as milk compositions were ameliorated toward normalcy at day 7 post-therapy in mastitic cows administered with nutrional supplements. At day 7 post-therapy, remarkable improvements in somatic cell count was also recorded in these cows when compared with day 0 values within the group, but the values were still significantly higher than the healthy control. Thus, subclinical mastitis in indigenous cattle could bestow remarkable alterations in milk compositions and metabolic status. The altered metabolic panels and milk compositions can be ameliorated toward normalcy by administering nutritional supplements. Source

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