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Swain D.K.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Swarnkar P.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Yadav S.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya
Indian Journal of Animal Sciences | Year: 2012

The study was designed to find a suitable HOS solution concentration for the sperms retrieved from 3 segments of epididymis. The major aim of the study was to evaluate the pattern of osmotic resistance of the epididymal sperms to different concentrations of hypo-osmotic solutions. We reported the highest sperm coiling at 75 mOsm/L for the caput and corpus sperms whereas cauda sperms exhibited highest coiling at 125mOsm/L. The osmotic resistance of the cauda sperms was greater than that of the caput and corpus. Further studies are required to study the exact mechanism behind the sperm membrane integrity and its relationship with the epididymal transit and how the osmotic resitance of the sperms gradually increased during the epididymal transit. Source


Swain D.K.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Swarnkar P.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Kumar J.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Yadav S.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya
Indian Journal of Animal Sciences | Year: 2012

The present study was designed to evaluate the effect of both temperature and storage time interval on the cauda epididymal sperm quality in bucks. The testes collected in normal saline at 4°C were exhibited improved sperm attributes at all periods of storage temperature as compared to the testes collected in normal saline at room temperature as well as preserved at the same temperature. In both cases, with the passage of time of storage the sperm features started deteriorating. That is why the study recommended that to maintain the sperm attributes for a longer period of storage, suitable antioxidants and amino acids may be supplemented. The sperms should be isolated as soon as possible after the collection of the testes to maintain the integrity and quality of the sperm from the cauda. It was evident that, as soon as possible, the sperms from the cauda should be recovered as well as storage temperature should be lesser than 4°C. Suitable strategies should be developed to cryopreserve the cauda epididymal sperms. Source


Chauhan A.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Tiwari M.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Singh S.P.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | Sharma D.,UP Pt Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya | And 4 more authors.
Indian Journal of Animal Sciences | Year: 2015

In the present study, identification of PIT-1 gene polymorphism and its association with milk production traits was undertaken in 77 Sahiwal cattle maintained at NDRI, Karnal using PCR-RFLP. Amplification of DNA sample revealed 600 bp product and restriction digestion with HinfI showed three types of genotypes, namely, AA (600 bp), AB (600, 343 and 257 bp) and BB (343 and 257 bp) genotypes. The frequency of BB genotypes was highest (64.98%) in all screened samples, followed by AB genotype (31.16%) and AA genotype (3.89%). The allelic frequency of PIT-1 A and B alleles were 19.48% and 80.51%, respectively. Association studies of PIT-1 gene with milk production traits showed that age at first calving, gestation period and lactation period had non-significant variation among all the 3 genotypes up to fourth lactation. However, a significant difference was found among the 3 genotypes for total milk yield and milk yield at 300 days with AA genotype showing higher value than AB and BB genotypes in the first lactation. From present investigation, the HinfI /PCR-RFLP revealed polymorphic pattern of PIT-1 gene in Sahiwal cattle and association studies showed significant effect of A allele on milk production. © 2015, Indian Council of Agricultural Research. All rights reserved. Source

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